Origin and co-localization of nitric oxide synthase, CGRP, PACAP, and VIP in the cerebral circulation of the rat

Lars Edvinsson, Tor Elsås, Norihiro Suzuki, Toshihiko Shimizu, Tony Jer Fu Lee

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

The origin of perivascular nerve fibres storing nitric oxide synthase (NOS) and co-localisation with perivascular neuropeptides were examined in the rat middle cerebral artery (MCA) by retrograde tracing with True Blue (TB) in combination with immunocytochemistry. Application of TB to the proximal part of the middle cerebral artery labelled nerve cell bodies ipsilaterally in the trigeminal, sphenopalatine, otic, and superior cervical ganglia. A few labelled cell bodies were seen contralaterally, suggesting bilateral innervation. In the parasympathetic sphenopalatine and otic ganglia, numerous TB-labelled cell bodies contained neuronal NOS (C- and N-terminal), vasoactive intestinal peptide (VIP), and pituitary adenylate cyclase activating peptide (PACAP). In the trigeminal ganglion, almost all TB-labelled cell bodies contained calcitonin generelated peptide (CGRP) but only a few cells contained NOS. In the superior cervical ganglion, the majority of the TB-labelled nerve cells contained neuropeptide Y (NPY) but none of them contained NOS. Removal of the ipsilateral sphenopalatine ganglion caused a slight reduction in the number of perivascular VIP-, PACAP-, and NOS-containing fibres after 3 days in the MCA while there was no difference at 2 and 4 weeks after the denervation as compared to control. This indicates that the parasympathetic VIP-, PACAP-, and NOS-immunoreactive nerve fibres in the rat MCA originate from several sources.

Original languageEnglish
Pages (from-to)221-228
Number of pages8
JournalMicroscopy Research and Technique
Volume53
Issue number3
DOIs
Publication statusPublished - 2001 May 1

Fingerprint

Cerebrovascular Circulation
vasoactive intestinal peptide
calcitonin
adenylate cyclase
Vasoactive Intestinal Peptide
Nitric oxide
Calcitonin
nitric oxide
nitric oxide synthase
Adenylyl Cyclases
Nitric Oxide Synthase
Peptides
rats
peptides
Middle Cerebral Artery
Rats
arteries
Parasympathetic Ganglia
Superior Cervical Ganglion
nerve fibers

Keywords

  • Immunocytochemistry
  • Middle cerebral artery
  • Nitric oxide synthase
  • Retrograde tracing
  • True Blue

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Anatomy
  • Instrumentation

Cite this

Origin and co-localization of nitric oxide synthase, CGRP, PACAP, and VIP in the cerebral circulation of the rat. / Edvinsson, Lars; Elsås, Tor; Suzuki, Norihiro; Shimizu, Toshihiko; Lee, Tony Jer Fu.

In: Microscopy Research and Technique, Vol. 53, No. 3, 01.05.2001, p. 221-228.

Research output: Contribution to journalArticle

Edvinsson, Lars ; Elsås, Tor ; Suzuki, Norihiro ; Shimizu, Toshihiko ; Lee, Tony Jer Fu. / Origin and co-localization of nitric oxide synthase, CGRP, PACAP, and VIP in the cerebral circulation of the rat. In: Microscopy Research and Technique. 2001 ; Vol. 53, No. 3. pp. 221-228.
@article{e9161c251e0b462297aaad571e3ec266,
title = "Origin and co-localization of nitric oxide synthase, CGRP, PACAP, and VIP in the cerebral circulation of the rat",
abstract = "The origin of perivascular nerve fibres storing nitric oxide synthase (NOS) and co-localisation with perivascular neuropeptides were examined in the rat middle cerebral artery (MCA) by retrograde tracing with True Blue (TB) in combination with immunocytochemistry. Application of TB to the proximal part of the middle cerebral artery labelled nerve cell bodies ipsilaterally in the trigeminal, sphenopalatine, otic, and superior cervical ganglia. A few labelled cell bodies were seen contralaterally, suggesting bilateral innervation. In the parasympathetic sphenopalatine and otic ganglia, numerous TB-labelled cell bodies contained neuronal NOS (C- and N-terminal), vasoactive intestinal peptide (VIP), and pituitary adenylate cyclase activating peptide (PACAP). In the trigeminal ganglion, almost all TB-labelled cell bodies contained calcitonin generelated peptide (CGRP) but only a few cells contained NOS. In the superior cervical ganglion, the majority of the TB-labelled nerve cells contained neuropeptide Y (NPY) but none of them contained NOS. Removal of the ipsilateral sphenopalatine ganglion caused a slight reduction in the number of perivascular VIP-, PACAP-, and NOS-containing fibres after 3 days in the MCA while there was no difference at 2 and 4 weeks after the denervation as compared to control. This indicates that the parasympathetic VIP-, PACAP-, and NOS-immunoreactive nerve fibres in the rat MCA originate from several sources.",
keywords = "Immunocytochemistry, Middle cerebral artery, Nitric oxide synthase, Retrograde tracing, True Blue",
author = "Lars Edvinsson and Tor Els{\aa}s and Norihiro Suzuki and Toshihiko Shimizu and Lee, {Tony Jer Fu}",
year = "2001",
month = "5",
day = "1",
doi = "10.1002/jemt.1086",
language = "English",
volume = "53",
pages = "221--228",
journal = "Microscopy Research and Technique",
issn = "1059-910X",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - Origin and co-localization of nitric oxide synthase, CGRP, PACAP, and VIP in the cerebral circulation of the rat

