Novel fluorescent probe for highly sensitive bioassay using sequential enzyme-linked immunosorbent assay-capillary isoelectric focusing (ELISA-cIEF)

Terence G. Henares, Yuta Uenoyama, Yuto Nogawa, Ken Ikegami, Daniel Citterio, Koji Suzuki, Shun Ichi Funano, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

This paper presents a novel rhodamine diphosphate molecule that allows highly sensitive detection of proteins by employing sequential enzyme-linked immunosorbent assay and capillary isoelectric focusing (ELISA-cIEF). Seven-fold improvement in the immunoassay sensitivity and a 1-2 order of magnitude lower detection limit has been demonstrated by taking advantage of the combination of the enzyme-based signal amplification of ELISA and the concentration of enzyme reaction products by cIEF. This journal is

Original languageEnglish
Pages (from-to)3139-3141
Number of pages3
JournalAnalyst
Volume138
Issue number11
DOIs
Publication statusPublished - 2013 Jun 7

Fingerprint

Immunosorbents
Bioassay
Isoelectric Focusing
Fluorescent Dyes
Biological Assay
bioassay
Assays
Enzymes
Enzyme-Linked Immunosorbent Assay
probe
assay
enzyme
Rhodamines
Diphosphates
Immunoassay
Limit of Detection
immunoassay
Reaction products
Amplification
amplification

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Electrochemistry
  • Biochemistry
  • Environmental Chemistry

Cite this

Novel fluorescent probe for highly sensitive bioassay using sequential enzyme-linked immunosorbent assay-capillary isoelectric focusing (ELISA-cIEF). / Henares, Terence G.; Uenoyama, Yuta; Nogawa, Yuto; Ikegami, Ken; Citterio, Daniel; Suzuki, Koji; Funano, Shun Ichi; Sueyoshi, Kenji; Endo, Tatsuro; Hisamoto, Hideaki.

In: Analyst, Vol. 138, No. 11, 07.06.2013, p. 3139-3141.

Research output: Contribution to journalArticle

Henares, TG, Uenoyama, Y, Nogawa, Y, Ikegami, K, Citterio, D, Suzuki, K, Funano, SI, Sueyoshi, K, Endo, T & Hisamoto, H 2013, 'Novel fluorescent probe for highly sensitive bioassay using sequential enzyme-linked immunosorbent assay-capillary isoelectric focusing (ELISA-cIEF)', Analyst, vol. 138, no. 11, pp. 3139-3141. https://doi.org/10.1039/c3an00098b
Henares, Terence G. ; Uenoyama, Yuta ; Nogawa, Yuto ; Ikegami, Ken ; Citterio, Daniel ; Suzuki, Koji ; Funano, Shun Ichi ; Sueyoshi, Kenji ; Endo, Tatsuro ; Hisamoto, Hideaki. / Novel fluorescent probe for highly sensitive bioassay using sequential enzyme-linked immunosorbent assay-capillary isoelectric focusing (ELISA-cIEF). In: Analyst. 2013 ; Vol. 138, No. 11. pp. 3139-3141.
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