Ontogeny and Multipotency of Neural Crest-Derived Stem Cells in Mouse Bone Marrow, Dorsal Root Ganglia, and Whisker Pad

Narihito Nagoshi, Shinsuke Shibata, Yoshiaki Kubota, Masaya Nakamura, Yasuo Nagai, Etsuko Satoh, Satoru Morikawa, Yohei Okada, Yo Mabuchi, Hiroyuki Katoh, Seiji Okada, Keiichi Fukuda, Toshio Suda, Yumi Matsuzaki, Yoshiaki Toyama, Hideyuki Okano

Research output: Contribution to journalArticle

242 Citations (Scopus)

Abstract

Although recent reports have described multipotent, self-renewing, neural crest-derived stem cells (NCSCs), the NCSCs in various adult rodent tissues have not been well characterized or compared. Here we identified NCSCs in the bone marrow (BM), dorsal root ganglia, and whisker pad and prospectively isolated them from adult transgenic mice encoding neural crest-specific P0-Cre/Floxed-EGFP and Wnt1-Cre/Floxed-EGFP. Cultured EGFP-positive cells formed neurosphere-like structures that expressed NCSC genes and could differentiate into neurons, glial cells, and myofibroblasts, but the frequency of the cell types was tissue source dependent. Interestingly, we observed NCSCs in the aorta-gonad-mesonephros region, circulating blood, and liver at the embryonic stage, suggesting that NCSCs migrate through the bloodstream to the BM and providing an explanation for how neural cells are generated from the BM. The identification of NCSCs in accessible adult tissue provides a new potential source for autologous cell therapy after nerve injury or disease.

Original languageEnglish
Pages (from-to)392-403
Number of pages12
JournalCell Stem Cell
Volume2
Issue number4
DOIs
Publication statusPublished - 2008 Apr 10

Fingerprint

Vibrissae
Neural Crest
Spinal Ganglia
Stem Cells
Bone Marrow
Mesonephros
Myofibroblasts
Neural Stem Cells
Gonads
Cell- and Tissue-Based Therapy
Neuroglia
Bone Marrow Cells
Transgenic Mice
Aorta
Rodentia
Neurons
Liver
Wounds and Injuries

Keywords

  • STEMCELL

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Medicine
  • Genetics

Cite this

Ontogeny and Multipotency of Neural Crest-Derived Stem Cells in Mouse Bone Marrow, Dorsal Root Ganglia, and Whisker Pad. / Nagoshi, Narihito; Shibata, Shinsuke; Kubota, Yoshiaki; Nakamura, Masaya; Nagai, Yasuo; Satoh, Etsuko; Morikawa, Satoru; Okada, Yohei; Mabuchi, Yo; Katoh, Hiroyuki; Okada, Seiji; Fukuda, Keiichi; Suda, Toshio; Matsuzaki, Yumi; Toyama, Yoshiaki; Okano, Hideyuki.

In: Cell Stem Cell, Vol. 2, No. 4, 10.04.2008, p. 392-403.

Research output: Contribution to journalArticle

Nagoshi, Narihito ; Shibata, Shinsuke ; Kubota, Yoshiaki ; Nakamura, Masaya ; Nagai, Yasuo ; Satoh, Etsuko ; Morikawa, Satoru ; Okada, Yohei ; Mabuchi, Yo ; Katoh, Hiroyuki ; Okada, Seiji ; Fukuda, Keiichi ; Suda, Toshio ; Matsuzaki, Yumi ; Toyama, Yoshiaki ; Okano, Hideyuki. / Ontogeny and Multipotency of Neural Crest-Derived Stem Cells in Mouse Bone Marrow, Dorsal Root Ganglia, and Whisker Pad. In: Cell Stem Cell. 2008 ; Vol. 2, No. 4. pp. 392-403.
@article{3ea8af61d9ef4cf7a5a657d7e08b8298,
title = "Ontogeny and Multipotency of Neural Crest-Derived Stem Cells in Mouse Bone Marrow, Dorsal Root Ganglia, and Whisker Pad",
abstract = "Although recent reports have described multipotent, self-renewing, neural crest-derived stem cells (NCSCs), the NCSCs in various adult rodent tissues have not been well characterized or compared. Here we identified NCSCs in the bone marrow (BM), dorsal root ganglia, and whisker pad and prospectively isolated them from adult transgenic mice encoding neural crest-specific P0-Cre/Floxed-EGFP and Wnt1-Cre/Floxed-EGFP. Cultured EGFP-positive cells formed neurosphere-like structures that expressed NCSC genes and could differentiate into neurons, glial cells, and myofibroblasts, but the frequency of the cell types was tissue source dependent. Interestingly, we observed NCSCs in the aorta-gonad-mesonephros region, circulating blood, and liver at the embryonic stage, suggesting that NCSCs migrate through the bloodstream to the BM and providing an explanation for how neural cells are generated from the BM. The identification of NCSCs in accessible adult tissue provides a new potential source for autologous cell therapy after nerve injury or disease.",
keywords = "STEMCELL",
author = "Narihito Nagoshi and Shinsuke Shibata and Yoshiaki Kubota and Masaya Nakamura and Yasuo Nagai and Etsuko Satoh and Satoru Morikawa and Yohei Okada and Yo Mabuchi and Hiroyuki Katoh and Seiji Okada and Keiichi Fukuda and Toshio Suda and Yumi Matsuzaki and Yoshiaki Toyama and Hideyuki Okano",
year = "2008",
month = "4",
day = "10",
doi = "10.1016/j.stem.2008.03.005",
language = "English",
volume = "2",
pages = "392--403",
journal = "Cell Stem Cell",
issn = "1934-5909",
publisher = "Cell Press",
number = "4",