AU - Edvinsson, Lars

AU - Elsås, Tor

AU - Suzuki, Norihiro

AU - Shimizu, Toshihiko

AU - Lee, Tony Jer Fu

PY - 2001/5/1

Y1 - 2001/5/1

N2 - The origin of perivascular nerve fibres storing nitric oxide synthase (NOS) and co-localisation with perivascular neuropeptides were examined in the rat middle cerebral artery (MCA) by retrograde tracing with True Blue (TB) in combination with immunocytochemistry. Application of TB to the proximal part of the middle cerebral artery labelled nerve cell bodies ipsilaterally in the trigeminal, sphenopalatine, otic, and superior cervical ganglia. A few labelled cell bodies were seen contralaterally, suggesting bilateral innervation. In the parasympathetic sphenopalatine and otic ganglia, numerous TB-labelled cell bodies contained neuronal NOS (C- and N-terminal), vasoactive intestinal peptide (VIP), and pituitary adenylate cyclase activating peptide (PACAP). In the trigeminal ganglion, almost all TB-labelled cell bodies contained calcitonin generelated peptide (CGRP) but only a few cells contained NOS. In the superior cervical ganglion, the majority of the TB-labelled nerve cells contained neuropeptide Y (NPY) but none of them contained NOS. Removal of the ipsilateral sphenopalatine ganglion caused a slight reduction in the number of perivascular VIP-, PACAP-, and NOS-containing fibres after 3 days in the MCA while there was no difference at 2 and 4 weeks after the denervation as compared to control. This indicates that the parasympathetic VIP-, PACAP-, and NOS-immunoreactive nerve fibres in the rat MCA originate from several sources.

AB - The origin of perivascular nerve fibres storing nitric oxide synthase (NOS) and co-localisation with perivascular neuropeptides were examined in the rat middle cerebral artery (MCA) by retrograde tracing with True Blue (TB) in combination with immunocytochemistry. Application of TB to the proximal part of the middle cerebral artery labelled nerve cell bodies ipsilaterally in the trigeminal, sphenopalatine, otic, and superior cervical ganglia. A few labelled cell bodies were seen contralaterally, suggesting bilateral innervation. In the parasympathetic sphenopalatine and otic ganglia, numerous TB-labelled cell bodies contained neuronal NOS (C- and N-terminal), vasoactive intestinal peptide (VIP), and pituitary adenylate cyclase activating peptide (PACAP). In the trigeminal ganglion, almost all TB-labelled cell bodies contained calcitonin generelated peptide (CGRP) but only a few cells contained NOS. In the superior cervical ganglion, the majority of the TB-labelled nerve cells contained neuropeptide Y (NPY) but none of them contained NOS. Removal of the ipsilateral sphenopalatine ganglion caused a slight reduction in the number of perivascular VIP-, PACAP-, and NOS-containing fibres after 3 days in the MCA while there was no difference at 2 and 4 weeks after the denervation as compared to control. This indicates that the parasympathetic VIP-, PACAP-, and NOS-immunoreactive nerve fibres in the rat MCA originate from several sources.

KW - Immunocytochemistry

KW - Middle cerebral artery

KW - Nitric oxide synthase

KW - Retrograde tracing

KW - True Blue

UR - http://www.scopus.com/inward/record.url?scp=0035341124&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035341124&partnerID=8YFLogxK

U2 - 10.1002/jemt.1086

DO - 10.1002/jemt.1086

M3 - Article

C2 - 11301497

AN - SCOPUS:0035341124

VL - 53

SP - 221

EP - 228

JO - Microscopy Research and Technique

JF - Microscopy Research and Technique

SN - 1059-910X

IS - 3

ER -