TY - JOUR
T1 - Optical observation of DNA motion during and immediately after nanopore translocation
AU - Yamazaki, Hirohito
AU - Ito, Shintaro
AU - Esashika, Keiko
AU - Saiki, Toshiharu
N1 - Publisher Copyright:
© 2016 The Japan Society of Applied Physics.
PY - 2016/1
Y1 - 2016/1
N2 - Herein, we report an optical nanopore detection system capable of investigating DNA motion near nanopores not only during translocation but also post-translocation with submillisecond resolution. Using our optical nanopore detection system, we observed the voltage dependence of the dwell time of both 10-kbp double-stranded DNA (dsDNA) and lambda DNA in the excitation volume, which can be attributed to the drift-dominated motion. We found that the lambda DNA had slower drift motion than 10-kbp dsDNA, indicating that DNA with longer gyration experiences a lower nonuniform electric force.
AB - Herein, we report an optical nanopore detection system capable of investigating DNA motion near nanopores not only during translocation but also post-translocation with submillisecond resolution. Using our optical nanopore detection system, we observed the voltage dependence of the dwell time of both 10-kbp double-stranded DNA (dsDNA) and lambda DNA in the excitation volume, which can be attributed to the drift-dominated motion. We found that the lambda DNA had slower drift motion than 10-kbp dsDNA, indicating that DNA with longer gyration experiences a lower nonuniform electric force.
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U2 - 10.7567/APEX.9.017001
DO - 10.7567/APEX.9.017001
M3 - Article
AN - SCOPUS:84952332644
SN - 1882-0778
VL - 9
JO - Applied Physics Express
JF - Applied Physics Express
IS - 1
M1 - 017001
ER -