Abstract
This paper describes an origami-inspired self-folding method to form three-dimensional (3D) microstructures of co-cultured cells. After a confluent monolayer of fibroblasts (NIH/3T3 cells) with loaded hepatocytes (HepG2 cells) was cultured onto two-dimensional (2D) microplates, degradation of the alginate sacrificial layer in the system by addition of alginate lyase triggered NIH/3T3 cells to self-fold the microplates around HepG2 cells, and then 3D cell co-culture microstructures were spontaneously formed. Using this method, we can create a large number of 3D cell co-culture microstructures swiftly with ease in the same time. We find that HepG2 cells confined in the 3D cell co-culture microstructures have an ability to enhance the secreted albumin compared to 2D system in a long culture period. The result indicates that the origami-based cell self-folding technique presented here is useful in regenerative medicine and the preclinical stage of drug development.
| Original language | English |
|---|---|
| Article number | 4556 |
| Journal | Scientific Reports |
| Volume | 8 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2018 Dec 1 |
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Cite this
Origami-based self-folding of co-cultured NIH/3T3 and HepG2 cells into 3D microstructures. / He, Qian; Okajima, Takaharu; Onoe, Hiroaki; Subagyo, Agus; Sueoka, Kazuhisa; Kuribayashi-Shigetomi, Kaori.
In: Scientific Reports, Vol. 8, No. 1, 4556, 01.12.2018.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Origami-based self-folding of co-cultured NIH/3T3 and HepG2 cells into 3D microstructures
AU - He, Qian
AU - Okajima, Takaharu
AU - Onoe, Hiroaki
AU - Subagyo, Agus
AU - Sueoka, Kazuhisa
AU - Kuribayashi-Shigetomi, Kaori
PY - 2018/12/1
Y1 - 2018/12/1
N2 - This paper describes an origami-inspired self-folding method to form three-dimensional (3D) microstructures of co-cultured cells. After a confluent monolayer of fibroblasts (NIH/3T3 cells) with loaded hepatocytes (HepG2 cells) was cultured onto two-dimensional (2D) microplates, degradation of the alginate sacrificial layer in the system by addition of alginate lyase triggered NIH/3T3 cells to self-fold the microplates around HepG2 cells, and then 3D cell co-culture microstructures were spontaneously formed. Using this method, we can create a large number of 3D cell co-culture microstructures swiftly with ease in the same time. We find that HepG2 cells confined in the 3D cell co-culture microstructures have an ability to enhance the secreted albumin compared to 2D system in a long culture period. The result indicates that the origami-based cell self-folding technique presented here is useful in regenerative medicine and the preclinical stage of drug development.
AB - This paper describes an origami-inspired self-folding method to form three-dimensional (3D) microstructures of co-cultured cells. After a confluent monolayer of fibroblasts (NIH/3T3 cells) with loaded hepatocytes (HepG2 cells) was cultured onto two-dimensional (2D) microplates, degradation of the alginate sacrificial layer in the system by addition of alginate lyase triggered NIH/3T3 cells to self-fold the microplates around HepG2 cells, and then 3D cell co-culture microstructures were spontaneously formed. Using this method, we can create a large number of 3D cell co-culture microstructures swiftly with ease in the same time. We find that HepG2 cells confined in the 3D cell co-culture microstructures have an ability to enhance the secreted albumin compared to 2D system in a long culture period. The result indicates that the origami-based cell self-folding technique presented here is useful in regenerative medicine and the preclinical stage of drug development.
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UR - http://www.scopus.com/inward/citedby.url?scp=85044194445&partnerID=8YFLogxK
U2 - 10.1038/s41598-018-22598-x
DO - 10.1038/s41598-018-22598-x
M3 - Article
C2 - 29540810
AN - SCOPUS:85044194445
VL - 8
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 4556
ER -