Novel axonal projection from the caudal end of the ventrolateral medulla to the intermediolateral cell column

Kamon Iigaya, Hiroo Kumagai, Hiroshi Onimaru, Akira Kawai, Naoki Oshima, Toshiko Onami, Chie Takimoto, Tadashi Kamayachi, Koichi Hayashi, Takao Saruta, Hiroshi Itoh

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We used an optical imaging technique to investigate whether axons of neurons in the caudal end of the ventrolateral medulla (CeVLM), as well as axons of neurons in the rostral ventrolateral medulla (RVLM), project to neurons in the intermediolateral cell column (IML) of the spinal cord. Brain stem-spinal cord preparations from neonatal normotensive Wistar-Kyoto and spontaneously hypertensive rats were stained with a voltage-sensitive dye, and responses to electrical stimulation of the IML at the Th2 level were detected as changes in fluorescence intensity with an optical imaging apparatus (MiCAM-01). The results were as follows: 1) depolarizing responses to IML stimulation during low-Ca high-Mg superfusion were detected on the ventral surface of the medulla at the level of the CeVLM, as well as at the level of the RVLM, 2) depolarizing responses were also detected on cross sections at the level of the CeVLM, and they had a latency of 24.0 ± 5.5 (SD) ms, 3) antidromic action potentials in response to IML stimulation were demonstrated in the CeVLM neurons where optical images were detected, and 4) glutamate application to the CeVLM increased the frequency of excitatory postsynaptic potentials (EPSPs) and induced depolarization of the IML neurons. The optical imaging findings suggested a novel axonal and functional projection from neurons in the CeVLM to the IML. The increase in EPSPs of the IML neurons in response to glutamate application suggests that the CeVLM participates in the regulation of sympathetic nerve activity and blood pressure and may correspond to the caudal pressor area.

Original languageEnglish
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume292
Issue number2
DOIs
Publication statusPublished - 2007 Feb

Fingerprint

Neurons
Optical Imaging
Excitatory Postsynaptic Potentials
Axons
Glutamic Acid
Spinal Cord
Inbred SHR Rats
Spinal Cord Lateral Horn
Electric Stimulation
Action Potentials
Brain Stem
Coloring Agents
Fluorescence
Blood Pressure

Keywords

  • Caudal pressor area
  • Monosynaptic bulbospinal projection
  • Optical imaging
  • Patch-clamp technique
  • Sympathetic nervous system

ASJC Scopus subject areas

  • Physiology

Cite this

Novel axonal projection from the caudal end of the ventrolateral medulla to the intermediolateral cell column. / Iigaya, Kamon; Kumagai, Hiroo; Onimaru, Hiroshi; Kawai, Akira; Oshima, Naoki; Onami, Toshiko; Takimoto, Chie; Kamayachi, Tadashi; Hayashi, Koichi; Saruta, Takao; Itoh, Hiroshi.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 292, No. 2, 02.2007.

Research output: Contribution to journalArticle

Iigaya, Kamon ; Kumagai, Hiroo ; Onimaru, Hiroshi ; Kawai, Akira ; Oshima, Naoki ; Onami, Toshiko ; Takimoto, Chie ; Kamayachi, Tadashi ; Hayashi, Koichi ; Saruta, Takao ; Itoh, Hiroshi. / Novel axonal projection from the caudal end of the ventrolateral medulla to the intermediolateral cell column. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 2007 ; Vol. 292, No. 2.
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AU - Iigaya, Kamon

AU - Kumagai, Hiroo

AU - Onimaru, Hiroshi

AU - Kawai, Akira

AU - Oshima, Naoki

AU - Onami, Toshiko

AU - Takimoto, Chie

AU - Kamayachi, Tadashi

AU - Hayashi, Koichi

AU - Saruta, Takao

AU - Itoh, Hiroshi

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N2 - We used an optical imaging technique to investigate whether axons of neurons in the caudal end of the ventrolateral medulla (CeVLM), as well as axons of neurons in the rostral ventrolateral medulla (RVLM), project to neurons in the intermediolateral cell column (IML) of the spinal cord. Brain stem-spinal cord preparations from neonatal normotensive Wistar-Kyoto and spontaneously hypertensive rats were stained with a voltage-sensitive dye, and responses to electrical stimulation of the IML at the Th2 level were detected as changes in fluorescence intensity with an optical imaging apparatus (MiCAM-01). The results were as follows: 1) depolarizing responses to IML stimulation during low-Ca high-Mg superfusion were detected on the ventral surface of the medulla at the level of the CeVLM, as well as at the level of the RVLM, 2) depolarizing responses were also detected on cross sections at the level of the CeVLM, and they had a latency of 24.0 ± 5.5 (SD) ms, 3) antidromic action potentials in response to IML stimulation were demonstrated in the CeVLM neurons where optical images were detected, and 4) glutamate application to the CeVLM increased the frequency of excitatory postsynaptic potentials (EPSPs) and induced depolarization of the IML neurons. The optical imaging findings suggested a novel axonal and functional projection from neurons in the CeVLM to the IML. The increase in EPSPs of the IML neurons in response to glutamate application suggests that the CeVLM participates in the regulation of sympathetic nerve activity and blood pressure and may correspond to the caudal pressor area.

AB - We used an optical imaging technique to investigate whether axons of neurons in the caudal end of the ventrolateral medulla (CeVLM), as well as axons of neurons in the rostral ventrolateral medulla (RVLM), project to neurons in the intermediolateral cell column (IML) of the spinal cord. Brain stem-spinal cord preparations from neonatal normotensive Wistar-Kyoto and spontaneously hypertensive rats were stained with a voltage-sensitive dye, and responses to electrical stimulation of the IML at the Th2 level were detected as changes in fluorescence intensity with an optical imaging apparatus (MiCAM-01). The results were as follows: 1) depolarizing responses to IML stimulation during low-Ca high-Mg superfusion were detected on the ventral surface of the medulla at the level of the CeVLM, as well as at the level of the RVLM, 2) depolarizing responses were also detected on cross sections at the level of the CeVLM, and they had a latency of 24.0 ± 5.5 (SD) ms, 3) antidromic action potentials in response to IML stimulation were demonstrated in the CeVLM neurons where optical images were detected, and 4) glutamate application to the CeVLM increased the frequency of excitatory postsynaptic potentials (EPSPs) and induced depolarization of the IML neurons. The optical imaging findings suggested a novel axonal and functional projection from neurons in the CeVLM to the IML. The increase in EPSPs of the IML neurons in response to glutamate application suggests that the CeVLM participates in the regulation of sympathetic nerve activity and blood pressure and may correspond to the caudal pressor area.

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KW - Monosynaptic bulbospinal projection

KW - Optical imaging

KW - Patch-clamp technique

KW - Sympathetic nervous system

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