Novel fluorescent probe for highly sensitive bioassay using sequential enzyme-linked immunosorbent assay-capillary isoelectric focusing (ELISA-cIEF)

Terence G. Henares, Yuta Uenoyama, Yuto Nogawa, Ken Ikegami, Daniel Citterio, Koji Suzuki, Shun Ichi Funano, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

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10 Citations (Scopus)

Abstract

This paper presents a novel rhodamine diphosphate molecule that allows highly sensitive detection of proteins by employing sequential enzyme-linked immunosorbent assay and capillary isoelectric focusing (ELISA-cIEF). Seven-fold improvement in the immunoassay sensitivity and a 1-2 order of magnitude lower detection limit has been demonstrated by taking advantage of the combination of the enzyme-based signal amplification of ELISA and the concentration of enzyme reaction products by cIEF.

Original languageEnglish
Pages (from-to)3139-3141
Number of pages3
JournalAnalyst
Volume138
Issue number11
DOIs
Publication statusPublished - 2013 Jun 7

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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    Henares, T. G., Uenoyama, Y., Nogawa, Y., Ikegami, K., Citterio, D., Suzuki, K., Funano, S. I., Sueyoshi, K., Endo, T., & Hisamoto, H. (2013). Novel fluorescent probe for highly sensitive bioassay using sequential enzyme-linked immunosorbent assay-capillary isoelectric focusing (ELISA-cIEF). Analyst, 138(11), 3139-3141. https://doi.org/10.1039/c3an00098b