Nucleolar targeting signal of human T-cell leukemia virus type I rex-encoded protein is essential for cytoplasmic accumulation of unspliced viral mRNA

T. Nosaka, H. Siomi, Y. Adachi, M. Ishibashi, S. Kubota, M. Maki, M. Hatanaka

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

The posttranscriptional regulator (rex) of human T-cell leukemia virus type I is known to be located predominantly in the cell nucleolus and to induce the accumulation of gag and env viral mRNAs. The N-terminal 19 amino acids of rex-encoded protein (Rex) has been shown to be sufficient to direct hybrid proteins to the cell nucleolus. We have studied the function of the nucleolar targeting signal (NOS) of rex by using full-length proviral DNA and mutant rex expression plasmids. Partial deletions of the NOS sequence abolished the accumulation of unspliced cytoplasmic mRNA, although the gene products of rex mutants were found in the nucleoplasm. These results indicate that NOS sequence, or nucleolar localization of Rex, is essential for Rex function.

Original languageEnglish
Pages (from-to)9798-9802
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number24
DOIs
Publication statusPublished - 1989

Keywords

  • mRNA export
  • nucleolus
  • rex gene function

ASJC Scopus subject areas

  • General

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