Organic phosphates as a new class of soluble guanylate cyclase inhibitors

Tsuneharu Suzuki, Makoto Suematsu, Ryu Makino

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

This study aimed to examine effects of varied organic phosphates on activities of soluble guanylate cyclase (sGC). The enzyme was purified from bovine lung. Physiologically relevant concentrations of ATP, 2,3-bisphosphoglyceric acid and inositol hexakisphosphate inhibited its enzyme activities under steady-state conditions as well as those determined under stimulation with S-nitroso-N-acetylpenicillamine, a nitric oxide donor, carbon monoxide or YC-1. Lineweaver-Burk plot analyses revealed that these three organic phosphates act as competitive inhibitors. Other organic phosphates such as cardiolipin and sphingomyelin but not inorganic phosphates exhibited such inhibitory actions. These results suggest that organic phosphates serve as inhibitors for sGC-dependent signaling events.

Original languageEnglish
Pages (from-to)49-53
Number of pages5
JournalFEBS Letters
Volume507
Issue number1
DOIs
Publication statusPublished - 2001 Oct 19

Keywords

  • 2,3-Bisphosphoglyceric acid
  • Adenosine triphosphate
  • Carbon monoxide
  • Nitric oxide
  • Organic phosphate
  • Soluble guanylate cyclase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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