TY - CHAP
T1 - Optogenetics in Caenorhabditis elegans
AU - Tsukada, Yuki
AU - Mori, Ikue
N1 - Funding Information:
This work was supported by Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Scientific Research Grant Numbers 26560459 and 19H05644.
Publisher Copyright:
© Springer Nature Singapore Pte Ltd. 2021.
PY - 2021
Y1 - 2021
N2 - With a compact neural circuit consisting of entirely mapped 302 neurons, Caenorhabditis elegans plays an important role in the development and application of optogenetics. Optogenetics in C. elegans offers the opportunity that drastically changes experimental designs with increasing accessibility for neural activity and various cellular processes, thereby accelerating the studies on the functions of neural circuits and multicellular systems. Combining optogenetics with other approaches such as electrophysiology increases the resolution of elucidation. In particular, technologies like patterned illumination specifically developed in combination with optogenetics provide new tools to interrogate neural functions. In this chapter, we introduce the reasons to use optogenetics in C. elegans, and discuss the technical issues raised, especially for C. elegans by revisiting our chapter in the first edition of this book. Throughout the chapter, we review early and recent milestone works using optogenetics to investigate a variety of biological systems including neural and behavioral regulation.
AB - With a compact neural circuit consisting of entirely mapped 302 neurons, Caenorhabditis elegans plays an important role in the development and application of optogenetics. Optogenetics in C. elegans offers the opportunity that drastically changes experimental designs with increasing accessibility for neural activity and various cellular processes, thereby accelerating the studies on the functions of neural circuits and multicellular systems. Combining optogenetics with other approaches such as electrophysiology increases the resolution of elucidation. In particular, technologies like patterned illumination specifically developed in combination with optogenetics provide new tools to interrogate neural functions. In this chapter, we introduce the reasons to use optogenetics in C. elegans, and discuss the technical issues raised, especially for C. elegans by revisiting our chapter in the first edition of this book. Throughout the chapter, we review early and recent milestone works using optogenetics to investigate a variety of biological systems including neural and behavioral regulation.
KW - Behavioral analysis
KW - C. elegans
KW - Caenorhabditis elegans
KW - Neural circuit
KW - Patterned illumination
KW - Photo-electrophysiology
KW - Tracking
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U2 - 10.1007/978-981-15-8763-4_20
DO - 10.1007/978-981-15-8763-4_20
M3 - Chapter
C2 - 33398823
AN - SCOPUS:85099331903
T3 - Advances in Experimental Medicine and Biology
SP - 321
EP - 334
BT - Advances in Experimental Medicine and Biology
PB - Springer
ER -