Numerical simulation of near-field fluorescence correlation spectroscopy using a fiber probe

Kazuo Kasahara; Toshiharu Saiki

doi:10.1117/1.3319569

Numerical simulation of near-field fluorescence correlation spectroscopy using a fiber probe

Kazuo Kasahara, Toshiharu Saiki

Department of Electronics and Electrical Engineering

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Numerical simulation of fluorescence correlation spectroscopy (FCS) based on near-field scanning optical microscopy (NSOM) was performed. A finite-difference time-domain method was employed to calculate the electric field distribution in the vicinity of the NSOM aperture and the Brownian motion of nanoparticles was reproduced by a Monte Carlo simulation. The physical validity of the simulation result was confirmed by comparing it with a theoretical calculation using a Gaussian observation volume, which is applied to FCS based on conventional optical microscopy.

Original language	English
Article number	043502
Journal	Journal of Nanophotonics
Volume	4
Issue number	1
DOIs	https://doi.org/10.1117/1.3319569
Publication status	Published - 2010

Keywords

Brownian motion
Fluorescence spectroscopy
Near-field scanning optical microscopy
Optical correlation

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics

Access to Document

10.1117/1.3319569

Cite this

@article{0686dfcbe0d344ad86a316fd038ccca9,

title = "Numerical simulation of near-field fluorescence correlation spectroscopy using a fiber probe",

abstract = "Numerical simulation of fluorescence correlation spectroscopy (FCS) based on near-field scanning optical microscopy (NSOM) was performed. A finite-difference time-domain method was employed to calculate the electric field distribution in the vicinity of the NSOM aperture and the Brownian motion of nanoparticles was reproduced by a Monte Carlo simulation. The physical validity of the simulation result was confirmed by comparing it with a theoretical calculation using a Gaussian observation volume, which is applied to FCS based on conventional optical microscopy.",

keywords = "Brownian motion, Fluorescence spectroscopy, Near-field scanning optical microscopy, Optical correlation",

author = "Kazuo Kasahara and Toshiharu Saiki",

note = "Funding Information: This work was supported in part by a Grant-in-Aid from the Global Center of Excellence for High-Level Global Cooperation for Leading-Edge Platform on Access Spaces and a Grant-in-Aid for Scientific Research (S) from the Ministry of Education, Culture, Sport, Science, and Technology in Japan.",

year = "2010",

doi = "10.1117/1.3319569",

language = "English",

volume = "4",

journal = "Journal of Nanophotonics",

issn = "1934-2608",

publisher = "SPIE",

number = "1",

}

TY - JOUR

T1 - Numerical simulation of near-field fluorescence correlation spectroscopy using a fiber probe

AU - Kasahara, Kazuo

AU - Saiki, Toshiharu

N1 - Funding Information: This work was supported in part by a Grant-in-Aid from the Global Center of Excellence for High-Level Global Cooperation for Leading-Edge Platform on Access Spaces and a Grant-in-Aid for Scientific Research (S) from the Ministry of Education, Culture, Sport, Science, and Technology in Japan.

PY - 2010

Y1 - 2010

N2 - Numerical simulation of fluorescence correlation spectroscopy (FCS) based on near-field scanning optical microscopy (NSOM) was performed. A finite-difference time-domain method was employed to calculate the electric field distribution in the vicinity of the NSOM aperture and the Brownian motion of nanoparticles was reproduced by a Monte Carlo simulation. The physical validity of the simulation result was confirmed by comparing it with a theoretical calculation using a Gaussian observation volume, which is applied to FCS based on conventional optical microscopy.

AB - Numerical simulation of fluorescence correlation spectroscopy (FCS) based on near-field scanning optical microscopy (NSOM) was performed. A finite-difference time-domain method was employed to calculate the electric field distribution in the vicinity of the NSOM aperture and the Brownian motion of nanoparticles was reproduced by a Monte Carlo simulation. The physical validity of the simulation result was confirmed by comparing it with a theoretical calculation using a Gaussian observation volume, which is applied to FCS based on conventional optical microscopy.

KW - Brownian motion

KW - Fluorescence spectroscopy

KW - Near-field scanning optical microscopy

KW - Optical correlation

UR - http://www.scopus.com/inward/record.url?scp=76249128050&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=76249128050&partnerID=8YFLogxK

U2 - 10.1117/1.3319569

DO - 10.1117/1.3319569

M3 - Article

AN - SCOPUS:76249128050

SN - 1934-2608

VL - 4

JO - Journal of Nanophotonics

JF - Journal of Nanophotonics

IS - 1

M1 - 043502

ER -

Numerical simulation of near-field fluorescence correlation spectroscopy using a fiber probe

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this