Orexin decreases mRNA expressions of NMDA and AMPA receptor subunits in rat primary neuron cultures

Nobuko Goto, Goro Katsuura, Ichiro Tatsuno, Toshiyuki Asaki, Shigenori Kawahara, Ken Ebihara, Yasushi Saito, Kazuwa Nakao

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Orexin is one of the orexigenic neuropeptides in the hypothalamus. Orexin neurons in the lateral hypothalamus (LH) project into the cerebral cortex and hippocampus in which the receptors are distributed in high concentrations. Therefore, to elucidate the actions of orexin in the cerebral cortex, we examined its effects on the mRNA expressions of N-methyl-d-aspartate (NMDA) receptor subunits (NR1, NR2A, NR2B) and α-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptor subunits (GluR1, GluR2) following 6-day application of orexin-A or orexin-B to rat primary cortical neuron cultures. The mRNAs of NR1 and NR2A subunits were significantly decreased by orexin-A and orexin-B at concentrations over 0.1 μM and 0.01 μM, respectively. The mRNA expression of NR2B subunit was also significantly decreased by orexin-A and orexin-B only at the concentration of 1 μM. Moreover, orexin-A and orexin-B at concentrations over 0.01 μM significantly decreased the mRNA expressions of AMPA receptor subunits, GluR1 and GluR2. The present study demonstrated that orexins significantly suppressed RNA expressions of NMDA and AMPA receptor subunits in cortical neuron cultures, suggesting that orexin may regulate the higher functions of the cerebral cortex as well as be involved in energy regulation in the hypothalamus.

Original languageEnglish
Pages (from-to)1582-1587
Number of pages6
JournalPeptides
Volume29
Issue number9
DOIs
Publication statusPublished - 2008 Sep 1
Externally publishedYes

Fingerprint

Propionates
Aspartic Acid
Neurons
Rats
Messenger RNA
Cerebral Cortex
Hypothalamus
Lateral Hypothalamic Area
bucide
Orexins
Neuropeptides
Hippocampus
RNA

Keywords

  • AMPA receptor subunits
  • mRNA
  • NMDA receptor subunits
  • Orexin-A
  • Orexin-B
  • Rat cortical neuron cultures

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

Cite this

Goto, N., Katsuura, G., Tatsuno, I., Asaki, T., Kawahara, S., Ebihara, K., ... Nakao, K. (2008). Orexin decreases mRNA expressions of NMDA and AMPA receptor subunits in rat primary neuron cultures. Peptides, 29(9), 1582-1587. https://doi.org/10.1016/j.peptides.2008.05.002

Orexin decreases mRNA expressions of NMDA and AMPA receptor subunits in rat primary neuron cultures. / Goto, Nobuko; Katsuura, Goro; Tatsuno, Ichiro; Asaki, Toshiyuki; Kawahara, Shigenori; Ebihara, Ken; Saito, Yasushi; Nakao, Kazuwa.

In: Peptides, Vol. 29, No. 9, 01.09.2008, p. 1582-1587.

Research output: Contribution to journalArticle

Goto, N, Katsuura, G, Tatsuno, I, Asaki, T, Kawahara, S, Ebihara, K, Saito, Y & Nakao, K 2008, 'Orexin decreases mRNA expressions of NMDA and AMPA receptor subunits in rat primary neuron cultures', Peptides, vol. 29, no. 9, pp. 1582-1587. https://doi.org/10.1016/j.peptides.2008.05.002
Goto, Nobuko ; Katsuura, Goro ; Tatsuno, Ichiro ; Asaki, Toshiyuki ; Kawahara, Shigenori ; Ebihara, Ken ; Saito, Yasushi ; Nakao, Kazuwa. / Orexin decreases mRNA expressions of NMDA and AMPA receptor subunits in rat primary neuron cultures. In: Peptides. 2008 ; Vol. 29, No. 9. pp. 1582-1587.
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