Occludin as a functional marker of vascular endothelial cells on tube-forming activity

Toshie Kanayasu-Toyoda, Akiko Ishii-Watabe, Yutaka Kikuchi, Hiroko Kitagawa, Hiroko Suzuki, Hiroomi Tamura, Minoru Tada, Takuo Suzuki, Hiroyuki Mizuguchi, Teruhide Yamaguchi

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Abstract

Cell therapy using endothelial progenitor cells (EPCs) is a promising strategy for the treatment of ischemic diseases. Two types of EPCs have been identified: early EPCs and late EPCs. Late EPCs are able to form tube structure by themselves, and have a high proliferative ability. The functional marker(s) of late EPCs, which relate to their therapeutic potential, have not been fully elucidated. Here we compared the gene expression profiles of several human cord blood derived late EPC lines which exhibit different tube formation activity, and we observed that the expression of occludin (OCLN) in these lines correlated with the tube formation ability, suggesting that OCLN is a candidate functional marker of late EPCs. When OCLN was knocked down by transfecting siRNA, the tube formation on Matrigel, the S phase + G2/M phase in the cell cycle, and the spheroid-based sprouting of late EPCs were markedly reduced, suggesting the critical role of OCLN in tube formation, sprouting, and proliferation. These results indicated that OCLN plays a novel role in neovascularization and angiogenesis.

Original languageEnglish
Pages (from-to)1700-1711
Number of pages12
JournalJournal of Cellular Physiology
Volume233
Issue number2
DOIs
Publication statusPublished - 2018 Feb 1

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Keywords

  • late endothelial progenitor cell
  • occludin
  • therapeutic angiogenesis

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Kanayasu-Toyoda, T., Ishii-Watabe, A., Kikuchi, Y., Kitagawa, H., Suzuki, H., Tamura, H., Tada, M., Suzuki, T., Mizuguchi, H., & Yamaguchi, T. (2018). Occludin as a functional marker of vascular endothelial cells on tube-forming activity. Journal of Cellular Physiology, 233(2), 1700-1711. https://doi.org/10.1002/jcp.26082