Nuclear translocation of Xenopus laevis paxillin

Motoyuki Ogawa, Yoshiki Hiraoka, Sadakazu Aiso

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Paxillin has been recognized as a focal adhesion adapter protein that participates in the integrin-mediated signaling. An earlier study [Ogawa et al. Biochim. Biophys. Acta 1519 (2001) 235] found that frog paxillin was expressed in the kidney epithelial cell line A6 and localized in the nucleus. Here, in this study, we have found that the expression of frog paxillin is up-regulated in the S phase of cell cycle. The protein became phosphorylated on tyrosine when the cells were grown on vitronectin; the tyrosine phosphorylation was not detectable when the cells were cultured on fibronectin, laminin or poly-D-lysine. On the other hand, MAP kinase was revealed to phosphorylate frog paxillin on serine. Both phosphorylation events, namely on tyrosine and serine, were essential for the nuclear translocation of this protein. Our results suggest that the integrin-mediated signaling pathway and the MAP kinase pathway meet at paxillin.

Original languageEnglish
Pages (from-to)676-683
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume304
Issue number4
DOIs
Publication statusPublished - 2003 May 16

Fingerprint

Paxillin
Xenopus laevis
Anura
Tyrosine
Phosphorylation
Integrins
Serine
Phosphotransferases
Vitronectin
Proteins
Focal Adhesions
MAP Kinase Signaling System
Laminin
Nuclear Proteins
S Phase
Fibronectins
Lysine
Cultured Cells
Cell Cycle
Adhesion

Keywords

  • Cell adhesion
  • Cell cycle
  • Extracellular matrix
  • Nuclear translocation
  • Paxillin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Nuclear translocation of Xenopus laevis paxillin. / Ogawa, Motoyuki; Hiraoka, Yoshiki; Aiso, Sadakazu.

In: Biochemical and Biophysical Research Communications, Vol. 304, No. 4, 16.05.2003, p. 676-683.

Research output: Contribution to journalArticle

Ogawa, Motoyuki ; Hiraoka, Yoshiki ; Aiso, Sadakazu. / Nuclear translocation of Xenopus laevis paxillin. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 304, No. 4. pp. 676-683.
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