Oil-Sealed Rgd-Modified Hydrogel Microwell Array with Size-Selective Permeation for Analysis on Exosomes from Single Cells

Chisaki Yamagata; Shun Itai; Yuta Kurashina; Makoto Asai; Ayuko Hoshino; Hiroaki Onoe

doi:10.1109/MEMS49605.2023.10052318

Oil-Sealed Rgd-Modified Hydrogel Microwell Array with Size-Selective Permeation for Analysis on Exosomes from Single Cells

Chisaki Yamagata, Shun Itai, Yuta Kurashina, Makoto Asai, Ayuko Hoshino, Hiroaki Onoe

Department of Mechanical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We propose an oil-sealed arginine-glycine-aspartate (RGD)-modified hydrogel microwell array for analyzing exosomes secreted from single cells. Our device realizes the collection of exosomes from single cells in parallel. The size-selective permeation of the RGD-modified alginate hydrogel allows both stable cell culturing of single or few cells in closed wells and the confinement of exosomes secreted in each space. The nutrients (< 20 nm) that are necessary for cell culture can pass through the hydrogel, while exosomes (30-150 nm) do not permeate the walls of microwells. The cell culture property and the permeability of our device were examined, showing the capability to collect exosomes from single cells. We believe that our device would contribute to understanding the mechanisms of various diseases.

Original language	English
Title of host publication	2023 IEEE 36th International Conference on Micro Electro Mechanical Systems, MEMS 2023
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	319-322
Number of pages	4
ISBN (Electronic)	9781665493086
DOIs	https://doi.org/10.1109/MEMS49605.2023.10052318
Publication status	Published - 2023
Event	36th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2023 - Munich, Germany Duration: 2023 Jan 15 → 2023 Jan 19

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume	2023-January
ISSN (Print)	1084-6999

Conference

Conference	36th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2023
Country/Territory	Germany
City	Munich
Period	23/1/15 → 23/1/19

Keywords

Alginate
Arginine-glycine-aspartate (RGD) motif
Exosome
Extracellular vesicle
Hydrogel
Microwell array
Single-cell analysis

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/MEMS49605.2023.10052318

Cite this

Yamagata, C., Itai, S., Kurashina, Y., Asai, M., Hoshino, A., & Onoe, H. (2023). Oil-Sealed Rgd-Modified Hydrogel Microwell Array with Size-Selective Permeation for Analysis on Exosomes from Single Cells. In 2023 IEEE 36th International Conference on Micro Electro Mechanical Systems, MEMS 2023 (pp. 319-322). (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2023-January). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMS49605.2023.10052318

Oil-Sealed Rgd-Modified Hydrogel Microwell Array with Size-Selective Permeation for Analysis on Exosomes from Single Cells. / Yamagata, Chisaki; Itai, Shun; Kurashina, Yuta et al.
2023 IEEE 36th International Conference on Micro Electro Mechanical Systems, MEMS 2023. Institute of Electrical and Electronics Engineers Inc., 2023. p. 319-322 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2023-January).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Yamagata, C, Itai, S, Kurashina, Y, Asai, M, Hoshino, A & Onoe, H 2023, Oil-Sealed Rgd-Modified Hydrogel Microwell Array with Size-Selective Permeation for Analysis on Exosomes from Single Cells. in 2023 IEEE 36th International Conference on Micro Electro Mechanical Systems, MEMS 2023. Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), vol. 2023-January, Institute of Electrical and Electronics Engineers Inc., pp. 319-322, 36th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2023, Munich, Germany, 23/1/15. https://doi.org/10.1109/MEMS49605.2023.10052318

Yamagata C, Itai S, Kurashina Y, Asai M, Hoshino A, Onoe H. Oil-Sealed Rgd-Modified Hydrogel Microwell Array with Size-Selective Permeation for Analysis on Exosomes from Single Cells. In 2023 IEEE 36th International Conference on Micro Electro Mechanical Systems, MEMS 2023. Institute of Electrical and Electronics Engineers Inc. 2023. p. 319-322. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMS49605.2023.10052318

Yamagata, Chisaki ; Itai, Shun ; Kurashina, Yuta et al. / Oil-Sealed Rgd-Modified Hydrogel Microwell Array with Size-Selective Permeation for Analysis on Exosomes from Single Cells. 2023 IEEE 36th International Conference on Micro Electro Mechanical Systems, MEMS 2023. Institute of Electrical and Electronics Engineers Inc., 2023. pp. 319-322 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

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abstract = "We propose an oil-sealed arginine-glycine-aspartate (RGD)-modified hydrogel microwell array for analyzing exosomes secreted from single cells. Our device realizes the collection of exosomes from single cells in parallel. The size-selective permeation of the RGD-modified alginate hydrogel allows both stable cell culturing of single or few cells in closed wells and the confinement of exosomes secreted in each space. The nutrients (< 20 nm) that are necessary for cell culture can pass through the hydrogel, while exosomes (30-150 nm) do not permeate the walls of microwells. The cell culture property and the permeability of our device were examined, showing the capability to collect exosomes from single cells. We believe that our device would contribute to understanding the mechanisms of various diseases.",

keywords = "Alginate, Arginine-glycine-aspartate (RGD) motif, Exosome, Extracellular vesicle, Hydrogel, Microwell array, Single-cell analysis",

author = "Chisaki Yamagata and Shun Itai and Yuta Kurashina and Makoto Asai and Ayuko Hoshino and Hiroaki Onoe",

note = "Funding Information: This work was partly supported by Research Project Keio 2040 (Creativity Initiative) at Keio University Global Research Institute, Japan. Publisher Copyright: {\textcopyright} 2023 IEEE.; 36th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2023 ; Conference date: 15-01-2023 Through 19-01-2023",

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AB - We propose an oil-sealed arginine-glycine-aspartate (RGD)-modified hydrogel microwell array for analyzing exosomes secreted from single cells. Our device realizes the collection of exosomes from single cells in parallel. The size-selective permeation of the RGD-modified alginate hydrogel allows both stable cell culturing of single or few cells in closed wells and the confinement of exosomes secreted in each space. The nutrients (< 20 nm) that are necessary for cell culture can pass through the hydrogel, while exosomes (30-150 nm) do not permeate the walls of microwells. The cell culture property and the permeability of our device were examined, showing the capability to collect exosomes from single cells. We believe that our device would contribute to understanding the mechanisms of various diseases.

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Oil-Sealed Rgd-Modified Hydrogel Microwell Array with Size-Selective Permeation for Analysis on Exosomes from Single Cells

Abstract

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Keywords

ASJC Scopus subject areas

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