Novel technique for peripheral nerve reconstruction in the absence of an artificial conduit

Takatsugu Komiyama, Yasushi Nakao, Yoshiaki Toyama, Charles A. Vacanti, Martin P. Vacanti, Ronald A. Ignotz

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The purpose of this study is to promote nerve regeneration across a peripheral nerve gap, using a biologic, tissue-engineered nerve (TEN), containing a high density of viable Schwann cells (SCs) in the absence of supportive foreign materials and a tubular system. Isolated SCs from adult rat sciatic nerve were seeded onto biodegradable constructs and implanted into the backs of nude mice to create TENs. Six weeks later, the constructs were harvested, implanted into surgically created sciatic nerve gaps in rats without supportive artificial conduits and compared with both an autograft group and a silicone conduit group using SCs. Two months later, functional assessment was evaluated by walking track analysis and the implanted lesions were imaged by transmission electron microscopy. The axonal number and sciatic function index of the TEN were significantly higher than those of the silicone group and achieved a comparable level to the autograft group. The results indicate that the large number of SCs within their own extracellular matrix appeared sufficient to enable neuronal growth across a nerve gap in the absence of an artificial conduit and that these circumstances may have a positive effect on the supplement of growth factors from the surrounding tissues of implanted TEN.

Original languageEnglish
Pages (from-to)133-140
Number of pages8
JournalJournal of Neuroscience Methods
Volume134
Issue number2
DOIs
Publication statusPublished - 2004 Apr 30

Fingerprint

Schwann Cells
Peripheral Nerves
Nerve Tissue
Autografts
Silicones
Sciatic Nerve
Nerve Regeneration
Transmission Electron Microscopy
Nude Mice
Walking
Extracellular Matrix
Intercellular Signaling Peptides and Proteins
Growth

Keywords

  • Artificial conduit
  • Pluronic F127
  • Polyglycolic acid
  • Schwann cell
  • Tissue engineering
  • Tissue-engineered nerve

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Komiyama, T., Nakao, Y., Toyama, Y., Vacanti, C. A., Vacanti, M. P., & Ignotz, R. A. (2004). Novel technique for peripheral nerve reconstruction in the absence of an artificial conduit. Journal of Neuroscience Methods, 134(2), 133-140. https://doi.org/10.1016/j.jneumeth.2003.11.020

Novel technique for peripheral nerve reconstruction in the absence of an artificial conduit. / Komiyama, Takatsugu; Nakao, Yasushi; Toyama, Yoshiaki; Vacanti, Charles A.; Vacanti, Martin P.; Ignotz, Ronald A.

In: Journal of Neuroscience Methods, Vol. 134, No. 2, 30.04.2004, p. 133-140.

Research output: Contribution to journalArticle

Komiyama, T, Nakao, Y, Toyama, Y, Vacanti, CA, Vacanti, MP & Ignotz, RA 2004, 'Novel technique for peripheral nerve reconstruction in the absence of an artificial conduit', Journal of Neuroscience Methods, vol. 134, no. 2, pp. 133-140. https://doi.org/10.1016/j.jneumeth.2003.11.020
Komiyama, Takatsugu ; Nakao, Yasushi ; Toyama, Yoshiaki ; Vacanti, Charles A. ; Vacanti, Martin P. ; Ignotz, Ronald A. / Novel technique for peripheral nerve reconstruction in the absence of an artificial conduit. In: Journal of Neuroscience Methods. 2004 ; Vol. 134, No. 2. pp. 133-140.
@article{d9561a6f3d434b03a5225f779e7def0d,
title = "Novel technique for peripheral nerve reconstruction in the absence of an artificial conduit",
abstract = "The purpose of this study is to promote nerve regeneration across a peripheral nerve gap, using a biologic, tissue-engineered nerve (TEN), containing a high density of viable Schwann cells (SCs) in the absence of supportive foreign materials and a tubular system. Isolated SCs from adult rat sciatic nerve were seeded onto biodegradable constructs and implanted into the backs of nude mice to create TENs. Six weeks later, the constructs were harvested, implanted into surgically created sciatic nerve gaps in rats without supportive artificial conduits and compared with both an autograft group and a silicone conduit group using SCs. Two months later, functional assessment was evaluated by walking track analysis and the implanted lesions were imaged by transmission electron microscopy. The axonal number and sciatic function index of the TEN were significantly higher than those of the silicone group and achieved a comparable level to the autograft group. The results indicate that the large number of SCs within their own extracellular matrix appeared sufficient to enable neuronal growth across a nerve gap in the absence of an artificial conduit and that these circumstances may have a positive effect on the supplement of growth factors from the surrounding tissues of implanted TEN.",
keywords = "Artificial conduit, Pluronic F127, Polyglycolic acid, Schwann cell, Tissue engineering, Tissue-engineered nerve",
author = "Takatsugu Komiyama and Yasushi Nakao and Yoshiaki Toyama and Vacanti, {Charles A.} and Vacanti, {Martin P.} and Ignotz, {Ronald A.}",
year = "2004",
month = "4",
day = "30",
doi = "10.1016/j.jneumeth.2003.11.020",
language = "English",
volume = "134",
pages = "133--140",
journal = "Journal of Neuroscience Methods",
issn = "0165-0270",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Novel technique for peripheral nerve reconstruction in the absence of an artificial conduit

