Observation of subcellular distribution of antisense oligonucleotide/lipid complexes by confocal laser scanning microscope

Toshinori Sato, Hirotaka Nishi, Hiroyuki Akino, Yoshio Okahata, Yoko Shoji, Jingoro Shimada

Research output: Contribution to journalArticle

Abstract

Distribution of phosphorothioate antisense oligonucleotide (S-oligo), S-oligo/lipofectin complex and S-oligo/PEG lipid complex in Hela cells was observed by a confocal laser scanning microscope. The S-oligo localized to endosome/lysosome in a punctate fluorescence pattern. In the presence of lipofectin, the S-oligo was accumulated in the nucleus of Hela cells. The S-oligo/PEG lipid complex showed both punctate fluorescence and diffuse fluorescence in the cyoplasm, but not in the nucleus. These result clearly demonstrated that the subcellular distribution of S-oligo depended on the delivery system.

Original languageEnglish
Pages (from-to)359-364
Number of pages6
JournalDrug Delivery System
Volume13
Issue number5
DOIs
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Antisense Oligonucleotides
Lasers
Fluorescence
Observation
Lipids
HeLa Cells
Phosphorothioate Oligonucleotides
Endosomes
Lysosomes
1,2-dielaidoylphosphatidylethanolamine

Keywords

  • antisense oligonucleotide
  • DNA/lipid complex
  • subcellular distribution

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Observation of subcellular distribution of antisense oligonucleotide/lipid complexes by confocal laser scanning microscope. / Sato, Toshinori; Nishi, Hirotaka; Akino, Hiroyuki; Okahata, Yoshio; Shoji, Yoko; Shimada, Jingoro.

In: Drug Delivery System, Vol. 13, No. 5, 1998, p. 359-364.

Research output: Contribution to journalArticle

Sato, Toshinori ; Nishi, Hirotaka ; Akino, Hiroyuki ; Okahata, Yoshio ; Shoji, Yoko ; Shimada, Jingoro. / Observation of subcellular distribution of antisense oligonucleotide/lipid complexes by confocal laser scanning microscope. In: Drug Delivery System. 1998 ; Vol. 13, No. 5. pp. 359-364.
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