Abstract
There is increasing evidence to suggest that the expression of many molecules in the lateral wall of the cochlea plays an important role in noise-induced stress responses. In this study, activation of the nuclear transcription factor nuclear factor-kappa B (NF-κB) was investigated in the cochlea of mice treated with intense noise exposure (4 kHz, octave band, 124 dB, for 2 h). The present noise exposure led to remarkable auditory brainstem response threshold shifts and cochlear damage on surface preparations. To assess the effects of noise exposure on NF-κB/DNA binding activity in the cochlea, we prepared nuclear extracts from the cochlea at different time points after noise exposure and carried out an electrophoretic mobility shift assay using a probe specific to NF-κB. NF-κB/DNA binding was significantly enhanced in the cochlea 2-6 h after noise exposure and returned to basal levels after 12 h. Supershift analysis using antibodies against p65 and p50 proteins, which are components of NF-κB, demonstrated that enhancement of NF-κB/DNA binding was at least in part due to nuclear translocation of p65. An immunohistochemical study also showed that nuclear translocation of both p65 and p50 was observed in the lateral wall after noise exposure and that there may be a possible close association between p65 and enhanced inducible nitric oxide synthase expression. These results suggest that NF-κB may have a detrimental role in the response to acoustic overstimulation in the cochlea of mice.
| Original language | English |
|---|---|
| Pages (from-to) | 237-247 |
| Number of pages | 11 |
| Journal | Brain Research |
| Volume | 1068 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2006 Jan 12 |
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Keywords
- Cochlea
- Inducible nitric oxide
- Lateral wall
- Noise trauma
- Nuclear factor-kappa B
- p65
ASJC Scopus subject areas
- Neuroscience(all)
- Clinical Neurology
- Developmental Biology
- Molecular Biology
Cite this
Nuclear factor-kappa B nuclear translocation in the cochlea of mice following acoustic overstimulation. / Masuda, Masatsugu; Nagashima, Reiko; Kanzaki, Sho; Fujioka, Masato; Ogita, Kiyokazu; Ogawa, Kaoru.
In: Brain Research, Vol. 1068, No. 1, 12.01.2006, p. 237-247.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nuclear factor-kappa B nuclear translocation in the cochlea of mice following acoustic overstimulation
AU - Masuda, Masatsugu
AU - Nagashima, Reiko
AU - Kanzaki, Sho
AU - Fujioka, Masato
AU - Ogita, Kiyokazu
AU - Ogawa, Kaoru
PY - 2006/1/12
Y1 - 2006/1/12
N2 - There is increasing evidence to suggest that the expression of many molecules in the lateral wall of the cochlea plays an important role in noise-induced stress responses. In this study, activation of the nuclear transcription factor nuclear factor-kappa B (NF-κB) was investigated in the cochlea of mice treated with intense noise exposure (4 kHz, octave band, 124 dB, for 2 h). The present noise exposure led to remarkable auditory brainstem response threshold shifts and cochlear damage on surface preparations. To assess the effects of noise exposure on NF-κB/DNA binding activity in the cochlea, we prepared nuclear extracts from the cochlea at different time points after noise exposure and carried out an electrophoretic mobility shift assay using a probe specific to NF-κB. NF-κB/DNA binding was significantly enhanced in the cochlea 2-6 h after noise exposure and returned to basal levels after 12 h. Supershift analysis using antibodies against p65 and p50 proteins, which are components of NF-κB, demonstrated that enhancement of NF-κB/DNA binding was at least in part due to nuclear translocation of p65. An immunohistochemical study also showed that nuclear translocation of both p65 and p50 was observed in the lateral wall after noise exposure and that there may be a possible close association between p65 and enhanced inducible nitric oxide synthase expression. These results suggest that NF-κB may have a detrimental role in the response to acoustic overstimulation in the cochlea of mice.
AB - There is increasing evidence to suggest that the expression of many molecules in the lateral wall of the cochlea plays an important role in noise-induced stress responses. In this study, activation of the nuclear transcription factor nuclear factor-kappa B (NF-κB) was investigated in the cochlea of mice treated with intense noise exposure (4 kHz, octave band, 124 dB, for 2 h). The present noise exposure led to remarkable auditory brainstem response threshold shifts and cochlear damage on surface preparations. To assess the effects of noise exposure on NF-κB/DNA binding activity in the cochlea, we prepared nuclear extracts from the cochlea at different time points after noise exposure and carried out an electrophoretic mobility shift assay using a probe specific to NF-κB. NF-κB/DNA binding was significantly enhanced in the cochlea 2-6 h after noise exposure and returned to basal levels after 12 h. Supershift analysis using antibodies against p65 and p50 proteins, which are components of NF-κB, demonstrated that enhancement of NF-κB/DNA binding was at least in part due to nuclear translocation of p65. An immunohistochemical study also showed that nuclear translocation of both p65 and p50 was observed in the lateral wall after noise exposure and that there may be a possible close association between p65 and enhanced inducible nitric oxide synthase expression. These results suggest that NF-κB may have a detrimental role in the response to acoustic overstimulation in the cochlea of mice.
KW - Cochlea
KW - Inducible nitric oxide
KW - Lateral wall
KW - Noise trauma
KW - Nuclear factor-kappa B
KW - p65
UR - http://www.scopus.com/inward/record.url?scp=31344438668&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=31344438668&partnerID=8YFLogxK
U2 - 10.1016/j.brainres.2005.11.020
DO - 10.1016/j.brainres.2005.11.020
M3 - Article
C2 - 16376312
AN - SCOPUS:31344438668
VL - 1068
SP - 237
EP - 247
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1
ER -