Optical observation of DNA motion during and immediately after nanopore translocation

Hirohito Yamazaki, Shintaro Ito, Keiko Esashika, Toshiharu Saiki

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    6 Citations (Scopus)

    Abstract

    Herein, we report an optical nanopore detection system capable of investigating DNA motion near nanopores not only during translocation but also post-translocation with submillisecond resolution. Using our optical nanopore detection system, we observed the voltage dependence of the dwell time of both 10-kbp double-stranded DNA (dsDNA) and lambda DNA in the excitation volume, which can be attributed to the drift-dominated motion. We found that the lambda DNA had slower drift motion than 10-kbp dsDNA, indicating that DNA with longer gyration experiences a lower nonuniform electric force.

    Original languageEnglish
    Article number017001
    JournalApplied Physics Express
    Volume9
    Issue number1
    DOIs
    Publication statusPublished - 2016 Jan

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    ASJC Scopus subject areas

    • Engineering(all)
    • Physics and Astronomy(all)

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