Oocyte-specific linker histone in mammalian species

Mamoru Tanaka, Takahide Teranishi, Kei Miyakoshi, Wei Jiaxue, Kazunori Ueno, Tadashi Matsumoto, Yoshihisa Hattori, Yasunori Yoshimura

Research output: Contribution to journalArticle

Abstract

The linker histones constitute the major proteins bound to linker DNA, the DNA bridging nucleosome core particles. The composition of the linker histone fraction is tissue- and species-specific, as well as developmentally-regulated. As such, linker histones play a critical role in the higher order packaging of chromatin and thus, inevitably, in an impressive array of regulatory functions. Recently, we uncovered a mammalian oocyte-specific linker histone H1oo homologous to the cs-H1 of sea urchin and to the B4 of the frog in the course of a differential screening project. H1oo protein localized to the intact germinal vesicle (GV) of preovulatory oocytes, to the condensed chromosomes of ovulated oocytes arrested at metaphase (M) II, and to the first polar body. The pronuclei were both positive at the 1-cell stage. Nuclear staining, however, was reduced in 2-cell embryos and was no longer detectable at the 4-cell stage of embryonic development. These findings indicate oocyte-specific linker histones may play some roles in genome-wide reprogramming in vertebrate eggs.

Original languageEnglish
Pages (from-to)61-65
Number of pages5
JournalJournal of Mammalian Ova Research
Volume19
Issue number3
DOIs
Publication statusPublished - 2002

Fingerprint

Histones
Oocytes
Polar Bodies
Sea Urchins
Nucleosomes
DNA
Product Packaging
Metaphase
Anura
Eggs
Chromatin
Embryonic Development
Vertebrates
Proteins
Embryonic Structures
Chromosomes
Genome
Staining and Labeling

Keywords

  • Mouse
  • Oocyte-specific linker histone
  • Reprogramming

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

Cite this

Tanaka, M., Teranishi, T., Miyakoshi, K., Jiaxue, W., Ueno, K., Matsumoto, T., ... Yoshimura, Y. (2002). Oocyte-specific linker histone in mammalian species. Journal of Mammalian Ova Research, 19(3), 61-65. https://doi.org/10.1274/jmor.19.61

Oocyte-specific linker histone in mammalian species. / Tanaka, Mamoru; Teranishi, Takahide; Miyakoshi, Kei; Jiaxue, Wei; Ueno, Kazunori; Matsumoto, Tadashi; Hattori, Yoshihisa; Yoshimura, Yasunori.

In: Journal of Mammalian Ova Research, Vol. 19, No. 3, 2002, p. 61-65.

Research output: Contribution to journalArticle

Tanaka, M, Teranishi, T, Miyakoshi, K, Jiaxue, W, Ueno, K, Matsumoto, T, Hattori, Y & Yoshimura, Y 2002, 'Oocyte-specific linker histone in mammalian species', Journal of Mammalian Ova Research, vol. 19, no. 3, pp. 61-65. https://doi.org/10.1274/jmor.19.61
Tanaka, Mamoru ; Teranishi, Takahide ; Miyakoshi, Kei ; Jiaxue, Wei ; Ueno, Kazunori ; Matsumoto, Tadashi ; Hattori, Yoshihisa ; Yoshimura, Yasunori. / Oocyte-specific linker histone in mammalian species. In: Journal of Mammalian Ova Research. 2002 ; Vol. 19, No. 3. pp. 61-65.
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