Omentopexy enhances graft function in myocardial cell sheet transplantation

Ryo Suzuki, Fumiyuki Hattori, Yuji Itabashi, Masatoyo Yoshioka, Shinsuke Yuasa, Haruko Manabe-Kawaguchi, Mitsushige Murata, Shinji Makino, Kiyokazu Kokaji, Ryohei Yozu, Keiichi Fukuda

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Myocardial cell sheets (MCS) are a potentially valuable tool for tissue engineering aimed at heart regeneration. Several methods have recently been established for the fabrication of MCS. However, the lack of a sufficient blood supply has inhibited functional recovery of the MCS. To address this challenge, we combined MCS transplantation with omentopexy (OP), which utilizes omental tissue as a surgical flap. Rats were divided into five groups: sham, myocardial infarction (MI), MCS transplantation, OP, and MCS + OP. Histologic analysis revealed that MCS + OP drastically reversed MI-induced cardiac remodeling. Echocardiography revealed that MCS increased cardiac function, while OP had a synergistic beneficial effect with MCS transplantation. Immunofluorescence imaging showed that OP increased the survival of transplanted cardiomyocytes, and increased the blood supply through enhancement of angiogenesis and migration of small arteries into the MCS. Taken together, we concluded that OP is a promising strategy for the enhancement of graft function in MCS transplantation.

Original languageEnglish
Pages (from-to)353-359
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume387
Issue number2
DOIs
Publication statusPublished - 2009 Sep 18

Keywords

  • Infarct
  • Myocardial cell sheet
  • Omentopexy
  • Regenerative medicine
  • Transplant

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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