Nucleotide sequence and analysis of the phoB-rrnE-groESL region of the Bacillus subtilis chromosome

Yoshito Sadaie, Katsunori Yata, Masaya Fujita, Hitoshi Sagai, Mitsuhiro Itaya, Yasuhiro Kasahara, Naotake Ogasawara

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A 36 kb sequence of the phoB-rrnE-groESL region of the Bacillus subtilis chromosome at around 55°has been determined. The sequenced region contains 36 ORFs including the phoB and groESL genes, and the whole rrnE operon. The phoB gene is transcribed in the direction opposite to that of chromosome replication, while most ORFs, including groESL and the rrnE operon, are transcribed in the same direction. Two newly identified tRNA genes upstream of the rrnE operon were those for Arg-tRNA and Gly-tRNA. The sequenced region contains an operon consisting of genes for degradation and uptake of mannan. The rrnE operon and its downstream ORFs are well conserved among Mycoplasma genitalium, Haemophilus influenzae, Synechocystis sp. and Methanococcus jannaschii. σ(H) consensus sequences are present in the promoter regions of three ORFs, including groESL.

Original languageEnglish
Pages (from-to)1861-1866
Number of pages6
JournalMicrobiology
Volume143
Issue number6
Publication statusPublished - 1997 Jun
Externally publishedYes

Fingerprint

Operon
Bacillus subtilis
Sequence Analysis
Chromosomes
Open Reading Frames
Transfer RNA
Genes
RNA, Transfer, Gly
Mycoplasma genitalium
Methanocaldococcus
Synechocystis
Mannans
Consensus Sequence
Haemophilus influenzae
Genetic Promoter Regions
Direction compound

Keywords

  • Bacillus subtilis
  • Genome sequencing
  • groESL
  • phoB
  • rrnE

ASJC Scopus subject areas

  • Microbiology

Cite this

Sadaie, Y., Yata, K., Fujita, M., Sagai, H., Itaya, M., Kasahara, Y., & Ogasawara, N. (1997). Nucleotide sequence and analysis of the phoB-rrnE-groESL region of the Bacillus subtilis chromosome. Microbiology, 143(6), 1861-1866.

Nucleotide sequence and analysis of the phoB-rrnE-groESL region of the Bacillus subtilis chromosome. / Sadaie, Yoshito; Yata, Katsunori; Fujita, Masaya; Sagai, Hitoshi; Itaya, Mitsuhiro; Kasahara, Yasuhiro; Ogasawara, Naotake.

In: Microbiology, Vol. 143, No. 6, 06.1997, p. 1861-1866.

Research output: Contribution to journalArticle

Sadaie, Y, Yata, K, Fujita, M, Sagai, H, Itaya, M, Kasahara, Y & Ogasawara, N 1997, 'Nucleotide sequence and analysis of the phoB-rrnE-groESL region of the Bacillus subtilis chromosome', Microbiology, vol. 143, no. 6, pp. 1861-1866.
Sadaie Y, Yata K, Fujita M, Sagai H, Itaya M, Kasahara Y et al. Nucleotide sequence and analysis of the phoB-rrnE-groESL region of the Bacillus subtilis chromosome. Microbiology. 1997 Jun;143(6):1861-1866.
Sadaie, Yoshito ; Yata, Katsunori ; Fujita, Masaya ; Sagai, Hitoshi ; Itaya, Mitsuhiro ; Kasahara, Yasuhiro ; Ogasawara, Naotake. / Nucleotide sequence and analysis of the phoB-rrnE-groESL region of the Bacillus subtilis chromosome. In: Microbiology. 1997 ; Vol. 143, No. 6. pp. 1861-1866.
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