One-megabase sequence analysis of the human immunoglobulin λ gene locus

Kazuhiko Kawasaki; Shinsei Minoshima; Eriko Nakato; Kazunori Shibuya; Ai Shintani; James L. Schmeits; Jun Wang; Nobuyoshi Shimizu

doi:10.1101/gr.7.3.250

One-megabase sequence analysis of the human immunoglobulin λ gene locus

Kazuhiko Kawasaki, Shinsei Minoshima, Eriko Nakato, Kazunori Shibuya, Ai Shintani, James L. Schmeits, Jun Wang, Nobuyoshi Shimizu

Department of Molecular Biology

Research output: Contribution to journal › Article › peer-review

83 Citations (Scopus)

Abstract

A total of 1,025,415 bases of nucleotide sequence, including the entire human immunoglobulin λ gene locus has been determined. This is the largest contiguous human DNA sequence ever published. The sequence data revealed the organization of 36 potentially active V(λ) gene segments, 33 pseudogene segments, and several J(λ)-C(λ) gene segments. Among these 69 functional or nonfunctional V(λ) gene segments, 32 were newly discovered. These V(λ) gene segments are located within five gene-rich clusters and are divided into five clans based on sequence identity. Five potentially active nonimmunoglobulin genes were also detected within the λ gene locus, and two other genes were observed in the upstream region. Sequence organization suggests that large DNA duplications diversified the germ-line repertoire of the V(λ) gene segments.

Original language	English
Pages (from-to)	250-261
Number of pages	12
Journal	Genome Research
Volume	7
Issue number	3
DOIs	https://doi.org/10.1101/gr.7.3.250
Publication status	Published - 1997 Mar

ASJC Scopus subject areas

Genetics
Genetics(clinical)

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abstract = "A total of 1,025,415 bases of nucleotide sequence, including the entire human immunoglobulin λ gene locus has been determined. This is the largest contiguous human DNA sequence ever published. The sequence data revealed the organization of 36 potentially active V(λ) gene segments, 33 pseudogene segments, and several J(λ)-C(λ) gene segments. Among these 69 functional or nonfunctional V(λ) gene segments, 32 were newly discovered. These V(λ) gene segments are located within five gene-rich clusters and are divided into five clans based on sequence identity. Five potentially active nonimmunoglobulin genes were also detected within the λ gene locus, and two other genes were observed in the upstream region. Sequence organization suggests that large DNA duplications diversified the germ-line repertoire of the V(λ) gene segments.",

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AU - Kawasaki, Kazuhiko

AU - Minoshima, Shinsei

AU - Nakato, Eriko

AU - Shibuya, Kazunori

AU - Shintani, Ai

AU - Schmeits, James L.

AU - Wang, Jun

AU - Shimizu, Nobuyoshi

PY - 1997/3

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One-megabase sequence analysis of the human immunoglobulin λ gene locus

Abstract

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