Novel Autoantibodies that recognize native transfer RNAs in patients with systemic rheumatic diseases

M. Matsumura, Y. Ohosone, M. Ishida, Y. Takahashi, Michito Hirakata, T. Mimori

Research output: Contribution to journalArticle

Abstract

Autoantibodies directed against aminoacyl-tRNA synthetases are well described in adult polymyositis/dermatomyositis. However, the characteristics of antibodies against other tRNA and tRNA-associated proteins have not been well defined. In this study, we identified novel autoantibodies to total tRNAs in patients with systemic rheumatic diseases and characterized the structure that was recognized by anti-tRNA antibodies. We identified fifteen patients sera that immunoprecipitated the deproteinized cognate tRNA. Two of them were identified as having previously well-defined anti-PL-12 antibodies. Three of the remaining 13 patient sera immunoprecipitated naked tRNA of E. coli. To investigate the antibody binding site on tRNAs, we have used in vitro transcripts from cDNAs encoding wild type tRNAs of E. coli and their synthesized mutants. These sera revealed different reactivity to mutated tRNAs. Eleven of these 13 patients met disease specific criteria for: SLE (3 patients), Sjogren's syndrome (6), or SLE/Sjogren's syndrome overlap (2). In addition, fever, Raynaud's phenomenon, and non-erosive polyarthritis, were frequently found in these patients. In summary, we have identified novel antibodies to tRNAs which appear to be quite common, and distinct from previously described anti-aminoacyl-tRNA synthetase antibodies.

Original languageEnglish
Pages (from-to)556-563
Number of pages8
JournalRyumachi
Volume37
Issue number4
Publication statusPublished - 1997

Fingerprint

Transfer RNA
Rheumatic Diseases
Autoantibodies
Amino Acyl-tRNA Synthetases
Antibodies
Sjogren's Syndrome
Serum
Antibody Binding Sites
Escherichia coli
Raynaud Disease
Dermatomyositis
Arthritis
Anti-Idiotypic Antibodies
Fever
Complementary DNA

Keywords

  • Autoantibody
  • Connective tissue disease
  • Transfer RNA

ASJC Scopus subject areas

  • Rheumatology

Cite this

Matsumura, M., Ohosone, Y., Ishida, M., Takahashi, Y., Hirakata, M., & Mimori, T. (1997). Novel Autoantibodies that recognize native transfer RNAs in patients with systemic rheumatic diseases. Ryumachi, 37(4), 556-563.

Novel Autoantibodies that recognize native transfer RNAs in patients with systemic rheumatic diseases. / Matsumura, M.; Ohosone, Y.; Ishida, M.; Takahashi, Y.; Hirakata, Michito; Mimori, T.

In: Ryumachi, Vol. 37, No. 4, 1997, p. 556-563.

Research output: Contribution to journalArticle

Matsumura, M, Ohosone, Y, Ishida, M, Takahashi, Y, Hirakata, M & Mimori, T 1997, 'Novel Autoantibodies that recognize native transfer RNAs in patients with systemic rheumatic diseases', Ryumachi, vol. 37, no. 4, pp. 556-563.
Matsumura, M. ; Ohosone, Y. ; Ishida, M. ; Takahashi, Y. ; Hirakata, Michito ; Mimori, T. / Novel Autoantibodies that recognize native transfer RNAs in patients with systemic rheumatic diseases. In: Ryumachi. 1997 ; Vol. 37, No. 4. pp. 556-563.
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