Osteoclast lineage commitment of bone marrow precursors through expression of membrane-bound TRANCE

J. M. Lean, Koichi Matsuo, S. W. Fox, K. Fuller, F. M. Gibson, G. Draycott, M. R. Wani, K. E. Bayley, B. R. Wong, Y. Choi, E. F. Wagner, T. J. Chambers

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Osteoclast formation from hemopoietic precursors is induced by TRANCE (also called RANKL, ODF, and OPGL), a membrane-bound ligand expressed by bone marrow stromal cells. Because soluble recombinant TRANCE is a suboptimal osteoclastogenic stimulus, and to eliminate the need for such dependence on stromal cells, membrane-bound TRANCE was expressed in hematopoietic precursors using retroviral gene transfer. Four TRANCE-expressing osteoclast cell lines were established that continuously generate large numbers of multinucleated cells and express tartrate-resistant acid phosphatase and calcitonin receptors. The multinuclear cells are long-lived and either fuse continuously with each other and with mononuclear cells to form enormous syncytia, or separate to form daughter multinuclear cells. When formed on bone, but not on plastic, the majority of multinuclear cells develop actin rings on bone, and resorb bone, suggesting that bone matrix may provide additional signals that facilitate osteoclastic functional maturation. Surprisingly, multinuclear cells originate from fusion of proliferating mononuclear cells that strongly express the mature macrophage markers F4/80 and Fc receptor, which are not expressed by osteoclasts. These results indicate that osteoclasts can be derived from F4/80-positive and Fc receptor-positive cells, and that TRANCE induces osteoclastic differentiation partly by suppressing the macrophage phenotype. Copyright (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)29-40
Number of pages12
JournalBone
Volume27
Issue number1
DOIs
Publication statusPublished - 2000 Jul
Externally publishedYes

Fingerprint

Osteoclasts
Bone Marrow
Membranes
Fc Receptors
Bone and Bones
Calcitonin Receptors
Macrophages
Bone Matrix
Cell Fusion
Giant Cells
Stromal Cells
Mesenchymal Stromal Cells
Plastics
Actins
Cell Count
Cell Membrane
Ligands
Phenotype
Cell Line
Genes

Keywords

  • Cell line
  • Cell lineage
  • Macrophage
  • Osteoclast
  • TRANCE

ASJC Scopus subject areas

  • Physiology
  • Hematology

Cite this

Lean, J. M., Matsuo, K., Fox, S. W., Fuller, K., Gibson, F. M., Draycott, G., ... Chambers, T. J. (2000). Osteoclast lineage commitment of bone marrow precursors through expression of membrane-bound TRANCE. Bone, 27(1), 29-40. https://doi.org/10.1016/S8756-3282(00)00306-9

Osteoclast lineage commitment of bone marrow precursors through expression of membrane-bound TRANCE. / Lean, J. M.; Matsuo, Koichi; Fox, S. W.; Fuller, K.; Gibson, F. M.; Draycott, G.; Wani, M. R.; Bayley, K. E.; Wong, B. R.; Choi, Y.; Wagner, E. F.; Chambers, T. J.

In: Bone, Vol. 27, No. 1, 07.2000, p. 29-40.

Research output: Contribution to journalArticle

Lean, JM, Matsuo, K, Fox, SW, Fuller, K, Gibson, FM, Draycott, G, Wani, MR, Bayley, KE, Wong, BR, Choi, Y, Wagner, EF & Chambers, TJ 2000, 'Osteoclast lineage commitment of bone marrow precursors through expression of membrane-bound TRANCE', Bone, vol. 27, no. 1, pp. 29-40. https://doi.org/10.1016/S8756-3282(00)00306-9
Lean, J. M. ; Matsuo, Koichi ; Fox, S. W. ; Fuller, K. ; Gibson, F. M. ; Draycott, G. ; Wani, M. R. ; Bayley, K. E. ; Wong, B. R. ; Choi, Y. ; Wagner, E. F. ; Chambers, T. J. / Osteoclast lineage commitment of bone marrow precursors through expression of membrane-bound TRANCE. In: Bone. 2000 ; Vol. 27, No. 1. pp. 29-40.
@article{c673d9cce8dc4f11ae8d9892ab18db80,
title = "Osteoclast lineage commitment of bone marrow precursors through expression of membrane-bound TRANCE",
abstract = "Osteoclast formation from hemopoietic precursors is induced by TRANCE (also called RANKL, ODF, and OPGL), a membrane-bound ligand expressed by bone marrow stromal cells. Because soluble recombinant TRANCE is a suboptimal osteoclastogenic stimulus, and to eliminate the need for such dependence on stromal cells, membrane-bound TRANCE was expressed in hematopoietic precursors using retroviral gene transfer. Four TRANCE-expressing osteoclast cell lines were established that continuously generate large numbers of multinucleated cells and express tartrate-resistant acid phosphatase and calcitonin receptors. The multinuclear cells are long-lived and either fuse continuously with each other and with mononuclear cells to form enormous syncytia, or separate to form daughter multinuclear cells. When formed on bone, but not on plastic, the majority of multinuclear cells develop actin rings on bone, and resorb bone, suggesting that bone matrix may provide additional signals that facilitate osteoclastic functional maturation. Surprisingly, multinuclear cells originate from fusion of proliferating mononuclear cells that strongly express the mature macrophage markers F4/80 and Fc receptor, which are not expressed by osteoclasts. These results indicate that osteoclasts can be derived from F4/80-positive and Fc receptor-positive cells, and that TRANCE induces osteoclastic differentiation partly by suppressing the macrophage phenotype. Copyright (C) 2000 Elsevier Science Inc.",
keywords = "Cell line, Cell lineage, Macrophage, Osteoclast, TRANCE",
author = "Lean, {J. M.} and Koichi Matsuo and Fox, {S. W.} and K. Fuller and Gibson, {F. M.} and G. Draycott and Wani, {M. R.} and Bayley, {K. E.} and Wong, {B. R.} and Y. Choi and Wagner, {E. F.} and Chambers, {T. J.}",
year = "2000",
month = "7",
doi = "10.1016/S8756-3282(00)00306-9",
language = "English",
volume = "27",
pages = "29--40",
journal = "Bone",
issn = "8756-3282",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - Osteoclast lineage commitment of bone marrow precursors through expression of membrane-bound TRANCE

