Optical bioimaging

From living tissue to a single molecule: Imaging and functional analysis of blood flow in organic microdilution

Haruyuki Minamitani, Kosuke Tsukada, Eiichi Sekizuka, Chikara Oshio

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Activity of blood cells, erythrocytes, leucocytes, and platelets, in microcirculation was observed by using an intravital microscope and confocal laser scanning microscope connected with an image processing system including fluorescence and phosphorescence emission methods. Dynamic functions of the blood flow were mainly observed in mesentery, brain, and liver tissues of rats. The results are summarized as follows: Deformability of diabetic erythrocytes was significantly lower than that of healthy controls, particularly at high shear rate. The spring constant and Young's modulus of diabetic erythrocytes obviously stiffened, making them hard to deform in the capillary. During hemorrhagic shock and thrombosis, flow velocity and oxygen partial pressure of blood decreased in the brain and liver tissues that can be visualized by using FITC stained erythrocytes and Pd-porphyrin derivative as a pO2 probe. Platelet adhesion and thrombus formation in the micro-vessels accelerated under the photodynamic reaction; diabetic platelets showed augmented adhesion and aggregation on the vessel wall which was followed by acute thromboembolism. Active oxygen radicals take part in thrombus formation, accompanied with adhesion of the activated leucocytes. Fluorescent dye probes, rhodamine G and acridine orange, are quite useful for visualization of the flow behavior of platelets and leucocytes, respectively.

Original languageEnglish
Pages (from-to)227-233
Number of pages7
JournalJournal of Pharmacological Sciences
Volume93
Issue number3
DOIs
Publication statusPublished - 2003 Nov
Externally publishedYes

Fingerprint

Blood Platelets
Thrombosis
Leukocytes
Erythrocytes
Fluorescent Dyes
Reactive Oxygen Species
Erythrocyte Deformability
Acridine Orange
Rhodamines
Mesentery
Hemorrhagic Shock
Fluorescein-5-isothiocyanate
Partial Pressure
Elastic Modulus
Liver
Thromboembolism
Brain
Microcirculation
Blood Cells
Lasers

Keywords

  • Active oxygen
  • Erythrocyte deformability
  • Hemostasis
  • Image analysis
  • Microcirculation

ASJC Scopus subject areas

  • Pharmacology

Cite this

Optical bioimaging : From living tissue to a single molecule: Imaging and functional analysis of blood flow in organic microdilution. / Minamitani, Haruyuki; Tsukada, Kosuke; Sekizuka, Eiichi; Oshio, Chikara.

In: Journal of Pharmacological Sciences, Vol. 93, No. 3, 11.2003, p. 227-233.

Research output: Contribution to journalArticle

@article{e9d4adfca61041f5817a2150240e6461,
title = "Optical bioimaging: From living tissue to a single molecule: Imaging and functional analysis of blood flow in organic microdilution",
abstract = "Activity of blood cells, erythrocytes, leucocytes, and platelets, in microcirculation was observed by using an intravital microscope and confocal laser scanning microscope connected with an image processing system including fluorescence and phosphorescence emission methods. Dynamic functions of the blood flow were mainly observed in mesentery, brain, and liver tissues of rats. The results are summarized as follows: Deformability of diabetic erythrocytes was significantly lower than that of healthy controls, particularly at high shear rate. The spring constant and Young's modulus of diabetic erythrocytes obviously stiffened, making them hard to deform in the capillary. During hemorrhagic shock and thrombosis, flow velocity and oxygen partial pressure of blood decreased in the brain and liver tissues that can be visualized by using FITC stained erythrocytes and Pd-porphyrin derivative as a pO2 probe. Platelet adhesion and thrombus formation in the micro-vessels accelerated under the photodynamic reaction; diabetic platelets showed augmented adhesion and aggregation on the vessel wall which was followed by acute thromboembolism. Active oxygen radicals take part in thrombus formation, accompanied with adhesion of the activated leucocytes. Fluorescent dye probes, rhodamine G and acridine orange, are quite useful for visualization of the flow behavior of platelets and leucocytes, respectively.",
keywords = "Active oxygen, Erythrocyte deformability, Hemostasis, Image analysis, Microcirculation",
author = "Haruyuki Minamitani and Kosuke Tsukada and Eiichi Sekizuka and Chikara Oshio",
year = "2003",
month = "11",
doi = "10.1254/jphs.93.227",
language = "English",
volume = "93",
pages = "227--233",
journal = "Journal of Pharmacological Sciences",
issn = "1347-8613",
publisher = "Japanese Pharmacological Society",
number = "3",

