Novel human BTB/POZ domain-containing zinc finger protein ZNF295 is directly associated with ZFP161

Jun Wang, Jun Kudo, Atsushi Takayanagi, Nobuyoshi Shimizu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Human ZNF295 protein harbors a BTB/POZ domain and multiple krüppel (C 2H 2) type zinc finger domains, and thus belongs to a family of POK (POZ and krüppel) transcription factor. We have identified many transcript variants generated by the alternative splicing in 5′ non-coding exons, an intra-exonic splicing in a coding region, and the use of three polyadenylation signals in the 3′ UTR. The intra-exonic splicing removes 603-bp coding sequence, and thus ZNF295 gene produces two protein isoforms: ZNF295L with 1066 amino acid residues and ZNF295S with 865 amino acid residues, containing 9 and 5 zinc finger domains, respectively. ZNF295 is ubiquitously expressed in human fetal and adult tissues. Analysis of transcription activity of ZNF295 using various promoter-reporters demonstrated that ZNF295 acts as a transcription repressor, and contains two separate regions for repression activity: the BTB/POZ domain and the central region between BTB/POZ and ZF domains. Both ZNF295L and ZNF295S could interact not only with themselves and each other, but also with another POK protein ZFP161 known to function as a transcription repressor and an activator. We postulated that ZNF295 may be involved in the bi-directional control of gene expression in concert with ZFP161.

Original languageEnglish
Pages (from-to)615-627
Number of pages13
JournalBiochemical and Biophysical Research Communications
Volume327
Issue number2
DOIs
Publication statusPublished - 2005 Feb 11

Fingerprint

Zinc Fingers
Transcription
Zinc
Amino Acids
Polyadenylation
Proteins
Alternative Splicing
3' Untranslated Regions
Ports and harbors
Gene expression
Exons
Protein Isoforms
Fetus
Transcription Factors
Genes
Tissue
Gene Expression
BTB-POZ Domain

Keywords

  • BTB/POZ
  • Chromosome 21q22.3
  • Transcription repressor
  • Zinc finger

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Novel human BTB/POZ domain-containing zinc finger protein ZNF295 is directly associated with ZFP161. / Wang, Jun; Kudo, Jun; Takayanagi, Atsushi; Shimizu, Nobuyoshi.

In: Biochemical and Biophysical Research Communications, Vol. 327, No. 2, 11.02.2005, p. 615-627.

Research output: Contribution to journalArticle

Wang, Jun ; Kudo, Jun ; Takayanagi, Atsushi ; Shimizu, Nobuyoshi. / Novel human BTB/POZ domain-containing zinc finger protein ZNF295 is directly associated with ZFP161. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 327, No. 2. pp. 615-627.
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