AU - Komiyama, Takatsugu

AU - Nakao, Yasushi

AU - Toyama, Yoshiaki

AU - Vacanti, Charles A.

AU - Vacanti, Martin P.

AU - Ignotz, Ronald A.

PY - 2004/4/30

Y1 - 2004/4/30

N2 - The purpose of this study is to promote nerve regeneration across a peripheral nerve gap, using a biologic, tissue-engineered nerve (TEN), containing a high density of viable Schwann cells (SCs) in the absence of supportive foreign materials and a tubular system. Isolated SCs from adult rat sciatic nerve were seeded onto biodegradable constructs and implanted into the backs of nude mice to create TENs. Six weeks later, the constructs were harvested, implanted into surgically created sciatic nerve gaps in rats without supportive artificial conduits and compared with both an autograft group and a silicone conduit group using SCs. Two months later, functional assessment was evaluated by walking track analysis and the implanted lesions were imaged by transmission electron microscopy. The axonal number and sciatic function index of the TEN were significantly higher than those of the silicone group and achieved a comparable level to the autograft group. The results indicate that the large number of SCs within their own extracellular matrix appeared sufficient to enable neuronal growth across a nerve gap in the absence of an artificial conduit and that these circumstances may have a positive effect on the supplement of growth factors from the surrounding tissues of implanted TEN.

AB - The purpose of this study is to promote nerve regeneration across a peripheral nerve gap, using a biologic, tissue-engineered nerve (TEN), containing a high density of viable Schwann cells (SCs) in the absence of supportive foreign materials and a tubular system. Isolated SCs from adult rat sciatic nerve were seeded onto biodegradable constructs and implanted into the backs of nude mice to create TENs. Six weeks later, the constructs were harvested, implanted into surgically created sciatic nerve gaps in rats without supportive artificial conduits and compared with both an autograft group and a silicone conduit group using SCs. Two months later, functional assessment was evaluated by walking track analysis and the implanted lesions were imaged by transmission electron microscopy. The axonal number and sciatic function index of the TEN were significantly higher than those of the silicone group and achieved a comparable level to the autograft group. The results indicate that the large number of SCs within their own extracellular matrix appeared sufficient to enable neuronal growth across a nerve gap in the absence of an artificial conduit and that these circumstances may have a positive effect on the supplement of growth factors from the surrounding tissues of implanted TEN.

KW - Artificial conduit

KW - Pluronic F127

KW - Polyglycolic acid

KW - Schwann cell

KW - Tissue engineering

KW - Tissue-engineered nerve

UR - http://www.scopus.com/inward/record.url?scp=1542285375&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1542285375&partnerID=8YFLogxK

U2 - 10.1016/j.jneumeth.2003.11.020

DO - 10.1016/j.jneumeth.2003.11.020

M3 - Article

VL - 134

SP - 133

EP - 140

JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

SN - 0165-0270

IS - 2

ER -