Novel mitochondria-targeted heat-soluble proteins identified in the anhydrobiotic tardigrade improve osmotic tolerance of human cells

Sae Tanaka, Junko Tanaka, Yoshihiro Miwa, Daiki D. Horikawa, Toshiaki Katayama, Kazuharu Arakawa, Atsushi Toyoda, Takeo Kubo, Takekazu Kunieda

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Tardigrades are able to tolerate almost complete dehydration through transition to a metabolically inactive state, called "anhydrobiosis". Late Embryogenesis Abundant (LEA) proteins are heat-soluble proteins involved in the desiccation tolerance of many anhydrobiotic organisms. Tardigrades, Ramazzottius varieornatus, however, express predominantly tardigrade-unique heat-soluble proteins: CAHS (Cytoplasmic Abundant Heat Soluble) and SAHS (Secretory Abundant Heat Soluble) proteins, which are secreted or localized in most intracellular compartments, except the mitochondria. Although mitochondrial integrity is crucial to ensure cellular survival, protective molecules for mitochondria have remained elusive. Here, we identified two novel mitochondrial heat-soluble proteins, RvLEAM and MAHS (Mitochondrial Abundant Heat Soluble), as potent mitochondrial protectants from Ramazzottius varieornatus. RvLEAM is a group3 LEA protein and immunohistochemistry confirmed its mitochondrial localization in tardigrade cells. MAHS-green fluorescent protein fusion protein localized in human mitochondria and was heat-soluble in vitro, though no sequence similarity with other known proteins was found, and one region was conserved among tardigrades. Furthermore, we demonstrated that RvLEAM protein as well as MAHS protein improved the hyperosmotic tolerance of human cells. The findings of the present study revealed that tardigrade mitochondria contain at least two types of heat-soluble proteins that might have protective roles in water-deficient environments.

Original languageEnglish
Article numbere0118272
JournalPLoS One
Volume10
Issue number2
DOIs
Publication statusPublished - 2015 Feb 12

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osmotolerance
Tardigrada
Mitochondria
mitochondria
Hot Temperature
Cells
heat
Proteins
proteins
cells
Mitochondrial Proteins
Embryonic Development
embryogenesis
Desiccation
Green Fluorescent Proteins
Dehydration
green fluorescent protein
immunohistochemistry
Fusion reactions
Immunohistochemistry

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Novel mitochondria-targeted heat-soluble proteins identified in the anhydrobiotic tardigrade improve osmotic tolerance of human cells. / Tanaka, Sae; Tanaka, Junko; Miwa, Yoshihiro; Horikawa, Daiki D.; Katayama, Toshiaki; Arakawa, Kazuharu; Toyoda, Atsushi; Kubo, Takeo; Kunieda, Takekazu.

In: PLoS One, Vol. 10, No. 2, e0118272, 12.02.2015.

Research output: Contribution to journalArticle

Tanaka, Sae ; Tanaka, Junko ; Miwa, Yoshihiro ; Horikawa, Daiki D. ; Katayama, Toshiaki ; Arakawa, Kazuharu ; Toyoda, Atsushi ; Kubo, Takeo ; Kunieda, Takekazu. / Novel mitochondria-targeted heat-soluble proteins identified in the anhydrobiotic tardigrade improve osmotic tolerance of human cells. In: PLoS One. 2015 ; Vol. 10, No. 2.
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