Abstract
Homogeneous DNA assays using gold nanoparticles (AuNPs) require the reduction of nonspecific binding between AuNPs to improve sensitivity in detecting the target molecule. In this study, we employed alkanethiol self-assembled monolayers (SAMs) for modifying the AuNP surface to attain both good dispersability and high hybridization efficiency. The alkanethiol SAMs enhance the repulsive interaction between AuNPs, reducing nonspecific binding and promoting the extension of surface-immobilized ssDNA into the solvent, thus enhancing the hybridization process. Introduction of oligoethylene glycol into the alkanethiol prevented nonspecific binding caused by the entanglement of alkane chains. Finally, the conditions were optimized by controlling the surface charge density through the introduction of a COOH group at the alkanethiol terminus, resulting in the complete blocking of nonspecific binding and the maintenance of high hybridization efficiency.
| Original language | English |
|---|---|
| Article number | 107001 |
| Journal | Japanese Journal of Applied Physics |
| Volume | 55 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 2016 Oct 1 |
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ASJC Scopus subject areas
- Engineering(all)
- Physics and Astronomy(all)
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Optimized modification of gold nanoparticles with a self-assembled monolayer for suppression of nonspecific binding in DNA assays. / Esashika, Keiko; Saiki, Toshiharu.
In: Japanese Journal of Applied Physics, Vol. 55, No. 10, 107001, 01.10.2016.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Optimized modification of gold nanoparticles with a self-assembled monolayer for suppression of nonspecific binding in DNA assays
AU - Esashika, Keiko
AU - Saiki, Toshiharu
PY - 2016/10/1
Y1 - 2016/10/1
N2 - Homogeneous DNA assays using gold nanoparticles (AuNPs) require the reduction of nonspecific binding between AuNPs to improve sensitivity in detecting the target molecule. In this study, we employed alkanethiol self-assembled monolayers (SAMs) for modifying the AuNP surface to attain both good dispersability and high hybridization efficiency. The alkanethiol SAMs enhance the repulsive interaction between AuNPs, reducing nonspecific binding and promoting the extension of surface-immobilized ssDNA into the solvent, thus enhancing the hybridization process. Introduction of oligoethylene glycol into the alkanethiol prevented nonspecific binding caused by the entanglement of alkane chains. Finally, the conditions were optimized by controlling the surface charge density through the introduction of a COOH group at the alkanethiol terminus, resulting in the complete blocking of nonspecific binding and the maintenance of high hybridization efficiency.
AB - Homogeneous DNA assays using gold nanoparticles (AuNPs) require the reduction of nonspecific binding between AuNPs to improve sensitivity in detecting the target molecule. In this study, we employed alkanethiol self-assembled monolayers (SAMs) for modifying the AuNP surface to attain both good dispersability and high hybridization efficiency. The alkanethiol SAMs enhance the repulsive interaction between AuNPs, reducing nonspecific binding and promoting the extension of surface-immobilized ssDNA into the solvent, thus enhancing the hybridization process. Introduction of oligoethylene glycol into the alkanethiol prevented nonspecific binding caused by the entanglement of alkane chains. Finally, the conditions were optimized by controlling the surface charge density through the introduction of a COOH group at the alkanethiol terminus, resulting in the complete blocking of nonspecific binding and the maintenance of high hybridization efficiency.
UR - http://www.scopus.com/inward/record.url?scp=84989291258&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84989291258&partnerID=8YFLogxK
U2 - 10.7567/JJAP.55.107001
DO - 10.7567/JJAP.55.107001
M3 - Article
VL - 55
JO - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes
JF - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes
SN - 0021-4922
IS - 10
M1 - 107001
ER -