Oral crocetin administration suppressed refractive shift and axial elongation in a murine model of lens-induced myopia

Kiwako Mori, Toshihide Kurihara, Maki Miyauchi, Ayako Ishida, Xiaoyan Jiang, Shin ichi Ikeda, Hidemasa Torii, Kazuo Tsubota

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Abstract

Increased global incidence of myopia necessitates establishment of therapeutic approaches against its progression. To explore agents which may control myopia, we screened 207 types of natural compounds and chemical reagents based on an activity of a myopia suppressive factor, early growth response protein 1 (Egr-1) in vitro. Among the candidates, crocetin showed the highest and dose-dependent activation of Egr-1. For in vivo analysis, experimental myopia was induced in 3-week-old C57BL/6 J mice with −30 diopter (D) lenses for 3 weeks. Animals were fed with normal or mixed chow containing 0.003% (n = 19) and 0.03% (n = 7) of crocetin during myopia induction. Refraction and axial length were measured at 3-week-old and the 6-week-old with an infrared photorefractor and a SD-OCT system. Compared to controls (n = 14), crocetin administration showed a significant smaller change of refractive errors (−13.62 ± 8.14 vs +0.82 ± 5.81 D for 0.003%, p < 0.01, −2.00 ± 4.52 D for 0.03%, p < 0.01) and axial elongation (0.27 ± 0.03 vs 0.22 ± 0.04 mm for 0.003%, p < 0.01, 0.23 ± 0.05 mm for 0.03%, p < 0.05). These results suggest that a dietary factor crocetin may have a preventive effect against myopia progression.

Original languageEnglish
Article number295
JournalScientific Reports
Volume9
Issue number1
DOIs
Publication statusPublished - 2019 Dec 1

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Myopia
Lenses
Oral Administration
Early Growth Response Protein 1
Refractive Errors
crocetin
Incidence

ASJC Scopus subject areas

  • General

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Oral crocetin administration suppressed refractive shift and axial elongation in a murine model of lens-induced myopia. / Mori, Kiwako; Kurihara, Toshihide; Miyauchi, Maki; Ishida, Ayako; Jiang, Xiaoyan; Ikeda, Shin ichi; Torii, Hidemasa; Tsubota, Kazuo.

In: Scientific Reports, Vol. 9, No. 1, 295, 01.12.2019.

Research output: Contribution to journalArticle

Mori, Kiwako ; Kurihara, Toshihide ; Miyauchi, Maki ; Ishida, Ayako ; Jiang, Xiaoyan ; Ikeda, Shin ichi ; Torii, Hidemasa ; Tsubota, Kazuo. / Oral crocetin administration suppressed refractive shift and axial elongation in a murine model of lens-induced myopia. In: Scientific Reports. 2019 ; Vol. 9, No. 1.
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