}

TY - JOUR

T1 - Optical bioimaging

T2 - From living tissue to a single molecule: Imaging and functional analysis of blood flow in organic microdilution

AU - Minamitani, Haruyuki

AU - Tsukada, Kosuke

AU - Sekizuka, Eiichi

AU - Oshio, Chikara

PY - 2003/11

Y1 - 2003/11

N2 - Activity of blood cells, erythrocytes, leucocytes, and platelets, in microcirculation was observed by using an intravital microscope and confocal laser scanning microscope connected with an image processing system including fluorescence and phosphorescence emission methods. Dynamic functions of the blood flow were mainly observed in mesentery, brain, and liver tissues of rats. The results are summarized as follows: Deformability of diabetic erythrocytes was significantly lower than that of healthy controls, particularly at high shear rate. The spring constant and Young's modulus of diabetic erythrocytes obviously stiffened, making them hard to deform in the capillary. During hemorrhagic shock and thrombosis, flow velocity and oxygen partial pressure of blood decreased in the brain and liver tissues that can be visualized by using FITC stained erythrocytes and Pd-porphyrin derivative as a pO2 probe. Platelet adhesion and thrombus formation in the micro-vessels accelerated under the photodynamic reaction; diabetic platelets showed augmented adhesion and aggregation on the vessel wall which was followed by acute thromboembolism. Active oxygen radicals take part in thrombus formation, accompanied with adhesion of the activated leucocytes. Fluorescent dye probes, rhodamine G and acridine orange, are quite useful for visualization of the flow behavior of platelets and leucocytes, respectively.

AB - Activity of blood cells, erythrocytes, leucocytes, and platelets, in microcirculation was observed by using an intravital microscope and confocal laser scanning microscope connected with an image processing system including fluorescence and phosphorescence emission methods. Dynamic functions of the blood flow were mainly observed in mesentery, brain, and liver tissues of rats. The results are summarized as follows: Deformability of diabetic erythrocytes was significantly lower than that of healthy controls, particularly at high shear rate. The spring constant and Young's modulus of diabetic erythrocytes obviously stiffened, making them hard to deform in the capillary. During hemorrhagic shock and thrombosis, flow velocity and oxygen partial pressure of blood decreased in the brain and liver tissues that can be visualized by using FITC stained erythrocytes and Pd-porphyrin derivative as a pO2 probe. Platelet adhesion and thrombus formation in the micro-vessels accelerated under the photodynamic reaction; diabetic platelets showed augmented adhesion and aggregation on the vessel wall which was followed by acute thromboembolism. Active oxygen radicals take part in thrombus formation, accompanied with adhesion of the activated leucocytes. Fluorescent dye probes, rhodamine G and acridine orange, are quite useful for visualization of the flow behavior of platelets and leucocytes, respectively.

KW - Active oxygen

KW - Erythrocyte deformability

KW - Hemostasis

KW - Image analysis

KW - Microcirculation

UR - http://www.scopus.com/inward/record.url?scp=0347506016&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0347506016&partnerID=8YFLogxK

U2 - 10.1254/jphs.93.227

DO - 10.1254/jphs.93.227

M3 - Article

VL - 93

SP - 227

EP - 233

JO - Journal of Pharmacological Sciences

JF - Journal of Pharmacological Sciences

SN - 1347-8613

IS - 3

ER -