Optogenetic activation of serotonergic neurons enhances anxiety-like behaviour in mice

Yu Ohmura, Kenji Tanaka, Tomomi Tsunematsu, Akihiro Yamanaka, Mitsuhiro Yoshioka

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Whether increased serotonin (5-HT) release in the forebrain attenuates or enhances anxiety has been controversial for over 25 yr. Although there is considerable indirect evidence, there is no direct evidence that indicates a relationship between acute 5-HT release and anxiety. In particular, there is no known method that can reversibly, selectively, and temporally control serotonergic activity. To address this issue, we generated transgenic animals to manipulate the firing rates of central 5-HT neurons by optogenetic methods. Activation of serotonergic neurons in the median raphe nucleus was correlated to enhanced anxiety-like behaviour in mice, whereas activation of serotonergic neurons in the dorsal raphe nucleus had no effect on anxiety-like behaviour. These results indicate that an acute increase in 5-HT release from the median raphe nucleus enhances anxiety.

Original languageEnglish
Pages (from-to)1777-1783
Number of pages7
JournalInternational Journal of Neuropsychopharmacology
Volume17
Issue number11
DOIs
Publication statusPublished - 2014 May 16

Fingerprint

Optogenetics
Serotonergic Neurons
Serotonin
Anxiety
Raphe Nuclei
Genetically Modified Animals
Prosencephalon
Neurons

Keywords

  • Anxiety
  • median raphe nucleus
  • optogenetics
  • serotonin (5-HT)

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology
  • Psychiatry and Mental health
  • Medicine(all)

Cite this

Optogenetic activation of serotonergic neurons enhances anxiety-like behaviour in mice. / Ohmura, Yu; Tanaka, Kenji; Tsunematsu, Tomomi; Yamanaka, Akihiro; Yoshioka, Mitsuhiro.

In: International Journal of Neuropsychopharmacology, Vol. 17, No. 11, 16.05.2014, p. 1777-1783.

Research output: Contribution to journalArticle

Ohmura, Yu ; Tanaka, Kenji ; Tsunematsu, Tomomi ; Yamanaka, Akihiro ; Yoshioka, Mitsuhiro. / Optogenetic activation of serotonergic neurons enhances anxiety-like behaviour in mice. In: International Journal of Neuropsychopharmacology. 2014 ; Vol. 17, No. 11. pp. 1777-1783.
@article{3b808d0a77cc43fc9c05ef7cda658e59,
title = "Optogenetic activation of serotonergic neurons enhances anxiety-like behaviour in mice",
abstract = "Whether increased serotonin (5-HT) release in the forebrain attenuates or enhances anxiety has been controversial for over 25 yr. Although there is considerable indirect evidence, there is no direct evidence that indicates a relationship between acute 5-HT release and anxiety. In particular, there is no known method that can reversibly, selectively, and temporally control serotonergic activity. To address this issue, we generated transgenic animals to manipulate the firing rates of central 5-HT neurons by optogenetic methods. Activation of serotonergic neurons in the median raphe nucleus was correlated to enhanced anxiety-like behaviour in mice, whereas activation of serotonergic neurons in the dorsal raphe nucleus had no effect on anxiety-like behaviour. These results indicate that an acute increase in 5-HT release from the median raphe nucleus enhances anxiety.",
keywords = "Anxiety, median raphe nucleus, optogenetics, serotonin (5-HT)",
author = "Yu Ohmura and Kenji Tanaka and Tomomi Tsunematsu and Akihiro Yamanaka and Mitsuhiro Yoshioka",
year = "2014",
month = "5",
day = "16",
doi = "10.1017/S1461145714000637",
language = "English",
volume = "17",
pages = "1777--1783",
journal = "International Journal of Neuropsychopharmacology",
issn = "1461-1457",
publisher = "Cambridge University Press",
number = "11",

}

TY - JOUR

T1 - Optogenetic activation of serotonergic neurons enhances anxiety-like behaviour in mice

AU - Ohmura, Yu

AU - Tanaka, Kenji

AU - Tsunematsu, Tomomi

AU - Yamanaka, Akihiro

AU - Yoshioka, Mitsuhiro

PY - 2014/5/16

Y1 - 2014/5/16

N2 - Whether increased serotonin (5-HT) release in the forebrain attenuates or enhances anxiety has been controversial for over 25 yr. Although there is considerable indirect evidence, there is no direct evidence that indicates a relationship between acute 5-HT release and anxiety. In particular, there is no known method that can reversibly, selectively, and temporally control serotonergic activity. To address this issue, we generated transgenic animals to manipulate the firing rates of central 5-HT neurons by optogenetic methods. Activation of serotonergic neurons in the median raphe nucleus was correlated to enhanced anxiety-like behaviour in mice, whereas activation of serotonergic neurons in the dorsal raphe nucleus had no effect on anxiety-like behaviour. These results indicate that an acute increase in 5-HT release from the median raphe nucleus enhances anxiety.

AB - Whether increased serotonin (5-HT) release in the forebrain attenuates or enhances anxiety has been controversial for over 25 yr. Although there is considerable indirect evidence, there is no direct evidence that indicates a relationship between acute 5-HT release and anxiety. In particular, there is no known method that can reversibly, selectively, and temporally control serotonergic activity. To address this issue, we generated transgenic animals to manipulate the firing rates of central 5-HT neurons by optogenetic methods. Activation of serotonergic neurons in the median raphe nucleus was correlated to enhanced anxiety-like behaviour in mice, whereas activation of serotonergic neurons in the dorsal raphe nucleus had no effect on anxiety-like behaviour. These results indicate that an acute increase in 5-HT release from the median raphe nucleus enhances anxiety.

KW - Anxiety

KW - median raphe nucleus

KW - optogenetics

KW - serotonin (5-HT)

UR - http://www.scopus.com/inward/record.url?scp=84929939897&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84929939897&partnerID=8YFLogxK

U2 - 10.1017/S1461145714000637

DO - 10.1017/S1461145714000637

M3 - Article

C2 - 24834486

AN - SCOPUS:84929939897

VL - 17

SP - 1777

EP - 1783

JO - International Journal of Neuropsychopharmacology

JF - International Journal of Neuropsychopharmacology

SN - 1461-1457

IS - 11

ER -