Novel human BTB/POZ domain-containing zinc finger protein ZNF295 is directly associated with ZFP161

Jun Wang, Jun Kudoh, Atsushi Takayanagi, Nobuyoshi Shimizu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Human ZNF295 protein harbors a BTB/POZ domain and multiple krüppel (C 2H 2) type zinc finger domains, and thus belongs to a family of POK (POZ and krüppel) transcription factor. We have identified many transcript variants generated by the alternative splicing in 5′ non-coding exons, an intra-exonic splicing in a coding region, and the use of three polyadenylation signals in the 3′ UTR. The intra-exonic splicing removes 603-bp coding sequence, and thus ZNF295 gene produces two protein isoforms: ZNF295L with 1066 amino acid residues and ZNF295S with 865 amino acid residues, containing 9 and 5 zinc finger domains, respectively. ZNF295 is ubiquitously expressed in human fetal and adult tissues. Analysis of transcription activity of ZNF295 using various promoter-reporters demonstrated that ZNF295 acts as a transcription repressor, and contains two separate regions for repression activity: the BTB/POZ domain and the central region between BTB/POZ and ZF domains. Both ZNF295L and ZNF295S could interact not only with themselves and each other, but also with another POK protein ZFP161 known to function as a transcription repressor and an activator. We postulated that ZNF295 may be involved in the bi-directional control of gene expression in concert with ZFP161.

Original languageEnglish
Pages (from-to)615-627
Number of pages13
JournalBiochemical and Biophysical Research Communications
Volume327
Issue number2
DOIs
Publication statusPublished - 2005 Feb 11

Keywords

  • BTB/POZ
  • Chromosome 21q22.3
  • Transcription repressor
  • Zinc finger

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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