TY - JOUR
T1 - Optical observation of DNA translocation through Al2O3 sputtered silicon nanopores in porous membrane
AU - Yamazaki, Hirohito
AU - Ito, Shintaro
AU - Esashika, Keiko
AU - Taguchi, Yoshihiro
AU - Saiki, Toshiharu
N1 - Funding Information:
The authors thank Yoshiaki Takaba at Taguchi Laboratory, Keio University, for assisting in the numerical simulation of static electric field. This research was supported by a Grant-in-Aid for Scientific Research(S) and JSPS Fellows, Grant Nos. 24226006 and 15J04005, from the Ministry of Education, Culture, Sport, Science.
Publisher Copyright:
© 2016, Springer-Verlag Berlin Heidelberg.
PY - 2016/3/1
Y1 - 2016/3/1
N2 - Nanopore sensors are being developed as a platform for analyzing single DNA, RNA, and protein. In nanopore sensors, ionic current measurement is widely used and proof-of-concept of nanopore DNA sequencing by it has been demonstrated by previous studies. Recently, we proposed an alternative platform of nanopore DNA sequencing that incorporates ultraviolet light and porous silicon membrane to perform high-throughput measurement. In the development of our DNA sequencing platform, controlling nanopore size in porous silicon membrane is essential but remains a challenge. Here, we report on observation of DNA translocation through Al2O3 sputtered silicon nanopores (Al2O3 nanopores) by our optical scheme. Electromagnetic wave simulation was performed to analyze the excitation volume on Al2O3 nanopores generated by focused ultraviolet light. In the experiment, DNA translocation time through Al2O3 nanopores was compared with that of silicon nanopores and we examined the effect of nanopore density and thickness of membrane by supplementing the static electric field simulation.
AB - Nanopore sensors are being developed as a platform for analyzing single DNA, RNA, and protein. In nanopore sensors, ionic current measurement is widely used and proof-of-concept of nanopore DNA sequencing by it has been demonstrated by previous studies. Recently, we proposed an alternative platform of nanopore DNA sequencing that incorporates ultraviolet light and porous silicon membrane to perform high-throughput measurement. In the development of our DNA sequencing platform, controlling nanopore size in porous silicon membrane is essential but remains a challenge. Here, we report on observation of DNA translocation through Al2O3 sputtered silicon nanopores (Al2O3 nanopores) by our optical scheme. Electromagnetic wave simulation was performed to analyze the excitation volume on Al2O3 nanopores generated by focused ultraviolet light. In the experiment, DNA translocation time through Al2O3 nanopores was compared with that of silicon nanopores and we examined the effect of nanopore density and thickness of membrane by supplementing the static electric field simulation.
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U2 - 10.1007/s00339-016-9764-9
DO - 10.1007/s00339-016-9764-9
M3 - Article
AN - SCOPUS:84959327342
SN - 0947-8396
VL - 122
SP - 1
EP - 6
JO - Applied Physics A: Materials Science and Processing
JF - Applied Physics A: Materials Science and Processing
IS - 3
M1 - 216
ER -