Optimizing Nervous System-Specific Gene Targeting with Cre Driver Lines: Prevalence of Germline Recombination and Influencing Factors

Lin Luo, Mateusz C. Ambrozkiewicz, Fritz Benseler, Cui Chen, Emilie Dumontier, Susanne Falkner, Elisabetta Furlanis, Andrea M. Gomez, Naosuke Hoshina, Wei Hsiang Huang, Mary Anne Hutchison, Yu Itoh-Maruoka, Laura A. Lavery, Wei Li, Tomohiko Maruo, Junko Motohashi, Emily Ling Lin Pai, Kenneth A. Pelkey, Ariane Pereira, Thomas PhilipsJennifer L. Sinclair, Jeff A. Stogsdill, Lisa Traunmüller, Jiexin Wang, Joke Wortel, Wenjia You, Nashat Abumaria, Kevin T. Beier, Nils Brose, Harold A. Burgess, Constance L. Cepko, Jean François Cloutier, Cagla Eroglu, Sandra Goebbels, Pascal S. Kaeser, Jeremy N. Kay, Wei Lu, Liqun Luo, Kenji Mandai, Chris J. McBain, Klaus Armin Nave, Marco A.M. Prado, Vania F. Prado, Jeffrey Rothstein, John L.R. Rubenstein, Gesine Saher, Kenji Sakimura, Joshua R. Sanes, Peter Scheiffele, Yoshimi Takai, Hisashi Umemori, Matthijs Verhage, Michisuke Yuzaki, Huda Yahya Zoghbi, Hiroshi Kawabe, Ann Marie Craig

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Luo et al. report variable rates of germline recombination in commonly used mouse Cre driver lines, influenced by sex of Cre-carrying parents and target loci. Guidelines are provided to optimize cell-type-specific recombination in genetically targeted organisms expressing site-specific recombinases.

Original languageEnglish
Pages (from-to)37-65.e5
JournalNeuron
Volume106
Issue number1
DOIs
Publication statusPublished - 2020 Apr 8

Keywords

  • Cre-lox
  • conditional gene targeting
  • conditional knockin
  • conditional knockout
  • conditional reporter
  • germline recombination
  • molecular genetics
  • mosaic recombination
  • parental sex bias
  • site-specific recombinase

ASJC Scopus subject areas

  • Neuroscience(all)

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