One-step preparation of alkyl β-D-glucosaminide by the transglycosylation of chitosan and alcohol using purified exo-β-D-glucosaminidase

Shuichi Matsumura, Eiya Yao, Kazunobu Toshima

Research output: Contribution to journalArticle

19 Citations (Scopus)


Alkyl β-D-glucosaminides (RGlcN) were prepared by the novel direct transglycosylation reaction of chitosan and alcohol using the purified exo-β-D-glucosaminidase from the chitosan-assimilating strain, Penicillium funiculosum KY616. Using the exo-β-D-glucosaminidase, versatile synthetic intermediates, short and medium chain RGlcNs, were prepared in a maximum yield of higher than 480 mg/g-chitosan when chitosan and an alcohol were incubated at a temperature of 30°C for 2 d.

Original languageEnglish
Pages (from-to)451-456
Number of pages6
JournalBiotechnology Letters
Issue number5
Publication statusPublished - 1999 Jan 1



  • Alkyl β-D-glucosaminide
  • Chitosan
  • Enzymatic synthesis
  • Glycoside
  • exo-β-D-glucosaminidase

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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