Novel conserved structural domains of acrosome reaction-inducing substance are widespread in invertebrates

Masahiro Naruse, Riho Ishikawa, Hiroshi Sakaya, Hideaki Moriyama, Motonori Hoshi, Midori Matsumoto

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In the starfish Asterias amurensis, acrosome reaction inducing substance (ARIS) is the main factor responsible for allowing sperm to recognize the egg jelly and begin the acrosome reaction (AR). ARIS is a large proteoglycan-like molecule, and its pentasaccharide repeat, Fragment 1 (Fr. 1), is responsible for inducing AR. Here, we investigated the primary structure of ARIS for the first time in order to improve our understanding of its functionality. Electrophoretic analysis revealed that ARIS is a complex of three proteins, all of which are modified by the Fr. 1 sugar chain. Sequencing indicated that there are two novel, conserved domains in all three ARIS proteins: ARIS N-terminus (AR-N) and ARIS C-terminus (AR-C) domains. We also found that other echinoderms possess ARIS proteins that are capable of inducing the AR for homologous sperm, indicating that ARIS proteins may be a ubiquitous component for echinoderm fertilization. Moreover, we identified ARIS-like genes from Ctenophora to Protochordata.

Original languageEnglish
Pages (from-to)57-66
Number of pages10
JournalMolecular Reproduction and Development
Volume78
Issue number1
DOIs
Publication statusPublished - 2011 Jan

Fingerprint

Acrosome Reaction
Invertebrates
Spermatozoa
Ctenophora
Proteins
Asterias
Starfish
Proteoglycans
Fertilization
Ovum

Keywords

  • Acrosome reaction
  • Egg coat structure
  • Invertebrate
  • Sugar chain recognition

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Novel conserved structural domains of acrosome reaction-inducing substance are widespread in invertebrates. / Naruse, Masahiro; Ishikawa, Riho; Sakaya, Hiroshi; Moriyama, Hideaki; Hoshi, Motonori; Matsumoto, Midori.

In: Molecular Reproduction and Development, Vol. 78, No. 1, 01.2011, p. 57-66.

Research output: Contribution to journalArticle

Naruse, Masahiro ; Ishikawa, Riho ; Sakaya, Hiroshi ; Moriyama, Hideaki ; Hoshi, Motonori ; Matsumoto, Midori. / Novel conserved structural domains of acrosome reaction-inducing substance are widespread in invertebrates. In: Molecular Reproduction and Development. 2011 ; Vol. 78, No. 1. pp. 57-66.
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