Optical coefficient measurements using bulk living tissue by an optical fiber puncture with FOV change

Haruna Nakazawa, Marika Doi, Emiyu Ogawa, Tsunenori Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

To avoid an instability of the optical coefficient measurement using sliced tissue preparation, we proposed the combination of light intensity measurement through an optical fiber puncturing into a bulk tissue varying field of view (FOV) and ray tracing calculation using Monte-Carlo method. The optical coefficients of myocardium such as absorption coefficient μa, scattering coefficient μs, and anisotropic parameter g are used in the myocardium optical propagation. Since optical coefficients obtained using thin sliced tissue could be instable because they are affected by dehydration and intracellular fluid effusion on the sample surface, variety of coefficients have been reported over individual optical differences of living samples. The proposed method which combined the experiment using the bulk tissue with ray tracing calculation were performed. In this method, a 200 μmΦ high-NA silica fiber installed in a 21G needle was punctured up to the bottom of the myocardial bulk tissue over 3 cm in thickness to measure light intensity changing the fiber-tip depth and FOV. We found that the measured attenuation coefficients decreased as the FOV increased. The ray trace calculation represented the same FOV dependence in above mentioned experimental result. We think our particular fiber punctured measurement using bulk tissue varying FOV with Inverse Monte-Carlo method might be useful to obtain the optical coefficients to avoid sample preparation instabilities.

Original languageEnglish
Title of host publicationOptical Interactions with Tissue and Cells XXIX
PublisherSPIE
Volume10492
ISBN (Electronic)9781510614697
DOIs
Publication statusPublished - 2018 Jan 1
EventOptical Interactions with Tissue and Cells XXIX 2018 - San Francisco, United States
Duration: 2018 Jan 292018 Jan 30

Other

OtherOptical Interactions with Tissue and Cells XXIX 2018
CountryUnited States
CitySan Francisco
Period18/1/2918/1/30

Keywords

  • bulk tissue
  • field of view
  • light intensity measurement
  • Optical coefficients
  • ray tracing calculation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Nakazawa, H., Doi, M., Ogawa, E., & Arai, T. (2018). Optical coefficient measurements using bulk living tissue by an optical fiber puncture with FOV change. In Optical Interactions with Tissue and Cells XXIX (Vol. 10492). [104920P] SPIE. https://doi.org/10.1117/12.2289332