Odf2-deficient mother centrioles lack distal/subdistal appendages and the ability to generate primary cilia

Hiroaki Ishikawa, Akiharu Kubo, Shoichiro Tsukita, Sachiko Tsukita

Research output: Contribution to journalArticle

192 Citations (Scopus)

Abstract

Outer dense fibre 2 (Odf2; also known as cenexin) was initially identified as a main component of the sperm tail cytoskeleton, but was later shown to be a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Here we show that Odf2 expression is suppressed in mouse F9 cells when both alleles of Odf2 genes are deleted. Unexpectedly, the cell cycle of Odf2-/- cells does not seem to be affected. Immunofluorescence and ultrathin-section electron microscopy reveals that in Odf2-/- cells, distal/subdistal appendages disappear from mother centrioles, making it difficult to distinguish mother from daughter centrioles. In Odf2-/- cells, however, the formation of primary cilia is completely suppressed, although ∼25% of wild-type F9 cells are ciliated under the steady-state cell cycle. The loss of primary cilia in Odf2-/- F9 cells can be rescued by exogenous Odf2 expression. These findings indicate that Odf2 is indispensable for the formation of distal/subdistal appendages and the generation of primary cilia, but not for other cell-cycle-related centriolar functions.

Original languageEnglish
Pages (from-to)517-524
Number of pages8
JournalNature Cell Biology
Volume7
Issue number5
DOIs
Publication statusPublished - 2005 May
Externally publishedYes

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Centrioles
Cilia
Cell Cycle
Sperm Tail
Cytoskeleton
Fluorescent Antibody Technique
Electron Microscopy
Alleles
Genes
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Odf2-deficient mother centrioles lack distal/subdistal appendages and the ability to generate primary cilia. / Ishikawa, Hiroaki; Kubo, Akiharu; Tsukita, Shoichiro; Tsukita, Sachiko.

In: Nature Cell Biology, Vol. 7, No. 5, 05.2005, p. 517-524.

Research output: Contribution to journalArticle

Ishikawa, Hiroaki ; Kubo, Akiharu ; Tsukita, Shoichiro ; Tsukita, Sachiko. / Odf2-deficient mother centrioles lack distal/subdistal appendages and the ability to generate primary cilia. In: Nature Cell Biology. 2005 ; Vol. 7, No. 5. pp. 517-524.
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