Occurrence of a novel cardiac natriuretic peptide in rats

Hiroshi Itoh, Kazuwa Nakao, Yoshikazu Kambayashi, Kiminori Hosoda, Yoshihiko Saito, Takayuki Yamada, Masashi Mukoyama, Hiroshi Arai, Gotaro Shirakami, Shin ichi Suga, Ikuyo Yoshida, Ken Inouye, Hiroo Imura

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)


We established a specific radioimmunoassay for the ring structure of "iso-ANP" and detected iso-ANP[23-46]-like immunoreactivity (-LI) in the rat atrium (2.76 ± 0.5 μg/g) and ventricle (13.9 ± 5.7 ng/g). High performance-gel permeation chromatography revealed that iso-ANP[23-46]-LI in the rat heart was composed of two components with molecular weights of 10K and 5K. In reverse phase-high performance liquid chromatography, the retention times of these components were clearly different from that of synthetic iso-ANP. The 5K peptide was demonstrated to be present in the perfusate from isolated rat hearts and possessed binding ability to ANP receptors. This natriuretic peptide was, however, not detectable in other tissues including the brain. We conclude that the novel cardiac natriuretic peptide distinct from iso-ANP and ANP occurs in the rat heart and is secreted from the heart.

Original languageEnglish
Pages (from-to)732-739
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - 1989 Jun 15
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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