Nucleotides bearing a cleavable genotoxic group on the phosphate.

H. Hayatsu, M. Ishii, M. Akashi, N. Inada, S. Takashima, Satoko Ishikawa, S. Hizatate, M. Mochizuki

Research output: Contribution to journalArticle

Abstract

A new class of nucleotide derivatives in which N-nitroso-pyrrolidine (NPYR) is linked to the phosphate group through an ester linkage at the alpha-carbon of the nitrosamine were prepared by a reaction between a nucleoside monophosphate and alpha-acetoxy-NPYR. The NPYR derivatives were prepared from dpT, dpC, dpA, dpG, pA and dpCpT. Treatment of the NPYR-pX with acid, snake venom phosphodiesterase, or near ultraviolet light caused cleavage of the NPYR group, regenerating pX. All of these nucleotide derivatives were directly mutagenic towards Salmonella typhimurium TA1535. When phage M13mp2 double-stranded covalently closed circular DNA was treated with NPYR-dpT with near ultraviolet irradiation, single strand breaks of the DNA took place.

Original languageEnglish
Pages (from-to)217-218
Number of pages2
JournalNucleic acids symposium series
Issue number29
Publication statusPublished - 1993
Externally publishedYes

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Nucleotides
Phosphates
Single-Stranded DNA Breaks
Circular DNA
Nitrosamines
Salmonella typhimurium
Ultraviolet Rays
Nucleosides
Bacteriophages
pyrrolidine
Esters
Carbon
Acids

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Hayatsu, H., Ishii, M., Akashi, M., Inada, N., Takashima, S., Ishikawa, S., ... Mochizuki, M. (1993). Nucleotides bearing a cleavable genotoxic group on the phosphate. Nucleic acids symposium series, (29), 217-218.

Nucleotides bearing a cleavable genotoxic group on the phosphate. / Hayatsu, H.; Ishii, M.; Akashi, M.; Inada, N.; Takashima, S.; Ishikawa, Satoko; Hizatate, S.; Mochizuki, M.

In: Nucleic acids symposium series, No. 29, 1993, p. 217-218.

Research output: Contribution to journalArticle

Hayatsu, H, Ishii, M, Akashi, M, Inada, N, Takashima, S, Ishikawa, S, Hizatate, S & Mochizuki, M 1993, 'Nucleotides bearing a cleavable genotoxic group on the phosphate.', Nucleic acids symposium series, no. 29, pp. 217-218.
Hayatsu H, Ishii M, Akashi M, Inada N, Takashima S, Ishikawa S et al. Nucleotides bearing a cleavable genotoxic group on the phosphate. Nucleic acids symposium series. 1993;(29):217-218.
Hayatsu, H. ; Ishii, M. ; Akashi, M. ; Inada, N. ; Takashima, S. ; Ishikawa, Satoko ; Hizatate, S. ; Mochizuki, M. / Nucleotides bearing a cleavable genotoxic group on the phosphate. In: Nucleic acids symposium series. 1993 ; No. 29. pp. 217-218.
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