To observe living cell morphology on ceramics by light microscopy, we fabricated a new material - transparent β-tricalcium phosphate (t-βTCP) ceramic - for the purpose of serving as a tissue culture substrate. Bone marrow stromal cells (BMSCs) were obtained from rat femora and cultured on both t-βTCP ceramic disks and culture grade polystyrene (PS) dishes in an osteogenic medium. After 1 day of culture, cell attachment and spreading on both the t-βTCP and PS substrata were equally and clearly detected by ordinary light microscopy. After 14 days of culture, extensive cell growth, alkaline phosphatase (ALP) staining, and bone mineral deposition could be detected on both substrata. In addition, quantitative biochemical analyses revealed high DNA content, ALP activity, and osteocalcin content of these cultures. This experiment is significant in that all of the results were similarly observed on both the t-βTCP and PS substrata, indicating the excellent properties of βTCP ceramics for BMSCs culture towards osteogenic differentiation.
|Number of pages||9|
|Journal||Journal of Materials Science: Materials in Medicine|
|Publication status||Published - 2006 Jan 1|
ASJC Scopus subject areas
- Biomedical Engineering