Ocular-component-specific miRNA expression in a murine model of lens-induced myopia

Yasuhisa Tanaka, Toshihide Kurihara, Yumi Hagiwara, Shin Ichi Ikeda, Kiwako Mori, Xiaoyan Jiang, Hidemasa Torii, Kazuo Tsubota

Research output: Contribution to journalArticle

Abstract

To identify tissues and molecules involved in refractive myopic shift and axial length elongation in a murine lens-induced myopia model, we performed a comprehensive analysis of microRNA (miRNA) expression. Three weeks after negative 30 diopter lens fixation on three-week-old C57BL/6J mice, total RNA was extracted from individual ocular components including cornea, iris, lens, retina, retinal pigment epithelium (RPE)/choroid, and sclera tissue. The miRNA expression analysis was pooled from three samples and carried out using Agilent Mouse miRNA Microarray (8 × 60 K) miRBase21.0. The expression ratio was calculated, and differentially expressed miRNAs were extracted, using GeneSpring GX 14.5. Myopic induction showed a significant myopic refractive change, axial elongation, and choroidal thinning. Through the comprehensive miRNA analysis, several upregulated miRNAs (56 in cornea tissue, 13 in iris tissue, 6 in lens tissue, 0 in retina tissue, 29 in RPE/choroid tissue, and 30 in sclera tissue) and downregulated miRNAs (7 in cornea tissue, 28 in iris tissue, 17 in lens tissue, 9 in retina tissue, 7 in RPE/choroid tissue, and 40 in sclera tissue) were observed. Overlapping expression changes in miRNAs were also found in different ocular components. Some of this miRNA dysregulation may be functionally involved in refractive myopia shift and axial length elongation.

Original languageEnglish
Article number3629
JournalInternational journal of molecular sciences
Volume20
Issue number15
DOIs
Publication statusPublished - 2019 Aug 1

Fingerprint

myopia
Myopia
MicroRNAs
Lenses
lenses
Tissue
Sclera
Retinal Pigments
cornea
Choroid
epithelium
Retinal Pigment Epithelium
retina
Iris
pigments
Pigments
Cornea
elongation
Retina
Elongation

Keywords

  • Lens-induced myopia
  • miRNA
  • Ocular components

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Ocular-component-specific miRNA expression in a murine model of lens-induced myopia. / Tanaka, Yasuhisa; Kurihara, Toshihide; Hagiwara, Yumi; Ikeda, Shin Ichi; Mori, Kiwako; Jiang, Xiaoyan; Torii, Hidemasa; Tsubota, Kazuo.

In: International journal of molecular sciences, Vol. 20, No. 15, 3629, 01.08.2019.

Research output: Contribution to journalArticle

Tanaka, Yasuhisa ; Kurihara, Toshihide ; Hagiwara, Yumi ; Ikeda, Shin Ichi ; Mori, Kiwako ; Jiang, Xiaoyan ; Torii, Hidemasa ; Tsubota, Kazuo. / Ocular-component-specific miRNA expression in a murine model of lens-induced myopia. In: International journal of molecular sciences. 2019 ; Vol. 20, No. 15.
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