Novel screening method for agents that overcome classical multidrug resistance in a human cell line

Akihiko Yoshimura, N. Shudo, S. I. Ikeda, M. Ichikawa, T. Sumizawa, S. I. Akiyama

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

P-glycoprotein (P-gp) is involved in the transport of a wide variety of organic compounds including a fluorescent dye, rhodamine 6G (RG), as well as anti-cancer drugs. Agents that overcome classical multidrugresistance (MDR) increased the accumulation of RG in an MDR cell line, KB-C1. The effect of agents on RG-accumulation in KB-C1 cells was highly correlated with their effect on the reversion of vincristine-resistance in KB-C1 cells. RG was detected on a fluorescence microplate reader with a rhodamine channel. This assay may become a useful method for the screening of agents that overcome classical MBR, since it is quick and simple.

Original languageEnglish
Pages (from-to)45-51
Number of pages7
JournalCancer Letters
Volume50
Issue number1
DOIs
Publication statusPublished - 1990 Apr 9
Externally publishedYes

Fingerprint

KB Cells
Multiple Drug Resistance
Cell Line
Rhodamines
P-Glycoprotein
Vincristine
Fluorescent Dyes
Fluorescence
Pharmaceutical Preparations
Neoplasms
rhodamine 6G

Keywords

  • fluorescent dye
  • KB cell line
  • multidrug-resistance
  • resistance modifiers
  • screening method

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Novel screening method for agents that overcome classical multidrug resistance in a human cell line. / Yoshimura, Akihiko; Shudo, N.; Ikeda, S. I.; Ichikawa, M.; Sumizawa, T.; Akiyama, S. I.

In: Cancer Letters, Vol. 50, No. 1, 09.04.1990, p. 45-51.

Research output: Contribution to journalArticle

Yoshimura, Akihiko ; Shudo, N. ; Ikeda, S. I. ; Ichikawa, M. ; Sumizawa, T. ; Akiyama, S. I. / Novel screening method for agents that overcome classical multidrug resistance in a human cell line. In: Cancer Letters. 1990 ; Vol. 50, No. 1. pp. 45-51.
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