Observations of the distribution of GM3 in membrane microdomains by atomic force microscopy

Kazutoshi Iijima, Norihiro Soga, Teruhiko Matsubara, Toshinori Sato

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Glycosphingolipids (GSLs) in membrane microdomains participate in important biological functions. In the present paper, we propose a novel model of the distribution of GSLs in membrane microdomains composed of sphingomyelin (SM) and cholesterol. We investigated the distribution of the ganglioside GM3 in a lipid membrane reconstituted with lipid extract from mouse B16 melanoma cells using an atomic force microscope (AFM). The surface topography of the reconstituted lipid bilayer showed three areas of different heights. The highest area was confirmed to be a GM3 domain by labeling with wheat germ agglutinin. To identify the lipids which are contributed to make the topography, the topographies of the artificial lipid bilayers composed of GM3, SM, 1-palmitoyl-2-oleoyl-phosphatidylcholine, and cholesterol were investigated. AFM images of the artificial lipid bilayers showed that the GM3 domain surrounded by a SM-containing phase only occurred, and its formation was found to depend on the cholesterol content.

Original languageEnglish
Pages (from-to)369-374
Number of pages6
JournalJournal of Colloid and Interface Science
Volume337
Issue number2
DOIs
Publication statusPublished - 2009 Sep 15

Fingerprint

Lipid bilayers
Sphingomyelins
Cholesterol
Atomic force microscopy
Glycosphingolipids
Membranes
Topography
Lipids
Microscopes
G(M3) Ganglioside
Wheat Germ Agglutinins
Surface topography
Membrane Lipids
Labeling

Keywords

  • Atomic force microscope
  • Glycosphingolipid
  • Lipid raft
  • Membrane microdomain

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Colloid and Surface Chemistry

Cite this

Observations of the distribution of GM3 in membrane microdomains by atomic force microscopy. / Iijima, Kazutoshi; Soga, Norihiro; Matsubara, Teruhiko; Sato, Toshinori.

In: Journal of Colloid and Interface Science, Vol. 337, No. 2, 15.09.2009, p. 369-374.

Research output: Contribution to journalArticle

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