Osmotic stress up-regulates aquaporin-3 gene expression in cultured human keratinocytes

Yoshinori Sugiyama, Yukiko Ota, Mariko Chikuma, Shintaro Inoue

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Of ten members of the aquaporin family (AQP), the mRNA expression and regulation of AQP1, AQP3, AQP4 and AQP9 in cultured human keratinocytes were examined by an RNase protection assay. AQP3 mRNA was expressed in growing and differentiating cells, while AQP9 mRNA was only detected in differentiating cells. The epidermis in skin-equivalent cultures expressed both AQP3 and AQP9 mRNA. However, neither AQP1 nor AQP4 mRNA was detectable in either monolayer or skin-equivalent cultures. Incubation of keratinocytes in sorbitol-added hypertonic medium increased AQP3 mRNA expression. This was confirmed using other solutes such as NaCl, mannitol, glucose and sucrose. The effect of sorbitol was reversible, dose-dependent and maximal at 24 h after addition. However, AQP1, AQP4 and AQP9 mRNA expression were unchanged under any of the hypertonic conditions examined. These findings indicated that osmotic stress up-regulates AQP3 gene expression in cultured keratinocytes.

Original languageEnglish
Pages (from-to)82-88
Number of pages7
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1522
Issue number2
DOIs
Publication statusPublished - 2001 Dec 3
Externally publishedYes

Fingerprint

Aquaporin 3
Osmotic Pressure
Keratinocytes
Gene expression
Up-Regulation
Gene Expression
Messenger RNA
Sorbitol
Skin
Aquaporins
Mannitol
Ribonucleases
Epidermis
Sucrose
Monolayers
Assays
Glucose

Keywords

  • Aquaporin
  • Hypertonic stress
  • Keratinocyte
  • Osmotic stress
  • RNase protection assay
  • Skin

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

Cite this

Osmotic stress up-regulates aquaporin-3 gene expression in cultured human keratinocytes. / Sugiyama, Yoshinori; Ota, Yukiko; Chikuma, Mariko; Inoue, Shintaro.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1522, No. 2, 03.12.2001, p. 82-88.

Research output: Contribution to journalArticle

@article{18f08288fbae456c8bee9f351850c9f7,
title = "Osmotic stress up-regulates aquaporin-3 gene expression in cultured human keratinocytes",
abstract = "Of ten members of the aquaporin family (AQP), the mRNA expression and regulation of AQP1, AQP3, AQP4 and AQP9 in cultured human keratinocytes were examined by an RNase protection assay. AQP3 mRNA was expressed in growing and differentiating cells, while AQP9 mRNA was only detected in differentiating cells. The epidermis in skin-equivalent cultures expressed both AQP3 and AQP9 mRNA. However, neither AQP1 nor AQP4 mRNA was detectable in either monolayer or skin-equivalent cultures. Incubation of keratinocytes in sorbitol-added hypertonic medium increased AQP3 mRNA expression. This was confirmed using other solutes such as NaCl, mannitol, glucose and sucrose. The effect of sorbitol was reversible, dose-dependent and maximal at 24 h after addition. However, AQP1, AQP4 and AQP9 mRNA expression were unchanged under any of the hypertonic conditions examined. These findings indicated that osmotic stress up-regulates AQP3 gene expression in cultured keratinocytes.",
keywords = "Aquaporin, Hypertonic stress, Keratinocyte, Osmotic stress, RNase protection assay, Skin",
author = "Yoshinori Sugiyama and Yukiko Ota and Mariko Chikuma and Shintaro Inoue",
year = "2001",
month = "12",
day = "3",
doi = "10.1016/S0167-4781(01)00320-7",
language = "English",
volume = "1522",
pages = "82--88",
journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",
issn = "0167-4781",
publisher = "Elsevier BV",
number = "2",

}

TY - JOUR

T1 - Osmotic stress up-regulates aquaporin-3 gene expression in cultured human keratinocytes

AU - Sugiyama, Yoshinori

AU - Ota, Yukiko

AU - Chikuma, Mariko

AU - Inoue, Shintaro

PY - 2001/12/3

Y1 - 2001/12/3

N2 - Of ten members of the aquaporin family (AQP), the mRNA expression and regulation of AQP1, AQP3, AQP4 and AQP9 in cultured human keratinocytes were examined by an RNase protection assay. AQP3 mRNA was expressed in growing and differentiating cells, while AQP9 mRNA was only detected in differentiating cells. The epidermis in skin-equivalent cultures expressed both AQP3 and AQP9 mRNA. However, neither AQP1 nor AQP4 mRNA was detectable in either monolayer or skin-equivalent cultures. Incubation of keratinocytes in sorbitol-added hypertonic medium increased AQP3 mRNA expression. This was confirmed using other solutes such as NaCl, mannitol, glucose and sucrose. The effect of sorbitol was reversible, dose-dependent and maximal at 24 h after addition. However, AQP1, AQP4 and AQP9 mRNA expression were unchanged under any of the hypertonic conditions examined. These findings indicated that osmotic stress up-regulates AQP3 gene expression in cultured keratinocytes.

AB - Of ten members of the aquaporin family (AQP), the mRNA expression and regulation of AQP1, AQP3, AQP4 and AQP9 in cultured human keratinocytes were examined by an RNase protection assay. AQP3 mRNA was expressed in growing and differentiating cells, while AQP9 mRNA was only detected in differentiating cells. The epidermis in skin-equivalent cultures expressed both AQP3 and AQP9 mRNA. However, neither AQP1 nor AQP4 mRNA was detectable in either monolayer or skin-equivalent cultures. Incubation of keratinocytes in sorbitol-added hypertonic medium increased AQP3 mRNA expression. This was confirmed using other solutes such as NaCl, mannitol, glucose and sucrose. The effect of sorbitol was reversible, dose-dependent and maximal at 24 h after addition. However, AQP1, AQP4 and AQP9 mRNA expression were unchanged under any of the hypertonic conditions examined. These findings indicated that osmotic stress up-regulates AQP3 gene expression in cultured keratinocytes.

KW - Aquaporin

KW - Hypertonic stress

KW - Keratinocyte

KW - Osmotic stress

KW - RNase protection assay

KW - Skin

UR - http://www.scopus.com/inward/record.url?scp=0035803910&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035803910&partnerID=8YFLogxK

U2 - 10.1016/S0167-4781(01)00320-7

DO - 10.1016/S0167-4781(01)00320-7

M3 - Article

C2 - 11750058

AN - SCOPUS:0035803910

VL - 1522

SP - 82

EP - 88

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

SN - 0167-4781

IS - 2

ER -