Occurrence of a novel cardiac natriuretic peptide in rats

Hiroshi Itoh, Kazuwa Nakao, Yoshikazu Kambayashi, Kiminori Hosoda, Yoshihiko Saito, Takayuki Yamada, Masashi Mukoyama, Hiroshi Arai, Gotaro Shirakami, Shin ichi Suga, Ikuyo Yoshida, Ken Inouye, Hiroo Imura

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

We established a specific radioimmunoassay for the ring structure of "iso-ANP" and detected iso-ANP[23-46]-like immunoreactivity (-LI) in the rat atrium (2.76 ± 0.5 μg/g) and ventricle (13.9 ± 5.7 ng/g). High performance-gel permeation chromatography revealed that iso-ANP[23-46]-LI in the rat heart was composed of two components with molecular weights of 10K and 5K. In reverse phase-high performance liquid chromatography, the retention times of these components were clearly different from that of synthetic iso-ANP. The 5K peptide was demonstrated to be present in the perfusate from isolated rat hearts and possessed binding ability to ANP receptors. This natriuretic peptide was, however, not detectable in other tissues including the brain. We conclude that the novel cardiac natriuretic peptide distinct from iso-ANP and ANP occurs in the rat heart and is secreted from the heart.

Original languageEnglish
Pages (from-to)732-739
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume161
Issue number2
DOIs
Publication statusPublished - 1989 Jun 15
Externally publishedYes

Fingerprint

Natriuretic Peptides
Atrial Natriuretic Factor
Rats
Atrial Natriuretic Factor Receptors
Gel permeation chromatography
High performance liquid chromatography
Radioimmunoassay
Gel Chromatography
Brain
Molecular Weight
Molecular weight
High Pressure Liquid Chromatography
Tissue
Peptides

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Itoh, H., Nakao, K., Kambayashi, Y., Hosoda, K., Saito, Y., Yamada, T., ... Imura, H. (1989). Occurrence of a novel cardiac natriuretic peptide in rats. Biochemical and Biophysical Research Communications, 161(2), 732-739. https://doi.org/10.1016/0006-291X(89)92661-2

Occurrence of a novel cardiac natriuretic peptide in rats. / Itoh, Hiroshi; Nakao, Kazuwa; Kambayashi, Yoshikazu; Hosoda, Kiminori; Saito, Yoshihiko; Yamada, Takayuki; Mukoyama, Masashi; Arai, Hiroshi; Shirakami, Gotaro; Suga, Shin ichi; Yoshida, Ikuyo; Inouye, Ken; Imura, Hiroo.

In: Biochemical and Biophysical Research Communications, Vol. 161, No. 2, 15.06.1989, p. 732-739.

Research output: Contribution to journalArticle

Itoh, H, Nakao, K, Kambayashi, Y, Hosoda, K, Saito, Y, Yamada, T, Mukoyama, M, Arai, H, Shirakami, G, Suga, SI, Yoshida, I, Inouye, K & Imura, H 1989, 'Occurrence of a novel cardiac natriuretic peptide in rats', Biochemical and Biophysical Research Communications, vol. 161, no. 2, pp. 732-739. https://doi.org/10.1016/0006-291X(89)92661-2
Itoh, Hiroshi ; Nakao, Kazuwa ; Kambayashi, Yoshikazu ; Hosoda, Kiminori ; Saito, Yoshihiko ; Yamada, Takayuki ; Mukoyama, Masashi ; Arai, Hiroshi ; Shirakami, Gotaro ; Suga, Shin ichi ; Yoshida, Ikuyo ; Inouye, Ken ; Imura, Hiroo. / Occurrence of a novel cardiac natriuretic peptide in rats. In: Biochemical and Biophysical Research Communications. 1989 ; Vol. 161, No. 2. pp. 732-739.
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