Occult injury in the residual lung after pneumonectomy in mice

Atsushi Tajima, Mitsutomo Kohno, Masazumi Watanabe, Yotaro Izumi, Sadatomo Tasaka, Ikuro Maruyama, Taku Miyasho, Koichi Kobayashi

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Abstract

Objectives: We aimed to determine the acute phase impact of pneumonectomy with respect to injury in the remaining lung using a murine model, and to investigate the profiles of inflammatory mediators including high mobility group box 1 protein (HMGB1) following surgery and administration of low dose intratracheal lipopolysaccharide. Methods: Mice received left pneumonectomy with intratracheal administration of either saline or lipopolysaccharide. Lung permeability index, lung wet-to-dry weight ratio, pathological findings, HMGB1 levels in bronchoalveolar lavage fluid (BALF) and plasma, and cytokine profiles in BALF were assessed 24 h after surgery. Results: Index of capillary permeability, lung water content, and neutrophil and macrophage counts in BALF were significantly increased by pneumonectomy. These parameters were highest in the mice with pneumonectomy with intratracheal administration of lipopolysaccharide. On lung pathology, neutrophil infiltration was prominent in the residual lung after pneumonectomy. HMGB1 levels were significantly higher in both BALF and plasma in the mice with pneumonectomy, and were highest in those with pneumonectomy and intratracheal administration of lipopolysaccharide. Pro-inflammatory cytokine levels including interferon-γ significantly increased in BALF in the mice with pneumonectomy. Conclusions: It was suggested that pneumonectomy itself may cause occult lung injury in the acute phase (24 h) of post-surgery which could be enhanced by inflammatory stimulus, such as bacterial component, leading to significant lung injury. HMGB1 might be involved in the pathogenesis of the occult lung injury.

Original languageEnglish
Pages (from-to)1114-1120
Number of pages7
JournalInteractive cardiovascular and thoracic surgery
Volume7
Issue number6
DOIs
Publication statusPublished - 2008 Dec 1

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Keywords

  • Endotoxin
  • HMGB1
  • Lung injury
  • Pneumonectomy

ASJC Scopus subject areas

  • Surgery
  • Pulmonary and Respiratory Medicine
  • Cardiology and Cardiovascular Medicine

Cite this

Tajima, A., Kohno, M., Watanabe, M., Izumi, Y., Tasaka, S., Maruyama, I., Miyasho, T., & Kobayashi, K. (2008). Occult injury in the residual lung after pneumonectomy in mice. Interactive cardiovascular and thoracic surgery, 7(6), 1114-1120. https://doi.org/10.1510/icvts.2007.170456