Novel non-thermal atrial fibrillation treatment with photosensitization reaction: Possibility of permanent electrical blockade in rat chronic model

Hiroki Matsuo, Arisa Ito, Shunichiro Miyoshi, Kyoko Soejima, Satoshi Ogawa, Tsunenori Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

We demonstrated a possibility of electrical conduction block by ex vivo and in vivo experiments using rat models to establish a non-thermal treatment for atrial fibrillation by photosensitization reaction (PR). One hour after the injection of 2 mg/kg Talaporfin sodium to Wistar rat, the right ventricle (1.4 mmT) was extracted. Paced with a stimulation electrode, this tissue was placed in a tissue bath and immersed in irrigated Tyrode's solution of 37°C with 8 μg/ml Talaporfin sodium and the gas mixture bubbling of 95% CO2 and 5% O2. The propagated electrical signal was measured by two bipolar electrodes. Exciting light of 670 nm in wavelength was irradiated to the tissue between the bipolar electrodes by the power density of 1 W/cm 2. After this irradiation, propagation signal blockade was obtained and continued up to three hours. Rat atrioventricular (AV) node was employed as a target region for chronic model. The heart of Wistar rat was surgically exposed. External four-lead electrocardiogram of this rat was measured. Thirty minutes after the injection of 10 mg/kg Talaporfin sodium to the rat, exciting light of 663 nm in wavelength was irradiated to the AV node by the power density of 500 mW/cm2 for ten minutes. Acute AV block was obtained during the irradiation. Two weeks after this procedure, complete AV block was confirmed. The rat was sacrificed to obtain the tissue specimen. We found that the AV node was replaced by scarring tissue under the microscopic observation of the specimen. We verified possibility of permanent electrical conduction block using PR.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7380
DOIs
Publication statusPublished - 2009
Event12th World Congress of the International Photodynamic Association - Photodynamic Therapy: Back to the Future - Seattle, WA, United States
Duration: 2009 Jun 112009 Jun 15

Other

Other12th World Congress of the International Photodynamic Association - Photodynamic Therapy: Back to the Future
CountryUnited States
CitySeattle, WA
Period09/6/1109/6/15

Fingerprint

fibrillation
Photosensitivity Disorders
Atrial Fibrillation
rats
Rats
Atrioventricular Node
Tissue
Electrodes
Atrioventricular Block
Wistar Rats
Sodium
sodium
Therapeutics
Light
electrodes
radiant flux density
Injections
Irradiation
Baths
injection

Keywords

  • atrial fibrillation
  • AV block
  • electrical blockade
  • photosensitization reaction
  • rat atrioventricular (AV) node
  • Talaporfin sodium

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Matsuo, H., Ito, A., Miyoshi, S., Soejima, K., Ogawa, S., & Arai, T. (2009). Novel non-thermal atrial fibrillation treatment with photosensitization reaction: Possibility of permanent electrical blockade in rat chronic model. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7380). [73805D] https://doi.org/10.1117/12.823074

Novel non-thermal atrial fibrillation treatment with photosensitization reaction : Possibility of permanent electrical blockade in rat chronic model. / Matsuo, Hiroki; Ito, Arisa; Miyoshi, Shunichiro; Soejima, Kyoko; Ogawa, Satoshi; Arai, Tsunenori.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7380 2009. 73805D.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Matsuo, H, Ito, A, Miyoshi, S, Soejima, K, Ogawa, S & Arai, T 2009, Novel non-thermal atrial fibrillation treatment with photosensitization reaction: Possibility of permanent electrical blockade in rat chronic model. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7380, 73805D, 12th World Congress of the International Photodynamic Association - Photodynamic Therapy: Back to the Future, Seattle, WA, United States, 09/6/11. https://doi.org/10.1117/12.823074
Matsuo H, Ito A, Miyoshi S, Soejima K, Ogawa S, Arai T. Novel non-thermal atrial fibrillation treatment with photosensitization reaction: Possibility of permanent electrical blockade in rat chronic model. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7380. 2009. 73805D https://doi.org/10.1117/12.823074
Matsuo, Hiroki ; Ito, Arisa ; Miyoshi, Shunichiro ; Soejima, Kyoko ; Ogawa, Satoshi ; Arai, Tsunenori. / Novel non-thermal atrial fibrillation treatment with photosensitization reaction : Possibility of permanent electrical blockade in rat chronic model. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7380 2009.
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