Nucleotide sequence and structure of the mouse cytokeratin endoB gene

Icliinose Yuki, Morita Takashi, Zhang Fengyun, Srimahasongcram Sattaporn, Maria Lucia C Tondella, Midori Matsumoto, Nozaki Masami, Matsushiro Aizo

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We report the isolation and nucleotide sequence (7878 bp) of a genomic clone encoding murine cytokeratin EndoB, a type-I intermediate filament protein that is expressed coordinately with EndoA in trophectoderm cells during blastocyst formation. This clone contains the complete endoB gene including the 5' upstream and 3' downstream flanking regions. Sequencing analysis reveals the endoB gene consists of seven exons and six introns. Although the positions of the six introns coincide exactly with those of other type-I cytokeratin genes, the endoB gene has lost the seventh intron, which resides in the sequences coding for tail domains of other type-I cytokeratins. The 5' upstream regions of endoA and endoB genes contain homologous sequences around the respective TATA-like boxes, suggesting their involvement in coordinate regulation of their transcription and/or post-transcription. Surprisingly, this clone contains at least four murine B l repetitive sequences. One typical B1 repetitive sequence was recognized in the 5' upstream region, and the other three in the 3' flanking region.

Original languageEnglish
Pages (from-to)85-95
Number of pages11
JournalGene
Volume70
Issue number1
DOIs
Publication statusPublished - 1988 Oct 15
Externally publishedYes

Fingerprint

Keratins
Introns
3' Flanking Region
Clone Cells
Nucleic Acid Repetitive Sequences
Genes
Type I Keratin
TATA Box
Blastocyst
Sequence Homology
Tail
Exons

Keywords

  • Bl repetitive sequence
  • exon-intron pattern
  • gene family
  • gene structure
  • intermediate filament
  • mouse embryo
  • Recombinant DNA
  • teratocarcinoma

ASJC Scopus subject areas

  • Genetics

Cite this

Yuki, I., Takashi, M., Fengyun, Z., Sattaporn, S., Tondella, M. L. C., Matsumoto, M., ... Aizo, M. (1988). Nucleotide sequence and structure of the mouse cytokeratin endoB gene. Gene, 70(1), 85-95. https://doi.org/10.1016/0378-1119(88)90107-2

Nucleotide sequence and structure of the mouse cytokeratin endoB gene. / Yuki, Icliinose; Takashi, Morita; Fengyun, Zhang; Sattaporn, Srimahasongcram; Tondella, Maria Lucia C; Matsumoto, Midori; Masami, Nozaki; Aizo, Matsushiro.

In: Gene, Vol. 70, No. 1, 15.10.1988, p. 85-95.

Research output: Contribution to journalArticle

Yuki, I, Takashi, M, Fengyun, Z, Sattaporn, S, Tondella, MLC, Matsumoto, M, Masami, N & Aizo, M 1988, 'Nucleotide sequence and structure of the mouse cytokeratin endoB gene', Gene, vol. 70, no. 1, pp. 85-95. https://doi.org/10.1016/0378-1119(88)90107-2
Yuki I, Takashi M, Fengyun Z, Sattaporn S, Tondella MLC, Matsumoto M et al. Nucleotide sequence and structure of the mouse cytokeratin endoB gene. Gene. 1988 Oct 15;70(1):85-95. https://doi.org/10.1016/0378-1119(88)90107-2
Yuki, Icliinose ; Takashi, Morita ; Fengyun, Zhang ; Sattaporn, Srimahasongcram ; Tondella, Maria Lucia C ; Matsumoto, Midori ; Masami, Nozaki ; Aizo, Matsushiro. / Nucleotide sequence and structure of the mouse cytokeratin endoB gene. In: Gene. 1988 ; Vol. 70, No. 1. pp. 85-95.
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