Novel non-heme iron center of nitrile hydratase with a claw setting of oxygen atoms.

S. Nagashima, Masayoshi Nakasako, N. Dohmae, M. Tsujimura, K. Takio, M. Odaka, M. Yohda, N. Kamiya, I. Endo

Research output: Contribution to journalArticle

310 Citations (Scopus)

Abstract

The iron-containing nitrile hydratase (NHase) is a photoreactive enzyme that is inactivated in the dark because of persistent association with NO and activated by photo-dissociation of NO. The crystal structure at 1.7 A resolution and mass spectrometry revealed the structure of the non-heme iron catalytic center in the nitrosylated state. Two Cys residues coordinated to the iron were post-translationally modified to Cys-sulfenic and -sulfinic acids. Together with another oxygen atom of the Ser ligand, these modifications induced a claw setting of oxygen atoms capturing an NO molecule. This unprecedented structure is likely to enable the photo-regulation of NHase and will provide an excellent model for designing photo-controllable chelate complexes and, ultimately, proteins.

Original languageEnglish
Pages (from-to)347-351
Number of pages5
JournalNature Structural Biology
Volume5
Issue number5
Publication statusPublished - 1998 May
Externally publishedYes

Fingerprint

Hoof and Claw
Iron
Oxygen
Atoms
Sulfinic Acids
Photodissociation
Mass spectrometry
Mass Spectrometry
Crystal structure
Association reactions
Ligands
Molecules
Enzymes
nitrile hydratase
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Structural Biology
  • Genetics

Cite this

Nagashima, S., Nakasako, M., Dohmae, N., Tsujimura, M., Takio, K., Odaka, M., ... Endo, I. (1998). Novel non-heme iron center of nitrile hydratase with a claw setting of oxygen atoms. Nature Structural Biology, 5(5), 347-351.

Novel non-heme iron center of nitrile hydratase with a claw setting of oxygen atoms. / Nagashima, S.; Nakasako, Masayoshi; Dohmae, N.; Tsujimura, M.; Takio, K.; Odaka, M.; Yohda, M.; Kamiya, N.; Endo, I.

In: Nature Structural Biology, Vol. 5, No. 5, 05.1998, p. 347-351.

Research output: Contribution to journalArticle

Nagashima, S, Nakasako, M, Dohmae, N, Tsujimura, M, Takio, K, Odaka, M, Yohda, M, Kamiya, N & Endo, I 1998, 'Novel non-heme iron center of nitrile hydratase with a claw setting of oxygen atoms.', Nature Structural Biology, vol. 5, no. 5, pp. 347-351.
Nagashima S, Nakasako M, Dohmae N, Tsujimura M, Takio K, Odaka M et al. Novel non-heme iron center of nitrile hydratase with a claw setting of oxygen atoms. Nature Structural Biology. 1998 May;5(5):347-351.
Nagashima, S. ; Nakasako, Masayoshi ; Dohmae, N. ; Tsujimura, M. ; Takio, K. ; Odaka, M. ; Yohda, M. ; Kamiya, N. ; Endo, I. / Novel non-heme iron center of nitrile hydratase with a claw setting of oxygen atoms. In: Nature Structural Biology. 1998 ; Vol. 5, No. 5. pp. 347-351.
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