Novel non-radioisotope immunoprecipitation studies indicate involvement of pemphigus vulgaris antigen in paraneoplastic pemphigus

T. Hashimoto, Masayuki Amagai, W. Ning, T. Nishikawa, T. Karashima, O. Mori, S. Jablonska, T. P. Chorzelski

Research output: Contribution to journalArticle

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Abstract

We have developed two different novel immunoprecipitation assays in which radioisotopes are not used, and have examined antigens for four cases of paraneoplastic pemphigus (PNP) including three new patients. The PNP sera showed a clear reactivity with transitional epithelia of rat urinary bladder by immunofluorescence, and reacted with a characteristic doublet of the 210 and 190 kD proteins by immunoblotting of normal human epidermal extract, confirming the diagnosis of PNP. In addition, by immunoprecipitation using silver-stain to detect immunoprecipitated proteins, the PNP sera detected the 250, 210 and 190 kD proteins, while control bullous pemphigoid sera detected only the 230 kD bullous pemphigoid antigen. Furthermore, with another immunoprecipitation using cell surface biotinylation, three of the four PNP sera specifically reacted with the 130 kD pemphigus vulgaris antigen (Dsg3), indicating that pemphigus vulgaris antigen may be involved in PNP. This reactivity was further suggested by the immunoblot analysis using recombinant pemphigus vulgaris antigen. In future, these non-radioisotope immunoprecipitation assays should become a useful tool not only to unravel the complex situation for the PNP antigens, but also to study antigens in other autoimmune bullous skin diseases.

Original languageEnglish
Pages (from-to)132-139
Number of pages8
JournalJournal of Dermatological Science
Volume17
Issue number2
DOIs
Publication statusPublished - 1998 Jun

Fingerprint

Pemphigus
Immunoprecipitation
Antigens
Bullous Pemphigoid
Assays
Serum
Proteins
Vesiculobullous Skin Diseases
Silver
Radioisotopes
Biotinylation
Rats
Skin
Coloring Agents
Immunoblotting
Fluorescent Antibody Technique
Urinary Bladder
Epithelium

Keywords

  • Autoantigen
  • Immunoblotting
  • Immunoprecipitation
  • Paraneoplastic pemphigus
  • Pemphigus vulgaris antigen

ASJC Scopus subject areas

  • Dermatology

Cite this

Novel non-radioisotope immunoprecipitation studies indicate involvement of pemphigus vulgaris antigen in paraneoplastic pemphigus. / Hashimoto, T.; Amagai, Masayuki; Ning, W.; Nishikawa, T.; Karashima, T.; Mori, O.; Jablonska, S.; Chorzelski, T. P.

In: Journal of Dermatological Science, Vol. 17, No. 2, 06.1998, p. 132-139.

Research output: Contribution to journalArticle

Hashimoto, T. ; Amagai, Masayuki ; Ning, W. ; Nishikawa, T. ; Karashima, T. ; Mori, O. ; Jablonska, S. ; Chorzelski, T. P. / Novel non-radioisotope immunoprecipitation studies indicate involvement of pemphigus vulgaris antigen in paraneoplastic pemphigus. In: Journal of Dermatological Science. 1998 ; Vol. 17, No. 2. pp. 132-139.
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