Optimal conditions for cardiac catheter ablation using photodynamic therapy

Takehiro Kimura, Seiji Takatsuki, Shunichiro Miyoshi, Mei Takahashi, Emiyu Ogawa, Yoshinori Katsumata, Takahiko Nishiyama, Nobuhiro Nishiyama, Yoko Tanimoto, Yoshiyasu Aizawa, Tsunenori Arai, Keiichi Fukuda

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Aims Photodynamic therapy (PDT) is based on non-thermal injury mediated by singlet oxygen species and is used clinically in cancer therapy. In our continuing efforts to apply this technology to cardiac catheter ablation, we clarified the optimal condition for creating PDT-mediated lesions using a laser catheter. Methods and results In a total of 35 canines, we applied a laser directly to the epicardium of the beating heart during open-chest surgery at 15 min after administration of a photosensitizer, talaporfin sodium. We evaluated the lesion size (depth and width) using hematoxylin-eosin staining under varying conditions as follows: laser output (5, 10, 20 W/cm<sup>2</sup>), irradiation time (0-60 s), photosensitizer concentration (0, 2.5, 5 mg/kg), blood oxygen concentration (103.5 ± 2.1 vs. 548.0 ± 18.4 torr), and contact force applied during irradiations (low: <20 g, high: >20 g). A laser irradiation at 20 W/cm<sup>2</sup> for 60 s under 5 mg/kg (29 μg/mL) of photosensitizer induced a lesion 8.7 ± 0.8 mm deep and 5.2 ± 0.2 mm wide. The lesion size was thus positively correlated to the laser power, irradiation time, and photosensitizer concentration, and was independent of the applied contact force and oxygen concentration. In addition, the concentration of the photosensitizer strongly correlated with the changes in the pulse oximetry data and fluorescence of the backscattering laser, suggesting that a clinically appropriate condition could be estimated in real time. Conclusion Photodynamic therapy-mediated cardiac lesions might be controllable by regulating the photosensitizer concentration, laser output, and irradiation time.

Original languageEnglish
Pages (from-to)1309-1315
Number of pages7
JournalEuropace
Volume17
Issue number8
DOIs
Publication statusPublished - 2015 Jun 30

Fingerprint

Cardiac Catheters
Catheter Ablation
Photochemotherapy
Photosensitizing Agents
Lasers
Oxygen
Singlet Oxygen
Oximetry
Pericardium
Hematoxylin
Eosine Yellowish-(YS)
Canidae
Thorax
Catheters
Fluorescence
Staining and Labeling
Technology
Wounds and Injuries

Keywords

  • Ablation
  • Arrhythmia
  • Photodynamic therapy
  • Talaporfin sodium

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Optimal conditions for cardiac catheter ablation using photodynamic therapy. / Kimura, Takehiro; Takatsuki, Seiji; Miyoshi, Shunichiro; Takahashi, Mei; Ogawa, Emiyu; Katsumata, Yoshinori; Nishiyama, Takahiko; Nishiyama, Nobuhiro; Tanimoto, Yoko; Aizawa, Yoshiyasu; Arai, Tsunenori; Fukuda, Keiichi.

In: Europace, Vol. 17, No. 8, 30.06.2015, p. 1309-1315.

Research output: Contribution to journalArticle

Kimura, T, Takatsuki, S, Miyoshi, S, Takahashi, M, Ogawa, E, Katsumata, Y, Nishiyama, T, Nishiyama, N, Tanimoto, Y, Aizawa, Y, Arai, T & Fukuda, K 2015, 'Optimal conditions for cardiac catheter ablation using photodynamic therapy', Europace, vol. 17, no. 8, pp. 1309-1315. https://doi.org/10.1093/europace/euu335
Kimura T, Takatsuki S, Miyoshi S, Takahashi M, Ogawa E, Katsumata Y et al. Optimal conditions for cardiac catheter ablation using photodynamic therapy. Europace. 2015 Jun 30;17(8):1309-1315. https://doi.org/10.1093/europace/euu335
Kimura, Takehiro ; Takatsuki, Seiji ; Miyoshi, Shunichiro ; Takahashi, Mei ; Ogawa, Emiyu ; Katsumata, Yoshinori ; Nishiyama, Takahiko ; Nishiyama, Nobuhiro ; Tanimoto, Yoko ; Aizawa, Yoshiyasu ; Arai, Tsunenori ; Fukuda, Keiichi. / Optimal conditions for cardiac catheter ablation using photodynamic therapy. In: Europace. 2015 ; Vol. 17, No. 8. pp. 1309-1315.
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AU - Miyoshi, Shunichiro

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AU - Ogawa, Emiyu

AU - Katsumata, Yoshinori

AU - Nishiyama, Takahiko

AU - Nishiyama, Nobuhiro

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N2 - Aims Photodynamic therapy (PDT) is based on non-thermal injury mediated by singlet oxygen species and is used clinically in cancer therapy. In our continuing efforts to apply this technology to cardiac catheter ablation, we clarified the optimal condition for creating PDT-mediated lesions using a laser catheter. Methods and results In a total of 35 canines, we applied a laser directly to the epicardium of the beating heart during open-chest surgery at 15 min after administration of a photosensitizer, talaporfin sodium. We evaluated the lesion size (depth and width) using hematoxylin-eosin staining under varying conditions as follows: laser output (5, 10, 20 W/cm2), irradiation time (0-60 s), photosensitizer concentration (0, 2.5, 5 mg/kg), blood oxygen concentration (103.5 ± 2.1 vs. 548.0 ± 18.4 torr), and contact force applied during irradiations (low: <20 g, high: >20 g). A laser irradiation at 20 W/cm2 for 60 s under 5 mg/kg (29 μg/mL) of photosensitizer induced a lesion 8.7 ± 0.8 mm deep and 5.2 ± 0.2 mm wide. The lesion size was thus positively correlated to the laser power, irradiation time, and photosensitizer concentration, and was independent of the applied contact force and oxygen concentration. In addition, the concentration of the photosensitizer strongly correlated with the changes in the pulse oximetry data and fluorescence of the backscattering laser, suggesting that a clinically appropriate condition could be estimated in real time. Conclusion Photodynamic therapy-mediated cardiac lesions might be controllable by regulating the photosensitizer concentration, laser output, and irradiation time.

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KW - Ablation

KW - Arrhythmia

KW - Photodynamic therapy

KW - Talaporfin sodium

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