Novel screening method for agents that overcome classical multidrug resistance in a human cell line

A. Yoshimura, N. Shudo, S. I. Ikeda, M. Ichikawa, T. Sumizawa, S. I. Akiyama

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

P-glycoprotein (P-gp) is involved in the transport of a wide variety of organic compounds including a fluorescent dye, rhodamine 6G (RG), as well as anti-cancer drugs. Agents that overcome classical multidrugresistance (MDR) increased the accumulation of RG in an MDR cell line, KB-C1. The effect of agents on RG-accumulation in KB-C1 cells was highly correlated with their effect on the reversion of vincristine-resistance in KB-C1 cells. RG was detected on a fluorescence microplate reader with a rhodamine channel. This assay may become a useful method for the screening of agents that overcome classical MBR, since it is quick and simple.

Original languageEnglish
Pages (from-to)45-51
Number of pages7
JournalCancer Letters
Volume50
Issue number1
DOIs
Publication statusPublished - 1990 Apr 9
Externally publishedYes

Keywords

  • KB cell line
  • fluorescent dye
  • multidrug-resistance
  • resistance modifiers
  • screening method

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Novel screening method for agents that overcome classical multidrug resistance in a human cell line'. Together they form a unique fingerprint.

  • Cite this