Numerical simulation of fluorescence correlation spectroscopy (FCS) based on near-field scanning optical microscopy (NSOM) was performed. A finite-difference time-domain method was employed to calculate the electric field distribution in the vicinity of the NSOM aperture and the Brownian motion of nanoparticles was reproduced by a Monte Carlo simulation. The physical validity of the simulation result was confirmed by comparing it with a theoretical calculation using a Gaussian observation volume, which is applied to FCS based on conventional optical microscopy.
- Brownian motion
- Fluorescence spectroscopy
- Near-field scanning optical microscopy
- Optical correlation
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics