Nuclear magnetic resonance analysis of the conformational state of cancer mutant of fibroblast growth factor receptor 1 tyrosine kinase domain

Yoshihiro Kobashigawa, Shinjiro Amano, Kaito Yoza, Rika Himeno, Shun Amemiya, Hiroshi Morioka, Mariko Yokogawa, Hiroyuki Kumeta, Joseph Schlessinger, Fuyuhiko Inagaki

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Tyrosine kinases are key enzymes that play critical roles in growth signaling, the abnormal activation of which is associated with various human cancers. Activation of tyrosine kinases is mediated by tyrosine phosphorylation in the activation-loop, which transforms the catalytic domain to the active state conformation. Cancer mutations are supposed to transform the conformation of the catalytic domain into the active-form independent of the phosphorylation state of the activation-loop. Here, we report structural and biophysical analyses of cancer mutations of the tyrosine kinase domain of fibroblast growth factor receptor 1 (FGFR1). Based on the nuclear magnetic resonance analyses, phosphorylation of the activation-loop exhibited cooperative structural transition in the activation-loop, C-helix and P-loop regions, whereas cancer mutations induced structural transformation at either one or two of these regions.

Original languageEnglish
JournalGenes to Cells
DOIs
Publication statusAccepted/In press - 2016
Externally publishedYes

Fingerprint

Receptor, Fibroblast Growth Factor, Type 1
Protein-Tyrosine Kinases
Magnetic Resonance Spectroscopy
Phosphorylation
Mutation
Catalytic Domain
Neoplasms
Activation Analysis
Tyrosine
Enzymes
Growth

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Nuclear magnetic resonance analysis of the conformational state of cancer mutant of fibroblast growth factor receptor 1 tyrosine kinase domain. / Kobashigawa, Yoshihiro; Amano, Shinjiro; Yoza, Kaito; Himeno, Rika; Amemiya, Shun; Morioka, Hiroshi; Yokogawa, Mariko; Kumeta, Hiroyuki; Schlessinger, Joseph; Inagaki, Fuyuhiko.

In: Genes to Cells, 2016.

Research output: Contribution to journalArticle

Kobashigawa, Yoshihiro ; Amano, Shinjiro ; Yoza, Kaito ; Himeno, Rika ; Amemiya, Shun ; Morioka, Hiroshi ; Yokogawa, Mariko ; Kumeta, Hiroyuki ; Schlessinger, Joseph ; Inagaki, Fuyuhiko. / Nuclear magnetic resonance analysis of the conformational state of cancer mutant of fibroblast growth factor receptor 1 tyrosine kinase domain. In: Genes to Cells. 2016.
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AU - Amano, Shinjiro

AU - Yoza, Kaito

AU - Himeno, Rika

AU - Amemiya, Shun

AU - Morioka, Hiroshi

AU - Yokogawa, Mariko

AU - Kumeta, Hiroyuki

AU - Schlessinger, Joseph

AU - Inagaki, Fuyuhiko

PY - 2016

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