TY - JOUR
T1 - Origami-based self-folding of co-cultured NIH/3T3 and HepG2 cells into 3D microstructures
AU - He, Qian
AU - Okajima, Takaharu
AU - Onoe, Hiroaki
AU - Subagyo, Agus
AU - Sueoka, Kazuhisa
AU - Kuribayashi-Shigetomi, Kaori
N1 - Funding Information:
We gratefully acknowledge Professor Shoji Takeuchi at The University of Tokyo for supporting to produce a glass mask, and Professor Kazuhiko Ishihara at The University of Tokyo for providing the MPC polymer. We also sincerely acknowledge Masaru Sakuma and Kazumi Nakahata for their technical support in mask design and microplate production. This work was supported by the Grant-in-Aid for Scientific Research (B) [JSPS KAKENHI Grant Number JP26286030] from Japan Society for the Promotion of Science (JSPS) and Shiseido female research foundation. A part of this work was conducted at Hokkaido University, supported by “Nanotechnology Platform” Program of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan.
PY - 2018/12/1
Y1 - 2018/12/1
N2 - This paper describes an origami-inspired self-folding method to form three-dimensional (3D) microstructures of co-cultured cells. After a confluent monolayer of fibroblasts (NIH/3T3 cells) with loaded hepatocytes (HepG2 cells) was cultured onto two-dimensional (2D) microplates, degradation of the alginate sacrificial layer in the system by addition of alginate lyase triggered NIH/3T3 cells to self-fold the microplates around HepG2 cells, and then 3D cell co-culture microstructures were spontaneously formed. Using this method, we can create a large number of 3D cell co-culture microstructures swiftly with ease in the same time. We find that HepG2 cells confined in the 3D cell co-culture microstructures have an ability to enhance the secreted albumin compared to 2D system in a long culture period. The result indicates that the origami-based cell self-folding technique presented here is useful in regenerative medicine and the preclinical stage of drug development.
AB - This paper describes an origami-inspired self-folding method to form three-dimensional (3D) microstructures of co-cultured cells. After a confluent monolayer of fibroblasts (NIH/3T3 cells) with loaded hepatocytes (HepG2 cells) was cultured onto two-dimensional (2D) microplates, degradation of the alginate sacrificial layer in the system by addition of alginate lyase triggered NIH/3T3 cells to self-fold the microplates around HepG2 cells, and then 3D cell co-culture microstructures were spontaneously formed. Using this method, we can create a large number of 3D cell co-culture microstructures swiftly with ease in the same time. We find that HepG2 cells confined in the 3D cell co-culture microstructures have an ability to enhance the secreted albumin compared to 2D system in a long culture period. The result indicates that the origami-based cell self-folding technique presented here is useful in regenerative medicine and the preclinical stage of drug development.
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U2 - 10.1038/s41598-018-22598-x
DO - 10.1038/s41598-018-22598-x
M3 - Article
C2 - 29540810
AN - SCOPUS:85044194445
VL - 8
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 4556
ER -