AU - Lean, J. M.

AU - Matsuo, Koichi

AU - Fox, S. W.

AU - Fuller, K.

AU - Gibson, F. M.

AU - Draycott, G.

AU - Wani, M. R.

AU - Bayley, K. E.

AU - Wong, B. R.

AU - Choi, Y.

AU - Wagner, E. F.

AU - Chambers, T. J.

PY - 2000/7

Y1 - 2000/7

N2 - Osteoclast formation from hemopoietic precursors is induced by TRANCE (also called RANKL, ODF, and OPGL), a membrane-bound ligand expressed by bone marrow stromal cells. Because soluble recombinant TRANCE is a suboptimal osteoclastogenic stimulus, and to eliminate the need for such dependence on stromal cells, membrane-bound TRANCE was expressed in hematopoietic precursors using retroviral gene transfer. Four TRANCE-expressing osteoclast cell lines were established that continuously generate large numbers of multinucleated cells and express tartrate-resistant acid phosphatase and calcitonin receptors. The multinuclear cells are long-lived and either fuse continuously with each other and with mononuclear cells to form enormous syncytia, or separate to form daughter multinuclear cells. When formed on bone, but not on plastic, the majority of multinuclear cells develop actin rings on bone, and resorb bone, suggesting that bone matrix may provide additional signals that facilitate osteoclastic functional maturation. Surprisingly, multinuclear cells originate from fusion of proliferating mononuclear cells that strongly express the mature macrophage markers F4/80 and Fc receptor, which are not expressed by osteoclasts. These results indicate that osteoclasts can be derived from F4/80-positive and Fc receptor-positive cells, and that TRANCE induces osteoclastic differentiation partly by suppressing the macrophage phenotype. Copyright (C) 2000 Elsevier Science Inc.

AB - Osteoclast formation from hemopoietic precursors is induced by TRANCE (also called RANKL, ODF, and OPGL), a membrane-bound ligand expressed by bone marrow stromal cells. Because soluble recombinant TRANCE is a suboptimal osteoclastogenic stimulus, and to eliminate the need for such dependence on stromal cells, membrane-bound TRANCE was expressed in hematopoietic precursors using retroviral gene transfer. Four TRANCE-expressing osteoclast cell lines were established that continuously generate large numbers of multinucleated cells and express tartrate-resistant acid phosphatase and calcitonin receptors. The multinuclear cells are long-lived and either fuse continuously with each other and with mononuclear cells to form enormous syncytia, or separate to form daughter multinuclear cells. When formed on bone, but not on plastic, the majority of multinuclear cells develop actin rings on bone, and resorb bone, suggesting that bone matrix may provide additional signals that facilitate osteoclastic functional maturation. Surprisingly, multinuclear cells originate from fusion of proliferating mononuclear cells that strongly express the mature macrophage markers F4/80 and Fc receptor, which are not expressed by osteoclasts. These results indicate that osteoclasts can be derived from F4/80-positive and Fc receptor-positive cells, and that TRANCE induces osteoclastic differentiation partly by suppressing the macrophage phenotype. Copyright (C) 2000 Elsevier Science Inc.

KW - Cell line

KW - Cell lineage

KW - Macrophage

KW - Osteoclast

KW - TRANCE

UR - http://www.scopus.com/inward/record.url?scp=18344404083&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=18344404083&partnerID=8YFLogxK

U2 - 10.1016/S8756-3282(00)00306-9

DO - 10.1016/S8756-3282(00)00306-9

M3 - Article

C2 - 10865206

AN - SCOPUS:18344404083

VL - 27

SP - 29

EP - 40

JO - Bone

JF - Bone

SN - 8756-3282

IS - 1

ER -