Nuclear magnetic resonance approaches for characterizing protein-protein interactions

Yuki Toyama, Yoko Mase, Hanaho Kano, Mariko Yokogawa, Masanori Osawa, Ichio Shimada

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The gating of potassium ion (K+) channels is regulated by various kinds of protein-protein interactions (PPIs). Structural investigations of these PPIs provide useful information not only for understanding the gating mechanisms of K+ channels, but also for developing the pharmaceutical compounds targeting K+ channels. Here, we describe a nuclear magnetic resonance spectroscopic method, termed the cross saturation (CS) method, to accurately determine the binding surfaces of protein complexes, and its application to the investigation of the interaction between a G protein-coupled inwardly rectifying K+ channel and a G protein α subunit.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages115-128
Number of pages14
Volume1684
DOIs
Publication statusPublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1684
ISSN (Print)1064-3745

Fingerprint

Magnetic Resonance Spectroscopy
GTP-Binding Proteins
Proteins
Inwardly Rectifying Potassium Channel
Potassium Channels
Protein Subunits
Carrier Proteins
Membrane Proteins
Pharmaceutical Preparations

Keywords

  • Cross saturation
  • G protein
  • GIRK
  • NMR
  • Protein-protein interaction

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Toyama, Y., Mase, Y., Kano, H., Yokogawa, M., Osawa, M., & Shimada, I. (2018). Nuclear magnetic resonance approaches for characterizing protein-protein interactions. In Methods in Molecular Biology (Vol. 1684, pp. 115-128). (Methods in Molecular Biology; Vol. 1684). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7362-0_10

Nuclear magnetic resonance approaches for characterizing protein-protein interactions. / Toyama, Yuki; Mase, Yoko; Kano, Hanaho; Yokogawa, Mariko; Osawa, Masanori; Shimada, Ichio.

Methods in Molecular Biology. Vol. 1684 Humana Press Inc., 2018. p. 115-128 (Methods in Molecular Biology; Vol. 1684).

Research output: Chapter in Book/Report/Conference proceedingChapter

Toyama, Y, Mase, Y, Kano, H, Yokogawa, M, Osawa, M & Shimada, I 2018, Nuclear magnetic resonance approaches for characterizing protein-protein interactions. in Methods in Molecular Biology. vol. 1684, Methods in Molecular Biology, vol. 1684, Humana Press Inc., pp. 115-128. https://doi.org/10.1007/978-1-4939-7362-0_10
Toyama Y, Mase Y, Kano H, Yokogawa M, Osawa M, Shimada I. Nuclear magnetic resonance approaches for characterizing protein-protein interactions. In Methods in Molecular Biology. Vol. 1684. Humana Press Inc. 2018. p. 115-128. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7362-0_10
Toyama, Yuki ; Mase, Yoko ; Kano, Hanaho ; Yokogawa, Mariko ; Osawa, Masanori ; Shimada, Ichio. / Nuclear magnetic resonance approaches for characterizing protein-protein interactions. Methods in Molecular Biology. Vol. 1684 Humana Press Inc., 2018. pp. 115-128 (Methods in Molecular Biology).
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