Abstract
Aims Photodynamic therapy (PDT) is based on non-thermal injury mediated by singlet oxygen species and is used clinically in cancer therapy. In our continuing efforts to apply this technology to cardiac catheter ablation, we clarified the optimal condition for creating PDT-mediated lesions using a laser catheter. Methods and results In a total of 35 canines, we applied a laser directly to the epicardium of the beating heart during open-chest surgery at 15 min after administration of a photosensitizer, talaporfin sodium. We evaluated the lesion size (depth and width) using hematoxylin-eosin staining under varying conditions as follows: laser output (5, 10, 20 W/cm<sup>2</sup>), irradiation time (0-60 s), photosensitizer concentration (0, 2.5, 5 mg/kg), blood oxygen concentration (103.5 ± 2.1 vs. 548.0 ± 18.4 torr), and contact force applied during irradiations (low: <20 g, high: >20 g). A laser irradiation at 20 W/cm<sup>2</sup> for 60 s under 5 mg/kg (29 μg/mL) of photosensitizer induced a lesion 8.7 ± 0.8 mm deep and 5.2 ± 0.2 mm wide. The lesion size was thus positively correlated to the laser power, irradiation time, and photosensitizer concentration, and was independent of the applied contact force and oxygen concentration. In addition, the concentration of the photosensitizer strongly correlated with the changes in the pulse oximetry data and fluorescence of the backscattering laser, suggesting that a clinically appropriate condition could be estimated in real time. Conclusion Photodynamic therapy-mediated cardiac lesions might be controllable by regulating the photosensitizer concentration, laser output, and irradiation time.
| Original language | English |
|---|---|
| Pages (from-to) | 1309-1315 |
| Number of pages | 7 |
| Journal | Europace |
| Volume | 17 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2015 Jun 30 |
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Keywords
- Ablation
- Arrhythmia
- Photodynamic therapy
- Talaporfin sodium
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
- Physiology (medical)
Cite this
Optimal conditions for cardiac catheter ablation using photodynamic therapy. / Kimura, Takehiro; Takatsuki, Seiji; Miyoshi, Shunichiro; Takahashi, Mei; Ogawa, Emiyu; Katsumata, Yoshinori; Nishiyama, Takahiko; Nishiyama, Nobuhiro; Tanimoto, Yoko; Aizawa, Yoshiyasu; Arai, Tsunenori; Fukuda, Keiichi.
In: Europace, Vol. 17, No. 8, 30.06.2015, p. 1309-1315.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Optimal conditions for cardiac catheter ablation using photodynamic therapy
AU - Kimura, Takehiro
AU - Takatsuki, Seiji
AU - Miyoshi, Shunichiro
AU - Takahashi, Mei
AU - Ogawa, Emiyu
AU - Katsumata, Yoshinori
AU - Nishiyama, Takahiko
AU - Nishiyama, Nobuhiro
AU - Tanimoto, Yoko
AU - Aizawa, Yoshiyasu
AU - Arai, Tsunenori
AU - Fukuda, Keiichi
PY - 2015/6/30
Y1 - 2015/6/30
N2 - Aims Photodynamic therapy (PDT) is based on non-thermal injury mediated by singlet oxygen species and is used clinically in cancer therapy. In our continuing efforts to apply this technology to cardiac catheter ablation, we clarified the optimal condition for creating PDT-mediated lesions using a laser catheter. Methods and results In a total of 35 canines, we applied a laser directly to the epicardium of the beating heart during open-chest surgery at 15 min after administration of a photosensitizer, talaporfin sodium. We evaluated the lesion size (depth and width) using hematoxylin-eosin staining under varying conditions as follows: laser output (5, 10, 20 W/cm2), irradiation time (0-60 s), photosensitizer concentration (0, 2.5, 5 mg/kg), blood oxygen concentration (103.5 ± 2.1 vs. 548.0 ± 18.4 torr), and contact force applied during irradiations (low: <20 g, high: >20 g). A laser irradiation at 20 W/cm2 for 60 s under 5 mg/kg (29 μg/mL) of photosensitizer induced a lesion 8.7 ± 0.8 mm deep and 5.2 ± 0.2 mm wide. The lesion size was thus positively correlated to the laser power, irradiation time, and photosensitizer concentration, and was independent of the applied contact force and oxygen concentration. In addition, the concentration of the photosensitizer strongly correlated with the changes in the pulse oximetry data and fluorescence of the backscattering laser, suggesting that a clinically appropriate condition could be estimated in real time. Conclusion Photodynamic therapy-mediated cardiac lesions might be controllable by regulating the photosensitizer concentration, laser output, and irradiation time.
AB - Aims Photodynamic therapy (PDT) is based on non-thermal injury mediated by singlet oxygen species and is used clinically in cancer therapy. In our continuing efforts to apply this technology to cardiac catheter ablation, we clarified the optimal condition for creating PDT-mediated lesions using a laser catheter. Methods and results In a total of 35 canines, we applied a laser directly to the epicardium of the beating heart during open-chest surgery at 15 min after administration of a photosensitizer, talaporfin sodium. We evaluated the lesion size (depth and width) using hematoxylin-eosin staining under varying conditions as follows: laser output (5, 10, 20 W/cm2), irradiation time (0-60 s), photosensitizer concentration (0, 2.5, 5 mg/kg), blood oxygen concentration (103.5 ± 2.1 vs. 548.0 ± 18.4 torr), and contact force applied during irradiations (low: <20 g, high: >20 g). A laser irradiation at 20 W/cm2 for 60 s under 5 mg/kg (29 μg/mL) of photosensitizer induced a lesion 8.7 ± 0.8 mm deep and 5.2 ± 0.2 mm wide. The lesion size was thus positively correlated to the laser power, irradiation time, and photosensitizer concentration, and was independent of the applied contact force and oxygen concentration. In addition, the concentration of the photosensitizer strongly correlated with the changes in the pulse oximetry data and fluorescence of the backscattering laser, suggesting that a clinically appropriate condition could be estimated in real time. Conclusion Photodynamic therapy-mediated cardiac lesions might be controllable by regulating the photosensitizer concentration, laser output, and irradiation time.
KW - Ablation
KW - Arrhythmia
KW - Photodynamic therapy
KW - Talaporfin sodium
UR - http://www.scopus.com/inward/record.url?scp=84939609281&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84939609281&partnerID=8YFLogxK
U2 - 10.1093/europace/euu335
DO - 10.1093/europace/euu335
M3 - Article
C2 - 25564546
AN - SCOPUS:84939609281
VL - 17
SP - 1309
EP - 1315
JO - Europace
JF - Europace
SN - 1099-5129
IS - 8
ER -