Observation of subcellular distribution of antisense oligonucleotide/lipid complexes by confocal laser scanning microscope

Toshinori Sato, Hirotaka Nishi, Hiroyuki Akino, Yoshio Okahata, Yoko Shoji, Jingoro Shimada

Research output: Contribution to journalArticle

Abstract

Distribution of phosphorothioate antisense oligonucleotide (S-oligo), S-oligo/lipofectin complex and S-oligo/PEG lipid complex in Hela cells was observed by a confocal laser scanning microscope. The S-oligo localized to endosome/lysosome in a punctate fluorescence pattern. In the presence of lipofectin, the S-oligo was accumulated in the nucleus of Hela cells. The S-oligo/PEG lipid complex showed both punctate fluorescence and diffuse fluorescence in the cyoplasm, but not in the nucleus. These result clearly demonstrated that the subcellular distribution of S-oligo depended on the delivery system.

Original languageEnglish
Pages (from-to)359-364
Number of pages6
JournalDrug Delivery System
Volume13
Issue number5
DOIs
Publication statusPublished - 1998
Externally publishedYes

Keywords

  • antisense oligonucleotide
  • DNA/lipid complex
  • subcellular distribution

ASJC Scopus subject areas

  • Pharmaceutical Science

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