}

TY - JOUR

T1 - Ontogeny and Multipotency of Neural Crest-Derived Stem Cells in Mouse Bone Marrow, Dorsal Root Ganglia, and Whisker Pad

AU - Nagoshi, Narihito

AU - Shibata, Shinsuke

AU - Kubota, Yoshiaki

AU - Nakamura, Masaya

AU - Nagai, Yasuo

AU - Satoh, Etsuko

AU - Morikawa, Satoru

AU - Okada, Yohei

AU - Mabuchi, Yo

AU - Katoh, Hiroyuki

AU - Okada, Seiji

AU - Fukuda, Keiichi

AU - Suda, Toshio

AU - Matsuzaki, Yumi

AU - Toyama, Yoshiaki

AU - Okano, Hideyuki

PY - 2008/4/10

Y1 - 2008/4/10

N2 - Although recent reports have described multipotent, self-renewing, neural crest-derived stem cells (NCSCs), the NCSCs in various adult rodent tissues have not been well characterized or compared. Here we identified NCSCs in the bone marrow (BM), dorsal root ganglia, and whisker pad and prospectively isolated them from adult transgenic mice encoding neural crest-specific P0-Cre/Floxed-EGFP and Wnt1-Cre/Floxed-EGFP. Cultured EGFP-positive cells formed neurosphere-like structures that expressed NCSC genes and could differentiate into neurons, glial cells, and myofibroblasts, but the frequency of the cell types was tissue source dependent. Interestingly, we observed NCSCs in the aorta-gonad-mesonephros region, circulating blood, and liver at the embryonic stage, suggesting that NCSCs migrate through the bloodstream to the BM and providing an explanation for how neural cells are generated from the BM. The identification of NCSCs in accessible adult tissue provides a new potential source for autologous cell therapy after nerve injury or disease.

AB - Although recent reports have described multipotent, self-renewing, neural crest-derived stem cells (NCSCs), the NCSCs in various adult rodent tissues have not been well characterized or compared. Here we identified NCSCs in the bone marrow (BM), dorsal root ganglia, and whisker pad and prospectively isolated them from adult transgenic mice encoding neural crest-specific P0-Cre/Floxed-EGFP and Wnt1-Cre/Floxed-EGFP. Cultured EGFP-positive cells formed neurosphere-like structures that expressed NCSC genes and could differentiate into neurons, glial cells, and myofibroblasts, but the frequency of the cell types was tissue source dependent. Interestingly, we observed NCSCs in the aorta-gonad-mesonephros region, circulating blood, and liver at the embryonic stage, suggesting that NCSCs migrate through the bloodstream to the BM and providing an explanation for how neural cells are generated from the BM. The identification of NCSCs in accessible adult tissue provides a new potential source for autologous cell therapy after nerve injury or disease.

KW - STEMCELL

UR - http://www.scopus.com/inward/record.url?scp=41449116604&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=41449116604&partnerID=8YFLogxK

U2 - 10.1016/j.stem.2008.03.005

DO - 10.1016/j.stem.2008.03.005

M3 - Article

VL - 2

SP - 392

EP - 403

JO - Cell Stem Cell

JF - Cell Stem Cell

SN - 1934-5909

IS - 4

ER -