TY - JOUR
T1 - Oral crocetin administration suppressed refractive shift and axial elongation in a murine model of lens-induced myopia
AU - Mori, Kiwako
AU - Kurihara, Toshihide
AU - Miyauchi, Maki
AU - Ishida, Ayako
AU - Jiang, Xiaoyan
AU - Ikeda, Shin ichi
AU - Torii, Hidemasa
AU - Tsubota, Kazuo
N1 - Funding Information:
The authors thank Y. Soejima, K. Nishimaki, H. Aoyagi, A. Yamaguchi, and M. Shidomi in ROHTO Pharmaceutical Co., Ltd. for their critical discussion and C. Shoda, M. Ibuki, Y. Miwa, Y. Tomita, H. Kunimi, Y. Katada; E, Yotsukura, Y. Arita, K. Takahashi and Y. Wang in the Laboratory of Photobiology, Keio University School of Medicine for their technical and administrative supports. The current study was conducted with financial support from Tsubota Laboratory, Inc., and ROHTO Pharmaceutical Co., Ltd. Patents have been applied for the design of the mouse eyeglass (application no. 2017-41349 by Keio University) and the therapeutic effects of crocetin (application no. 2017-096792 and 2017-210596 by Tsubota Laboratory, Inc. and Rohto Pharmaceutical Co., Ltd.).
Publisher Copyright:
© 2019, The Author(s).
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Increased global incidence of myopia necessitates establishment of therapeutic approaches against its progression. To explore agents which may control myopia, we screened 207 types of natural compounds and chemical reagents based on an activity of a myopia suppressive factor, early growth response protein 1 (Egr-1) in vitro. Among the candidates, crocetin showed the highest and dose-dependent activation of Egr-1. For in vivo analysis, experimental myopia was induced in 3-week-old C57BL/6 J mice with −30 diopter (D) lenses for 3 weeks. Animals were fed with normal or mixed chow containing 0.003% (n = 19) and 0.03% (n = 7) of crocetin during myopia induction. Refraction and axial length were measured at 3-week-old and the 6-week-old with an infrared photorefractor and a SD-OCT system. Compared to controls (n = 14), crocetin administration showed a significant smaller change of refractive errors (−13.62 ± 8.14 vs +0.82 ± 5.81 D for 0.003%, p < 0.01, −2.00 ± 4.52 D for 0.03%, p < 0.01) and axial elongation (0.27 ± 0.03 vs 0.22 ± 0.04 mm for 0.003%, p < 0.01, 0.23 ± 0.05 mm for 0.03%, p < 0.05). These results suggest that a dietary factor crocetin may have a preventive effect against myopia progression.
AB - Increased global incidence of myopia necessitates establishment of therapeutic approaches against its progression. To explore agents which may control myopia, we screened 207 types of natural compounds and chemical reagents based on an activity of a myopia suppressive factor, early growth response protein 1 (Egr-1) in vitro. Among the candidates, crocetin showed the highest and dose-dependent activation of Egr-1. For in vivo analysis, experimental myopia was induced in 3-week-old C57BL/6 J mice with −30 diopter (D) lenses for 3 weeks. Animals were fed with normal or mixed chow containing 0.003% (n = 19) and 0.03% (n = 7) of crocetin during myopia induction. Refraction and axial length were measured at 3-week-old and the 6-week-old with an infrared photorefractor and a SD-OCT system. Compared to controls (n = 14), crocetin administration showed a significant smaller change of refractive errors (−13.62 ± 8.14 vs +0.82 ± 5.81 D for 0.003%, p < 0.01, −2.00 ± 4.52 D for 0.03%, p < 0.01) and axial elongation (0.27 ± 0.03 vs 0.22 ± 0.04 mm for 0.003%, p < 0.01, 0.23 ± 0.05 mm for 0.03%, p < 0.05). These results suggest that a dietary factor crocetin may have a preventive effect against myopia progression.
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U2 - 10.1038/s41598-018-36576-w
DO - 10.1038/s41598-018-36576-w
M3 - Article
C2 - 30670743
AN - SCOPUS:85060371686
SN - 2045-2322
VL - 9
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 295
ER -